Serulein ile oluşturulan akut pankreatitte timokinonun akut faz proteinleri karaciğer enzimleri ve bazı sitokinler üzerine etkileri
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Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Çalışmada ratlarda serulein ile oluşturulan deneysel akut pankreatitte timokinon uygulamasının bazı sitokinler, akut faz proteinleri ve karaciğer enzimleri üzerine olası etkilerinin değerlendirilmesi amaçlandı. K grubundaki hayvanlara herhangi bir uygulama yapılmadı. TQ grubundaki hayvanlara 9 gün süre ile günde 20 mg/kg timokinon intraperitoneal olarak verildi. AP grubundaki hayvanlarda araştırmanın 7. gününde 50 µg/kg ve 2 saat sonra 25 µg/kg seruleinin intraperitoneal olarak uygulanmasıyla akut pankreatit oluşturuldu. AP+TQ grubundaki hayvanlara günde 20 mg/kg olmak üzere 9 gün süre ile intraperitoneal olarak timokinon uygulandı ve 7. günde timokinon uygulamasından 2 saat sonra 50 µg/kg ve 2 saat sonra 25 µg/kg seruleinin intraperitoneal olarak uygulanmasıyla akut pankreatit oluşturuldu. Alınan kan örneklerinde İnterlökin (IL)-8, Tümör Nekroz Faktör (TNF)-α, IL-6, IL-10, C-Reaktif Protein (CRP), Haptoglobin, Seruloplazmin, Aspartat Aminotransferaz (AST) ve Alanin Aminotransferaz (ALT) düzeyleri belirlendi. Çalışmada TNF-α düzeyi akut pankreatitli grupta kontrol grubuna göre anlamlı olarak yüksek bulunurken (p<0.05), timokinon uygulanan akut pankreatitli grupta TNF-α düzeyi akut pankreatitli gruba göre anlamlı olarak daha düşük bulundu (p<0.05). IL-8 ve IL-6 düzeyleri akut pankreatit grubunda kontrol grubuna göre yüksek bulundu (p<0.05). Akut pankreatitli ratlara timokinon uygulamasına bağlı olarak IL-8 ve IL-6 düzeyleri akut pankreatitli gruba göre anlamlı oranda düşük bulundu (p<0.05). Çalışmada akut pankreatitli grupta CRP, haptoglobin ve seruloplazmin düzeyleri kontrol grubuna göre anlamlı oranda yüksekti (p<0.05). Akut pankreatitli ratlara timokinon uygulamasıyla CRP ve haptoglobin düzeyleri akut pankreatitli gruba göre anlamlı oranda düşük bulunurken (p<0.05), seruloplazmin düzeyindeki farklılık önemli değildi. Akut pankreatitli grupta AST ve ALT düzeyleri kontrol grubuna göre önemli oranda artış gösterirken (p<0.05), akut pankreatitli ratlara timokinon uygulamasıyla her iki enzim düzeylerinin akut pankreatitli gruba göre anlamlı olarak düşük olduğu belirlendi (p<0.05). Çalışmada timokinon ön uygulaması yapılan akut pankreatitli ratlarda elde edilen bulgular timokinonun pankreatite bağlı olarak gelişen inflamasyonu hafiflettiği şeklinde değerlendirilebilir.
In this study, it was aimed to evaluate the possible effects of thymoquinone administration on some cytokines, acute phase proteins and liver enzymes in experimental acute pancreatitis induced with cerulein in rats. There was no application the animals in group K. Animals in TQ group were intraperitoneally given 20 mg/kg thymoquinone daily for 9 days. In the AP group animals, acute pancreatitis was induced by intraperitoneal administration of 50 µg/kg and 25 µg/kg 2 hours later of cerulein on the 7th day of the study. Animals in the AP+TQ group were intraperitoneally administered 20 mg/kg thymoquinone daily for 9 days. On the 7th day of the study, acute pancreatitis was induced by intraperitoneal administration of 50 µg/kg and 2 hours later 25 µg/kg of cerulein after 2 hours from thymoquinone administration. IL-8, TNF-α, IL-6, IL-10, CRP, Haptoglobin, Ceruloplasmin, AST and ALT levels were determined in the blood samples taken from all animals. In the study, TNF-α level was found to be significantly higher in the acute pancreatitis group compared to the control group (p<0.05), while TNF-α level was found to be significantly lower in the acute pancreatitis group treated with thymoquinone compared to the acute pancreatitis group (p<0.05). IL-8 and IL-6 levels were higher in the acute pancreatitis group compared to the control group (p<0.05). IL-8 and IL-6 levels were found to be significantly lower in rats with acute pancreatitis treated with thymoquinone compared to the group with acute pancreatitis (p<0.05). In the study, CRP, haptoglobin and ceruloplasmin levels were significantly higher in the acute pancreatitis group when compared with the control group (p<0.05). While thymoquinone administration to rats with acute pancreatitis resulted in a significant decrease in CRP and haptoglobin levels compared to the group with acute pancreatitis (p<0.05), the difference in ceruloplasmin levels was not significant. While AST and ALT levels in the acute pancreatitis group were significantly increased when compared with the control group (p<0.05), both enzyme levels in the acute pancreatitis group treated with thymoquinone administration were found to be significantly lower than the rats with acute pancreatitis (p<0.05). In the study, the findings obtained in rats with acute pancreatitis which were pre-treated with thymoquinone can be evaluated as that thymoquinone alleviates inflammation due to pancreatitis.
In this study, it was aimed to evaluate the possible effects of thymoquinone administration on some cytokines, acute phase proteins and liver enzymes in experimental acute pancreatitis induced with cerulein in rats. There was no application the animals in group K. Animals in TQ group were intraperitoneally given 20 mg/kg thymoquinone daily for 9 days. In the AP group animals, acute pancreatitis was induced by intraperitoneal administration of 50 µg/kg and 25 µg/kg 2 hours later of cerulein on the 7th day of the study. Animals in the AP+TQ group were intraperitoneally administered 20 mg/kg thymoquinone daily for 9 days. On the 7th day of the study, acute pancreatitis was induced by intraperitoneal administration of 50 µg/kg and 2 hours later 25 µg/kg of cerulein after 2 hours from thymoquinone administration. IL-8, TNF-α, IL-6, IL-10, CRP, Haptoglobin, Ceruloplasmin, AST and ALT levels were determined in the blood samples taken from all animals. In the study, TNF-α level was found to be significantly higher in the acute pancreatitis group compared to the control group (p<0.05), while TNF-α level was found to be significantly lower in the acute pancreatitis group treated with thymoquinone compared to the acute pancreatitis group (p<0.05). IL-8 and IL-6 levels were higher in the acute pancreatitis group compared to the control group (p<0.05). IL-8 and IL-6 levels were found to be significantly lower in rats with acute pancreatitis treated with thymoquinone compared to the group with acute pancreatitis (p<0.05). In the study, CRP, haptoglobin and ceruloplasmin levels were significantly higher in the acute pancreatitis group when compared with the control group (p<0.05). While thymoquinone administration to rats with acute pancreatitis resulted in a significant decrease in CRP and haptoglobin levels compared to the group with acute pancreatitis (p<0.05), the difference in ceruloplasmin levels was not significant. While AST and ALT levels in the acute pancreatitis group were significantly increased when compared with the control group (p<0.05), both enzyme levels in the acute pancreatitis group treated with thymoquinone administration were found to be significantly lower than the rats with acute pancreatitis (p<0.05). In the study, the findings obtained in rats with acute pancreatitis which were pre-treated with thymoquinone can be evaluated as that thymoquinone alleviates inflammation due to pancreatitis.
Açıklama
Anahtar Kelimeler
Serulein, Timokinon, Cerulein, Thymoquinone, Sitokin, Akut faz proteinleri, Rat, Cytokine, Acute phase protein, Rats
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Kanaç, S. (2021). Serulein ile oluşturulan akut pankreatitte timokinonun akut faz proteinleri karaciğer enzimleri ve bazı sitokinler üzerine etkileri. (Yüksek Lisans Tezi). Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, Konya.
