Güney Karaman koyun ırkında genetik polimorfizmin RAPD-PCR yöntemi ile belirlenmesi
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Dosyalar
Tarih
2006-07-21
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada amaç, RAPD-PCR yöntemini kullanarak Güney Karaman koyunlarında genetik polimorfizmi belirlemektir. Bireysel genotiplerin analizi genetik benzerlik, polimorfizm ve heterozigotluk gibi populasyon içi genetik parametrelerin anlaşılması için bilgi sağlamıştır. RAPD-PCR, 8 erkek ve 8 dişi toplam 16 bireysel genotipte rasgele seçilmiş oligonükleotid primerler ile gerçekleştirildi. Bu primerlerden çoğaltılabilir ve skorlanabilir olan en uygun 10 primer seçilerek toplam 1451 DNA bandı elde edilmiştir. Bütün RAPD bantları 600-3000 bç (baz çifti) aralığında tespit edilmiştir. Toplam 147 fragmanın 133'ü polimorfik (%90.48) 14'ü monomorfik (%9.52) bulunmuştur. Populasyon içi ortalama genetik benzerlik (Bant Paylaşım Frekansı, Fxy) ve genetik uzaklık oranları sırasıyla 0.7009 ve 0.2991 olarak hesaplanmıştır. Ortalama heterozigotluk oranı 0.3273±0.1697 olarak tahmin edilmiştir.
The objective of this research was to determine the genetic polymorphism within Güney Karaman sheep by using the Ramdomly Amplified Polymorphic DNA (RAPD) method. Analysis of individual genotypes provided information for understanding within population genetic parameters such as genetic similarity, polymorphism and heterozygosity. RAPD-PCR was carried out by using randomly primed oligonucleotide, for 8 male and 8 female individual genotypes. Among the primers tested, 10 most suitable ones, in terms of repeatability and scorability, were used and 1451 DNA bands were scored. All the RAPD bands were determined to be between 600-3000 bp (base pear) in size. From 147 fragments 133 were polymorphic (%90.48) and 14 fragments were monomorphic (%9.52). Within population avarage genetic similarity (Band Sharing Frequency, Fxy) and genetic distance were calculated as 0.7001 and 0.2991, respectively. The avarage expected heterozygosity was estimated as 0.3273±0.1697.
The objective of this research was to determine the genetic polymorphism within Güney Karaman sheep by using the Ramdomly Amplified Polymorphic DNA (RAPD) method. Analysis of individual genotypes provided information for understanding within population genetic parameters such as genetic similarity, polymorphism and heterozygosity. RAPD-PCR was carried out by using randomly primed oligonucleotide, for 8 male and 8 female individual genotypes. Among the primers tested, 10 most suitable ones, in terms of repeatability and scorability, were used and 1451 DNA bands were scored. All the RAPD bands were determined to be between 600-3000 bp (base pear) in size. From 147 fragments 133 were polymorphic (%90.48) and 14 fragments were monomorphic (%9.52). Within population avarage genetic similarity (Band Sharing Frequency, Fxy) and genetic distance were calculated as 0.7001 and 0.2991, respectively. The avarage expected heterozygosity was estimated as 0.3273±0.1697.
Açıklama
Anahtar Kelimeler
Güney Karaman koyun ırkı, Polimorfizm, Güney Karaman sheep-breed, Polymorphism
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Aytekin, İ. (2006). Güney Karaman koyun ırkında genetik polimorfizmin RAPD-PCR yöntemi ile belirlenmesi. Selçuk Üniversitesi, Yayımlanmış yüksek lisans tezi, Konya.
