Effect of Ankaferd blood stopper on tendon healing: An experimental study in a rat model of Achilles tendon injury
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Tarih
2015
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu in vivo çalışmada yeni geliştirilen topikal bir hemostatik ajan olan Ankaferd Kanama Durdurucu nun (ABS) sıçan Aşil tendon yaralanma modeli kullanılarak tendon iyileşmesi üzerine etkileri araştırıldı. Gereç ve yöntemler: Çalışmada 12 erkek Wistar-Albino sıçan kullanıldı. Sıçanların sağ bacakları çalışma grubu, sol bacakları kontrol grubu olarak belirlendi. Anestezi altında iki taraflı Aşil tendon yaralanmaları oluşturuldu ve cerrahi olarak tamir edildi. Cerrahi sahalara sağ taraf için 1 mL ABS, sol taraf için ise 1 mL serum fizyolojik solüsyonu uygulandı. Hayvanlara ameliyat sonrası 21. günde ötenazi yapıldı ve ekstremitelerden tendon örnekleri alındı. Histolojik analizler yarı kantitatif tendon skorlama sistemine (Bonar Sınıflaması) göre yapıldı. Bulgular: Toplam tendon iyileşmesi skorları çalışma grubunda kontrol grubuna göre anlamlı şekilde kötüydü. Tenosit morfolojisi, esas madde boyama derecesi ve vaskülarite açısından gruplar arasında anlamlı farklılık olmasa da kollajen morfolojisi skorları çalışma grubunda anlamlı şekilde kötüydü. Sonuç: Çalışmamızda, ABS uygulaması sıçanlarda tendon iyileşmesi üzerinde histolojik olarak olumsuz etkiye sahipti. Öte yandan, bu sonuçları desteklemek için ileri biyomekanik ve immünohistokimyasal çalışmalar gereklidir.
Objectives: This in vivo study aims to investigate the effects of Ankaferd Blood Stopper ® (ABS) which is a recently developed topical hemostatic agent on tendon healing using a rat Achilles tendon injury model. Materials and methods: Twelve male Wistar-Albino rats were used in the study. Right legs of the rats were assigned as the study group, and left legs were assigned as the control group. Under anesthesia, bilateral Achilles tendon injuries were created and repaired surgically. To the surgical sites, 1 mL of ABS for the right side and 1 mL of serum physiologic solution for the left side were applied. Animals were euthanized on the 21 st postoperative day, and tendon samples were collected from the extremities. Histological analyses were performed according to the semiquantitative tendon scoring system (Bonar Scale). Results: Total tendon healing scores were significantly poorer in the study group compared to the control group. Although there were no significant differences between the groups in terms of tenocyte morphology, degree of ground substance staining, and vascularity, the collagen morphology scores were significantly poorer in the study group. Conclusion: In our study, application of ABS had histologically negative effect on tendon healing in rats. However, further biomechanical and immune histochemical studies are required to support these results.
Objectives: This in vivo study aims to investigate the effects of Ankaferd Blood Stopper ® (ABS) which is a recently developed topical hemostatic agent on tendon healing using a rat Achilles tendon injury model. Materials and methods: Twelve male Wistar-Albino rats were used in the study. Right legs of the rats were assigned as the study group, and left legs were assigned as the control group. Under anesthesia, bilateral Achilles tendon injuries were created and repaired surgically. To the surgical sites, 1 mL of ABS for the right side and 1 mL of serum physiologic solution for the left side were applied. Animals were euthanized on the 21 st postoperative day, and tendon samples were collected from the extremities. Histological analyses were performed according to the semiquantitative tendon scoring system (Bonar Scale). Results: Total tendon healing scores were significantly poorer in the study group compared to the control group. Although there were no significant differences between the groups in terms of tenocyte morphology, degree of ground substance staining, and vascularity, the collagen morphology scores were significantly poorer in the study group. Conclusion: In our study, application of ABS had histologically negative effect on tendon healing in rats. However, further biomechanical and immune histochemical studies are required to support these results.
Açıklama
Anahtar Kelimeler
Ortopedi
Kaynak
Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.)
WoS Q Değeri
Scopus Q Değeri
Cilt
26
Sayı
1
