Ulex europaeus Lektininin Kan Grubu Bağlama Özgüllüğü
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Date
2002
Authors
Journal Title
Journal ISSN
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Publisher
Selçuk Üniversitesi Fen-Edebiyat Fakültesi
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info:eu-repo/semantics/openAccess
Abstract
Bu çalışmada, Ulex europaeus bitkisinden elde edilen Ulex europaeus Aglutinin-I (UEA-I)’in insan kan grubu bağlama özgüllüğü ölçüldü. Bu deney için U tabanlı mikrotitrasyon kabı kullanıldı. 50µg/ml lektin çözeltisi, PBS (phosphate buffered saline) ile hazırlanarak başlangıç konsantrasyonu olarak alındı ve UEA-I’in seri sulandırılması ile insan kan grubu özgüllüğü belirlendi. Sonuç olarak, UEA-I’in %2’lik A grubu eritrositlerini 25µg/ml konsantrasyonda, B grubu eritrositlerini 6.25µg/ml konsantrasyonda, AB grubu eritrositlerini 50µg/ml konsantrasyonda ve O grubu eritrositlerini 1.57µg/ml konsantrasyonda aglutine ettiği belirlendi. Bu konsantrasyonlara bakılarak, UEA-I’in O grubu insan kanı eritrositlerine karşı spesifik olduğu tespit edildi.
In this study, it was measured the human blood group binding specificity of the Ulex europaeus Agglutinin-I (UEA-I) from Ulex europaeus. U-based microtitration plate was used for this experiment. 50µg/ml lectin solution was prepared with PBS and taken as a initiation concentration, and human blood group specificity of UEA-I was determined by use the serial dilution of lectin solution. As a result, it was observed that the UEA-I agglutinated the 2% A group erythrocytes at the concentration of 25µg/ml, the B group erythrocytes at the concentration of 6.25µg/ml, the AB group erythrocytes at the concentration of 50µg/ml and O group erythrocytes at the concentration of 1.57µg/ml. According to the results, it was determined that UEA-I is specific for O human blood group erythrocyte at that concentration examined.
In this study, it was measured the human blood group binding specificity of the Ulex europaeus Agglutinin-I (UEA-I) from Ulex europaeus. U-based microtitration plate was used for this experiment. 50µg/ml lectin solution was prepared with PBS and taken as a initiation concentration, and human blood group specificity of UEA-I was determined by use the serial dilution of lectin solution. As a result, it was observed that the UEA-I agglutinated the 2% A group erythrocytes at the concentration of 25µg/ml, the B group erythrocytes at the concentration of 6.25µg/ml, the AB group erythrocytes at the concentration of 50µg/ml and O group erythrocytes at the concentration of 1.57µg/ml. According to the results, it was determined that UEA-I is specific for O human blood group erythrocyte at that concentration examined.
Description
Keywords
Lektin, İnsan kan grupları, Ulex europaeus, UEA-I, Lectin, Human blood groups, Ulex europaeus
Journal or Series
Selçuk Üniversitesi Fen-Edebiyat Fakültesi Fen Dergisi
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Volume
1
Issue
20
Citation
Ateş, A., Özgür, Y. (2002). Ulex europaeus lektininin kan grubu bağlama özgüllüğü. Selçuk Üniversitesi Fen-Edebiyat Fakültesi Fen Dergisi, 1, (20), 83-90.
