Farmakognozi/Makale Koleksiyonu

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https://hdl.handle.net/20.500.12395/15402

Listeleniyor 1 - 13 / 13

Fatty acid composition analysis of some apiaceae plants using gas chromatography-flame ionization detector (GC-FID)
(Selçuk Üniversitesi, 01.10.2024) Eruygur, Nuraniye; Ayaz, Fatma; Bağcı, Yavuz; Bakır, Muhammed Raşit; Kara, Hüseyin
Background/aim: The Apiaceae family, also referred to as the parsley or carrot family, comprises a diverse group of plants with significant ecological and economic importance. The fundamental building blocks of plant lipids, fatty acids are involved in a number of critical biological functions. Flame ionization detection in gas chromatography (GC-FID) is a potent analytical method widely used for the qualitative and quantitative analysis of fatty acid composition in plant samples. Materials and methods: In this study, we investigated the fatty acid profiles of several Apiaceae plants using GC-FID to elucidate their lipid composition and potential applications. Results: A wide spectrum of fatty acids was confirmed, ranging from C6:0 to C22:6. Astrodaucus orientalis leaf and fruit were found rich in palmitic acid (C16:0; 35.96% and 37.65%, respectively). Ferulago asparagifolia was determined as the richest sample in terms of poly-unsaturated fatty acids such as linoleic acid (C18:2 cis, 25.65%), alpha linoleic acid (C18:3n3, 7.42%) and eicosadienoic acid (C20:2, 32.66%). Ferulago syriaca ethanol and hexane extracts contained considerable amount of oleic acid (C 18:1 n9, 40. 37% and 49.19%, respectively). Conclusion: Our review of the literature revealed that no prior reports have been made about the fatty acid compositions of Astrodaucus orientalis, Ferulago asparagifolia and Ferulago syriaca. As a result, the information provided here may be the first to describe the fatty acid contents of these species. Exploring the lipid profiles of these plants can enhance their potential applications in the food, pharmaceutical, and cosmetic industries.
Pharmacognostic, Physicochemical, Phytochemical Screening and In-Vitro Antioxidant Activity of Chrysochamela Noeana, Endemic in Türkiye
(Selçuk Üniversitesi, 2022) Eruygur, Nuraniye; Tekin, Mehmet; Dönmez, Erol
The aim of the present study was to conduct preliminary phytochemical screening, pharmacognostical and physicochemical investigation of Chrysochamela noeana. The fresh and dried herbs were studied by morphology, microscopy, preliminary phytochemical screening and florescence analysis of powdered drug. In addition, physicochemical parameters were studied according to WHO guidelines. Physicochemical parameters and florescence analysis were also performed. The preliminary phytochemical analysis revealed the presence of steroids, flavonoids, phenolic compounds, and carbohydrates. Total phenol contents were 245 ± 3.67, 179.06 ± 4.52, and 15.98 ± 3.32 (GAE mg/g extract) and total flavonoid content were 180.85 ± 8.21, 146.41 ± 2.56, and 13.46 ± 0.23 (QUE mg/g extract) for ethyl acetate, methanol, and water extracts, respectively. The IC50 value for extracts in ABTS radical scavenging activity were calculated in order of 51.47, 122.26 and 1146.91 µg/mL, and in DPPH for 1.55, 1.13 and 27.16 mg/mL for ethyl acetate, methanol, and water extracts, respectively. The results of these studies could be useful for correct identification and detection of adulterants from this plant material.
Anti-inflammatory activity of sesquiterpene lactones from Chrysophthalmum montanum (DC.) Boiss
(ACG PUBLICATIONS, 2020) Ayaz, Fatma; Akkol, Esra Küpeli; Gören, Nezhun; Çalış, İhsan; Dereli, F. Tuğçe Gürağaç; Duman, Hayri; Choudhary, Muhammad Iqbal; Küçükboyacı, Nurgün
The aerial parts of Chrysophthalmum montanum (DC.) Boiss. (Asteraceae) is traditionally used for wound healing, as well as for the treatment of common cold, sinusitis, and other inflammatory diseases. The objectives of this study were to identify potential anti-inflammatory effects of the methanol extract, and its different polarity subextracts (n-hexane, chloroform, n-butanol, and remaining aqueous), and guaianolide-type sesquiterpene lactones [6 alpha-acetoxy-4 alpha-hydroxy-1 beta H-guaia-9.11(13)-dien-12.8 alpha-olide (1), 6 alpha-acetoxy-4 alpha-hydroxy -9 beta.10 beta-epoxy- 1 beta H-guaia-11 (13)-en-12 .8 alpha-olide (2), 4 alpha ,6 alpha-dihydroxy -1 beta,5 alpha,7 alpha H-guaia-9(10),11 (13)-dien-12,8 alpha-olide (3), and (4 alpha,5 alpha,8 beta,10 beta)-4,10-dihydroxy-1,11(13)-guaidien-12,8-olide (4)] from the aerial parts of C. montanum. In order to evaluate the anti-inflammatory activity of C. montanum, carrageenan- and PGE(2) induced hind paw edema, and acetic acid-induced increase in capillary permeability mice models were used. The methanol extract, the chloroform subextract, and compounds 3, and 4 were shown to possess anti-inflammatory activity in in vivo models at 100 mg/kg dose. The results provide a biological, and phytochemical basis for the traditional use of C. montanum aerial parts for inflammatory conditions in Turkish folk medicine.
Screening the in vitro antioxidant, antimicrobial, anticholinesterase, antidiabetic activities of endemic Achillea cucullata (Asteraceae) ethanol extract
(ELSEVIER SCIENCE BV, 2019) Eruygur, N.; Koçyiğit, U. M.; Taslimi, P.; Ataş, M.; Tekin, M.; Gülçin, I.
The Achillea genus belongs to the Asteraceae family, which is mostly found in the northern hemisphere and is comprised of 115 species in the world. In Turkish flora, there are 52 species and 58 taxa, among them half of which are recorded as endemic. To the best of our knowledge, there has been no biological activity studied in this species until now, with the exception of one study of the antimicrobial activity of certain essential oils. This study focused primarily on the determination of antioxidant, antimicrobial, and enzyme-inhibition activity of aqueous ethanol extract of Turkish endemic Achillea cucullata by in vitro methods. The extract exhibited DPPH radical scavenging activity with an IC50 value of 132.55 +/- 0.026 mu g/mL, the total phenol content was 53.807 +/- 0.059 (mg GAE/g), and the total flavonoid content was 21.372 +/- 0.026 (mg QE/g), on the dry-weight basis. Antimicrobial activity was evaluated by a micro-dilution method focused on five microorganisms; two Gram-positive [Staphylococcus aureus (ATCC 29213) and Enterococcus faecalis (ATCC 29212)], two Gram-negative [Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922)], and one fungal strain [Candida albicans (ATCC 10231)]. Results show that the MIC value for the tested microorganism was higher than 5 mg/mL. In this work, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and alpha-glucosidase enzymes were strongly inhibited by the A. cucullata extract, and the IC50 values for these enzymes were 2.4 mu g/mL, 0.26 mu g/mL, and 24.75 mu g/mL, respectively. Certain acetylcholinesterase inhibitors have been used for treatment of Alzheimer's disease in the past. alpha-Glucosidase inhibitors are strong drug candidates, as well as potential functional food agents, for deferring the postprandial absorbency of glucose. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.
In-vitro antioxidant, a-glucosidase and a-amylase inhibitory activities of crude ethanol extract and fractions of endemic Hyacinthella acutiloba K. perss. & wendelbo bulbus
(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2019) Eruygur, Nuraniye.; Dural, Emrah.; Tekin, Mehmet.; Ozpinar, Hulya.
In the present study, the antioxidant potential as well as the alpha-amylase and alpha-glucosidase inhibitory activity of different extracts of Hyacinthella acutiloba bulbus were investigated using an in vitro model. Antioxidant activity was assayed by the DPPH radical scavenging activity, total phenol content (TPC) and total flavonoid content (TFC), reducing power assay, ferric thiocyanate (FTC) and thiobarbituric acid method (TBA). Among the extracts, n-hexane and chloroform extract of H. acutiloba displayed more activity than other extracts against a amylase and alpha-glucosidase.
In vitro evaluation of the chemical composition and various biological activities of Ficus carica leaf extracts
(TURKISH PHARMACISTS ASSOC, 2019) Ergül, Mustafa.; Ergül, Merve.; Eruygur, Nuraniye.; Ataş, Mehmet.; Uçar, Esra.
Objectives: The present study aimed to investigate the inhibitory activities of enzymes related to diabetes mellitus and Alzheimer's disease of the methanol and water extracts of Ficus carica leaf extracts. The bioactive compounds and anticancer, antioxidant, and antimicrobial effects of the extracts were also investigated. Materials and Methods: The bioactive compounds in the extracts were determined by gas chromatography-mass spectrometry. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6 sulphonic acid) (ABTS) radical scavenging, total phenol and flavonoid content, ferric reducing power, and iron chelating method. The anticancer, anticholinesterase, and antimicrobial effects were investigated using the XTT assay, Ellman method, and microdilution, respectively. Results: Our results showed that between the water and methanol extracts there was a difference in terms of chemical composition. The antioxidant results suggested that both extracts have strong antioxidant activity. Similarly, both extracts showed strong alpha-glucosidase and alpha-amylase inhibition activity, while the water extract had higher inhibition activity than the methanol extract against acetylcholinesterase and butyrylcholinesterase. The methanol extract of F. carica exhibited significant anticancer activity on MDA-MB-231 cells and showed moderate antimicrobial activities against Escherichia coli and Staphylococcus aureus. Conclusion: Our results suggest that F. carica leaves could be a valuable source for developing a promising therapeutic agent in cancer, diabetes, and Alzheimer's disease.
In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium
(SPRINGER, 2019) Eruygur, Nuraniye; Uçar, Esra; Akpulat, Hüseyin Aşkın; Shahsavari, Keyhan; Safavi, Seyed Mehdi; Kahrizi, Danial
Hypericum lydium Boiss. is a perennial plant of the Hypericaceae family, which has been used in particular to treat depression. The aim of this study was to determine in vitro antioxidant, antimicrobial activities, anticholinesterase (acetylcholinesterase (AChE)/butyrylcholinesterase (BChE)), antidiabetic activities (-glucosidase/-amylase) and Tyrosinase inhibitor activity of methanol and water extracts of H. lydium. Also, gene expression has been evaluated in the shoot and root by microarray technology. So, in general, the purpose of this study is to study the active molecules such as antioxidant, antimicrobial, antidiabetic, enzymes and genes in the plant, which is the first to be reported. The experiments were conducted in a completely randomized design with three replications. In addition, gene expression was compared in the shoot and root parts. Expression profiling was carried out by microarrays. According to the results, the highest chemical components were determined in methanol extract rather than water extract. There was a difference between the obtained components. While the highest antioxidant activity was determined from the methanol extract of plant herbs for DPPH Free Radical Scavenging Activity, antioxidant activity was the same in both methanol and water extracts using the ABTS method. The methanol extract demonstrated stronger anticholinesterase (AChE and BChE) and -amylase inhibition activity. This study was complemented by the detection of antioxidant activity and some enzyme inhibition activity in the methanol extract. Microarray showed 10,784 genes had significantly different expression in root and shoot. There was a positive effect of methanol extract in respect of different activities compared to the water extract. Gene expression showed that the number of expressed genes in the root was greater than the shoot.
Estimation of in vitro antioxidant and antimicrobial activity of methanol extracts from Stevia
(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2019) Eruygur, Nuraniye.; Atas, Mehmet.; Ucar, Esra.; Ozyigit, Yasar.; Turgut, Kenan.
In this study, the efficiency of the plant age and growth period on antioxidant and antimicrobial activity of stevia (Stevia rebaudiana Bert.) was investigated. Samples were taken from S. rebaudiana plants of three different ages (2, 3 and 4 years old) and four different growth periods (H1: 1 July, H2: 1 August, H3: 1 September and H4: 1 October). Extracts of sweet herb (S. rebaudiana) were obtained by maceration method with methanol as solvent. The experiments were carried out in completely randomized design with three replications. According to results, the highest antioxidant activity (metal chelating activity of methanol extract from stevia leaf; DPPH Free Radical Scavenging Activity of methanol extract from stevia leaves) was determined from respectively No.2 sample (3 year-old plant harvested in July) and No.1 sample (2-year-old plant harvested in July). The highest antimicrobial and antifungal activity for Escherichia coli and Staphylococcus aureus was determined from No.9, No.10, and No.11 samples (in the order of 3-year-old plant harvested in September, 3-year old plant harvested in October, and 4-year-old plant harvested in November). All of the stevia samples harvested in different age and different periods were showing antimicrobial effect against Pseudomonas aeruginosa, E. faecalis and Candida albicans.
Effect of Viscum album L. ssp. austriacum (WIESP.) Vollman on metronidazole resistant and sensitive strains of Trichomonas vaginalis
(ELSEVIER, 2019) Ozpinar, H.; Ozpinar, N.; Eruygur, N.
Trichomonas vaginalis (T. vaginalis) is a parasitic protozoan that causes trichomoniasis. Metronidazole is the standard treatment for trichomoniasis; however, metronidazole-resistant strains are implicated in an increasing number of refractory cases. Therefore, the discovery of an antiprotozoal agent that is effective on metronidazole-resistant T. vaginalis will prevent major health problems. In this study, we investigated the antiprotozoal effects of leaf, fruit and body extracts of Viscum album L. ssp. austriacum (VA) on metronidazole-resistant and - sensitive T. vaginalis protozoa. The VA used in our study was collected from 20 different pine trees in September and October. The leaves, fruits and bodies of the VA plants collected were separated and dry extracts were obtained with hexane, chloroform, n-butanol, ethyl acetate and water. The minimum lethal dose (MLD) of the metronidazole-sensitive strain, T. vaginalis ATCC50148 and themetronidazole-resistant strain, T. vaginalis ATCC50143 against metronidazole was tested in comparison with the plant extracts. In addition, GC-MS analysis was performed on the plant extracts that has antiprotozoal effects. Some of the substances identified from GC-MS analysis were purchased commercially and their antiprotozoal effects were further investigated. The VA leaf and fruit ethyl acetate extracts and body butanol extract had the most effect on T. vaginalis. GC-MS qualitative analysis detected 2,4-heptadienal, 3-methylsilane, and 2-methylfuran. Of these substances, only 2-methylfuran was found to be effective on T. vaginalis strains. Our findings suggest that the 2-methylfuran found in Viscum album L. ssp. austriacum extracts may be an alternative chemotherapeutic agent to metronidazole. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.
Effective and rapid removal of cationic and anionic dyes from aqueous solutions using Elaeagnus angustifolia L. fruits as a biosorbent
(DESALINATION PUBL, 2019) Oymak, Tülay.; Eruygur, Nuraniye.
This study focused on the removal of both cationic and anionic dye from an aqueous solution using a natural product. Evaluation was made of the biosorption of methylene blue (MB) and Indigo carmine (IC) as a basic dye and an acid dye, respectively, from an aqueous solution using the dried fruits of Elaeagnus angustifolia. The experimental parameters such as pH of the solution, initial dye concentration, and contact time, were optimized to achieve quantitative removal. The quantitative biosorption of dyes was achieved as 200 mg L-1 of MB within 10 min at pH 7, 25 mg L-1 of IC within 10 min at pH 2. The maximum biosorption capacity was 344.8 mg g(-1) for MB and 9.7 mg g(-1) for IC at room temperature. The pseudo-first-order model and pseudo-second-order models were used to explain the biosorption kinetics. The second order model best explained MB and IC biosorption onto E. angustifolia. The experimental results showed that the use of E. angustifolia as a biosorbent is fast and effective, thereby indicating that it could be an attractive alternative for the removal of anionic and cationic dyes from dye wastewater.
Phytotoxicity, toxicity on brine shrimp and ınsecticidal effect of chrysophthalmum gueneri aytac & anderb. growing in Turkey
(Türkish Pharmacists Assoc, 2018) Ayaz, Fatma; Kucukboyaci, Nurgun; Bani, Baris; Sener, Bilge; Choudhary, Muhammad Iqbal
Objectives: The aim of this study was to investigate the probable toxicity on brine shrimp, phytotoxicity, and insecticidal activity of Chrysophthalmum gueneri Aytac & Anderb. Materials and Methods: The MeOH (80%) extract obtained from the whole plant of C. gueneri was fractionated through subsequent solvent extractions in increasing polarity with n-hexane, chloroform, and n-butanol. The MeOH (80%) extract and all fractions of C. gueneri were evaluated for their biologic activities using in vitro screening bioassays such as brine shrimp lethality test and phytotoxicity against Lemna minor, as well as insecticidal activity against Rhyzopertha dominica and Tribolium castaneum. Results: The findings showed that the n-hexane and chloroform fractions of the plant had significant phytotoxic activities with 100% growth inhibition (GI) at 1000 mu g/mL against L. minor. Moreover, the MeOH (80%) extract (53% GI) and n-butanol fraction (46.6% GI) of the plant had moderate phytotoxic activities at 1000 mu g/mL. Otherwise, no samples had toxicity on the brine shrimps. In addition, the remaining water fraction had low insecticidal activity with 20% mortality against T. castaneum. Conclusion: Our results show that the n-hexane and chloroform fractions of C. gueneri had potential phytotoxic effects.
Cholinesterase, ?-glucosidase, ?-amylase, and tyrosinase inhibitory effects and antioxidant activity of veronica officinalis extracts
(2018) Eruygur, Nuraniye; Uçar, Esra
In this study, inhibition ability of Veronica officinalis extracts against Alzheimer’s disease-related enzymesacetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), diabetes mellitus related enzymes ?-glucosidase and ?-amylase and antioxidant of Veronica officinalis were investigated. To the best of our knowledge, there are no previous studieson the enzyme inhibition activities of the V. officinalis extracts. For this aim, V. officinalis extracted with methanol and waterby maceration method and their antioxidant activities were evaluated by DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS [2,2?-Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging assays, total phenol, total flavonoid content, ironchelating and ferric reducing power assays. The enzyme inhibition activity was performed with 96-well plate method.According to the obtained results; the plant extracts have good antioxidant effects. In the same time, the water extract havestronger inhibition activity against AChE, BuChE and ?-glucosidase, ?-amylase, and tyrosinase than methanol extract. Theexperimental findings indicated that the water extract exerted the higher ?-glucosidase, ?-amylase and tyrosinase (82.07 2.91, 63.61 3.93 and 47.47 0.53 at 2 mg mL -1 , respectively) inhibition than reference drugs. The obtained resultsdemonstrate that this plant has a significant potential for improving pharmaceutical formulations.
Datura Stramonium L.'nin Botanik Varyetelerinin Farklı Organlarının Total Alkaloit Miktarları ve Verimi Yönünden Karşılaştırılması
(2001) Kan, Yüksel; Arslan, Neşet
Bu araştırma 1996-1997 yıllarında Konya'da yürütülmüştür. Araştırmanın amacı, boru çiçeğinin farklı dört botanik varyetesi (stramonium, inermis, tatula, godronii)'nin alkaloit özellikleri bakımından birbiri ile karşılaştırılarak en uygun botanik varyete ya da bitki organının belirlenmesidir. Araştırma sonuçlarına göre; alkaloit miktarları bakımından varyeteler arasında önemi bir fark görülmemiştir. Ancak, bitki organları alkaloit miktarları bakımından birbirine göre farklı sonuçlar vermiştir. En yüksek alkaloit miktarı 1996 yılında var. godronii, çiçeklerinde (%0.86), 1997 yılında ise var. tatula çiçeklerinde (%0.93) elde edilmiştir. Total alkaloit verimleri bakımından varyetelerin botanik organlarında önemli farklılıklar ortaya çıkmış; 1996 yılında en fazla var. inermis (2.49 kg/da) herbasından, 1997 yılında ise var. stramonium (3.39kg/da) herbasından alınmıştır.

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