Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage

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dc.contributor.authorKara, Zeki
dc.contributor.authorSabır, Ali
dc.contributor.authorYazar, Kevser
dc.contributor.authorDoğan, Osman
dc.contributor.authorŞit, Mehmet Murat
dc.date.accessioned2023-12-22T07:19:55Z
dc.date.available2023-12-22T07:19:55Z
dc.date.issued2018en_US
dc.departmentSelçuk Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümüen_US
dc.description.abstractThe present study was conducted to polyploidy possibility induction of grapes in the experiment, apical meristem was treated by colchicine. The factorial experiment based on randomized completely design with tree replications. The factors including different colchicine concentrations (0 as control, 1 gL-1 , 2 gL1 , 3 gL-1 , 4 gL-1 , 5 gL-1 , 6 gL-1 ) and four diploid grape genotypes cv. ‘Gök Üzüm’ (Vitis vinifera L.) which came from conventional ancient time and have a perfect adaptation to the territory, and cv. ‘Trakya İlkeren’ (Vitis vinifera L.) was bred in last two decades in Turkey, and ‘Isabella’ (Vitis labrusca L.) and 41 grape rootstock [Chasselas (Vitis vinifera L.) x (Vitis berlandieri Planch.)] were used. With this purpose eight colchicine doses were dropped to meristematic part of young plantlets twice a day (at 8.30 am and 18:00 pm) during 3 days when first true leaves emerge in greenhouse (25°C during day and 20°C at night). The morphological and cytological parameters were evaluated 16 weeks after the polyploidization treatments. Based on the size and shape, and density of stomata, the number of chloroplasts in guard cells, and chromosome counting in the root tip-end indicated that these descendants were diploids 2n=2x=38 like their parents that colchicine-induced mutation was failed in tested grape genotypes for tetraploid induction. On the other hand, only in the cv. ‘Trakya İlkeren’ an aneuploidy was observed by 5 gL-1 colchicine treated plantlet. Aneuploid ‘Trakya İlkeren’ plantlet was identified as 2n = 2x = 40. It is seeming that grape genotypes hardly response to colchicine induction for genome doubling. This is the first report for colchicine-induced aneuploid in grapes.en_US
dc.identifier.citationKara, Z., Sabır, A., Yazar, K., Doğan, O., Şit, M. M., (2018). Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage. Selcuk Journal of Agriculture and Food Sciences, 32(3), 424-429. DOI: 10.15316/SJAFS.2018.117en_US
dc.identifier.doi10.15316/SJAFS.2018.117en_US
dc.identifier.endpage429en_US
dc.identifier.issn2458-8377en_US
dc.identifier.issue3en_US
dc.identifier.startpage424en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/51518
dc.identifier.volume32en_US
dc.institutionauthorKara, Zeki
dc.institutionauthorSabır, Ali
dc.institutionauthorYazar, Kevser
dc.institutionauthorDoğan, Osman
dc.institutionauthorŞit, Mehmet Murat
dc.language.isoenen_US
dc.publisherSelçuk Üniversitesien_US
dc.relation.ispartofSelcuk Journal of Agriculture and Food Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGrapevineen_US
dc.subjectSeedlingsen_US
dc.subjectChemical mutationen_US
dc.subjectPolioloidyen_US
dc.subjectAneuploidyen_US
dc.titleEffects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stageen_US
dc.typeArticleen_US

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