Oral epitel dokularda panoramik radyografinin genotoksik etkileri
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Selçuk Üniversitesi
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Amaç: X ışınları gen mutasyonları ve kromozomal aberasyonlara sebep olabilen potansiyel mutajenik ajanlardan biridir. Bu çalışmada, panoramic radyografi alınan hastaların hücre çekirdeğinde oluşabilecek hasarı gözlemlemek için mikronükleus testi uygulanmıştır. Gerec? ve Yo?ntemler: Çalışmamızda, dental tanı nedeni ile panoramik radyografi alınması gereken 30 gönüllü hastanın bukkal mukoza sürüntü hücreleri radyografi öncesi ve radyografiden 2 hafta sonra alınmıştır. Her bir sürüntüdeki 1000 hücrenin çekirdeğindeki değişiklikler ışık mikroskobu altında sayılarak kaydedilmiştir. Bulgular: Çalışmamızda, X ışını maruziyeti sonrası bukkal mukoza hücrelerinde gözlenen mikronükleus sıklığındaki artış istatistiksel olarak anlamlı bulunmuştur (p <0.001). Sitotoksisiteye ilişkin hücre çekirdeğindeki diğer değişikliklerde ise, karyoreksis ve karyoliziste istatistiksel olarak anlamlı bir artış gözlenmiş; ancak piknozisteki artış anlamlı bulunmamıştır. Sonuc?: Sonuç olarak, dental tanı ve tedavi esnasında alınan panoramic radyografi oral mukozal hücrelerde sitotoksisiteye sebep olmaktadır. Panoramik radyografi gerektiğinde uygun dozda uygulanmalıdır.
Background: X-rays are one of the potential mutagenic agents that can cause both the gene mutations and chromosomal aberrations. In this study, the micronucleus test was implemented in order to observe the damage that can occur in the cell nucleus of the patients who had taken panoramic radiographs. Methods: In our study, buccal mucosa swabs were obtained just before the radiography and two weeks after the radiography from 30 volunteer patients who had to take radiography due to dental diagnosis. Changes in the nuclei of 1000 cells of each swab sample were counted under a light microscope and recorded. Results: In our study, increase in the micronucleus frequency observed in buccal mucosa cells after X-ray exposure was found significant (p <0.001). Regarding other changes in the cell nucleus related to cytotoxicity, statistically significant increase was observed concerning karyorrhexis and karyolysis; however, the increase in pyknosis was not statistically significant. Conclusion: As a result, panoramic radiographs taken during dental diagnosis and treatment causes cytotoxicity in oral mucosal cells. Panoramic radiographs should be applied in appropriate doses if necessary.
Background: X-rays are one of the potential mutagenic agents that can cause both the gene mutations and chromosomal aberrations. In this study, the micronucleus test was implemented in order to observe the damage that can occur in the cell nucleus of the patients who had taken panoramic radiographs. Methods: In our study, buccal mucosa swabs were obtained just before the radiography and two weeks after the radiography from 30 volunteer patients who had to take radiography due to dental diagnosis. Changes in the nuclei of 1000 cells of each swab sample were counted under a light microscope and recorded. Results: In our study, increase in the micronucleus frequency observed in buccal mucosa cells after X-ray exposure was found significant (p <0.001). Regarding other changes in the cell nucleus related to cytotoxicity, statistically significant increase was observed concerning karyorrhexis and karyolysis; however, the increase in pyknosis was not statistically significant. Conclusion: As a result, panoramic radiographs taken during dental diagnosis and treatment causes cytotoxicity in oral mucosal cells. Panoramic radiographs should be applied in appropriate doses if necessary.
Description
Keywords
Hücre Hasarı, Mikronükleus Testi, Panoramik Radyografi, Cell Injury, Micronucleus Test, Panoramic Radiography
Journal or Series
Selçuk Üniversitesi Diş Hekimliği Fakültesi Dergisi
WoS Q Value
Scopus Q Value
Volume
6
Issue
4
Citation
Karabaş, H. Ç., Özcan, İ., Güler, S. D., (2019). Oral epitel dokularda panoramik radyografinin genotoksik etkileri. Selçuk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 6 (4), 155-161.
