Beslenme Bozukluğunun Serum Triptofan-Kinürenin Yolağı Metabolit Düzeylerine Etkisi
Yükleniyor...
Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmadaki amacımız beslenme bozukluğunun inflamasyon ile ilişkili yeni bir yolak olan
triptofan-kinürenin yolağı üzerindeki etkisini değerlendirmektir. Bu çalışma 18-65 yaşları arasında
BKİ’si 25-30 arasında (Grup 1) 54 katılımcı, BKİ’si 18’in altında (Grup 2) 31 katılımcı, BKİ’si 30’un
üstünde (Grup 3)74 katılımcı üzerinde gerçekleştirilmiştir. Triptofan yolağı metabolitleri düzeylerinin
analizi için rutin biyokimya laboratuvarımızda bulunan LC-MS/MS (tandem kütle spektrometrisi)
cihazında halihazırda kurulu olan yöntemlerin linearite çalışması yapılarak yöntem verifikasyonu
sağlanmış olup düzeyleri tespit edilmiştir.
Yaptığımız çalışmada, Grup 1’e göre hem Grup 2 hem de Grup 3’te serum kinürenin
düzeylerinin ve Kinürenin/Triptofan oranının arttığı bulunmuştur. Bu sonuçlar istatistiksel olarak
anlamlıdır (p<0,01). Triptofan düzeylerinin ise Grup 1’e kıyasla Grup 2’de azaldığı ve yine Grup 1’e
kıyasla Grup 3’te arttığı gösterilmiştir ancak bu sonuçlar istatistiksel olarak anlamlı değildir. Kinürenik
asit (KYNA) düzeyleri ise Grup 1’e kıyasla Grup 3 ve Grup 2’de anlamlı bir şekilde yüksek bulunurken
(p<0,001) Grup 3 ve Grup 2’nin kendi arasında anlamlı bir fark görülmediği saptanmıştır. Ek olarak
Grup 3’te serum kinolinik asit (QA) düzeyleri Grup 1’e göre daha yüksektir ve anlamlıdır (p<0,001).
Grup 3, Grup 2 ile karşılaştırıldığında QA düzeyleri daha yüksek bulunmuştur ve bu sonuç istatistiksel
açıdan anlamlıdır (p<0,05). 3-hidroksiantranilik asit düzeyleri her iki grupta da Grup 1’e kıyasla
yüksektir ancak bu fark istatistiksel olarak anlamlı değildir.
Çalışmamız serum örneklerinde triptofan-kinürenin yolağı metabolit düzeylerini inceleyerek
beslenme bozukluğu ile inflamatuar hastalıklar arasındaki ilişkinin aydınlatılmasına katkıda
bulunmuştur
Our aim in this study is to evaluate the effect of malnutrition on the tryptophan-kynurenine pathway, which is a new pathway associated with inflammation. This study was conducted on 54 participants between the ages of 18-65 with a BMI of 25-30 (Group 1), 31 participants with a BMI below 18 (Group 2), and 74 participants with a BMI above 30 (Group 3). . For the analysis of tryptophan pathway metabolites levels, the linearity study of the methods currently installed in the LC-MS/MS (tandem mass spectrometry) device in our routine biochemistry laboratory was performed, and the method verification was ensured and the levels were determined. In our study, it was found that serum kynurenine levels and kynurenine/tryptophan ratio increased in both Group 2 and Group 3 compared to Group 1. These results are statistically significant (p<0.01). It was shown that tryptophan levels decreased in Group 2 compared to Group 1 and increased in Group 3 compared to Group 1, but these results were not statistically significant. While kynurenic acid (KYNA) levels were found to be significantly higher in Group 3 and Group 2 compared to Group 1 (p<0.001), no significant difference was observed between Group 3 and Group 2 itself. In addition, serum quinolinic acid (QA) levels in Group 3 were higher and significant compared to Group 1 (p<0.001). QA levels were found to be higher in Group 3 compared to Group 2, and this result was statistically significant (p<0.05). 3-hydroxyanthranilic acid levels were higher in both groups compared to Group 1, but this difference was not statistically significant. Our study contributed to the clarification of the relationship between malnutrition and inflammatory diseases by examining tryptophan-kynurenine pathway metabolite levels in serum samples.
Our aim in this study is to evaluate the effect of malnutrition on the tryptophan-kynurenine pathway, which is a new pathway associated with inflammation. This study was conducted on 54 participants between the ages of 18-65 with a BMI of 25-30 (Group 1), 31 participants with a BMI below 18 (Group 2), and 74 participants with a BMI above 30 (Group 3). . For the analysis of tryptophan pathway metabolites levels, the linearity study of the methods currently installed in the LC-MS/MS (tandem mass spectrometry) device in our routine biochemistry laboratory was performed, and the method verification was ensured and the levels were determined. In our study, it was found that serum kynurenine levels and kynurenine/tryptophan ratio increased in both Group 2 and Group 3 compared to Group 1. These results are statistically significant (p<0.01). It was shown that tryptophan levels decreased in Group 2 compared to Group 1 and increased in Group 3 compared to Group 1, but these results were not statistically significant. While kynurenic acid (KYNA) levels were found to be significantly higher in Group 3 and Group 2 compared to Group 1 (p<0.001), no significant difference was observed between Group 3 and Group 2 itself. In addition, serum quinolinic acid (QA) levels in Group 3 were higher and significant compared to Group 1 (p<0.001). QA levels were found to be higher in Group 3 compared to Group 2, and this result was statistically significant (p<0.05). 3-hydroxyanthranilic acid levels were higher in both groups compared to Group 1, but this difference was not statistically significant. Our study contributed to the clarification of the relationship between malnutrition and inflammatory diseases by examining tryptophan-kynurenine pathway metabolite levels in serum samples.
Açıklama
Anahtar Kelimeler
Kinürenin yolağı, Triptofan, Yeme bozuklukları, Eating disorders, The kynurenine pathway, Tryptophan
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Erdi̇ri̇l, A. P., (2022). Beslenme Bozukluğunun Serum Triptofan-Kinürenin Yolağı Metabolit Düzeylerine Etkisi. (Yüksek Lisans Tezi). Selçuk Üniversitesi, Sağlık Bilimler Enstitüsü, Konya.
