Kolorektal Kanserli Hastalarda Sitokrom P450 (CYP2C9 ve CYP2C19) Genetik Polimorfizm Sıklığı
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Dosyalar
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Özellikle gelişmiş ülkelerin önemli sağlık sorunlarından biri olan kolorektal kanserler, genetik ve çevresel faktörlerin katkılarıyla gelişen multifaktöriyel bir hastalıktır. Bu vaka kontrol çalışmasında kolorektal karsinoma ile CYP2C2 alt gurubunun bir üyesi olan endojen ve eksojen maddelerin karsinojenlerin ve ilaçların metabolizmasında önemli rol alan CYP2C9 ve CYP2C19 gen polimorfizmini ve aynı zamanda sigara, alkol, ailede kanser hikayesi, asbestoz maruziyeti gibi risk faktörlerini araştırdık. Çalışmaya ailesinde kanser hikayesi olmayan 85 sağlıklı gönüllü ile Meram Tıp Fakültesi Tıbbi Onkoloji ve Genel Cerrahi kliniklerine başvuran klinik ve histopatolojik olarak çoğunluğu adenokarsinom olan kolorektal kanserli 85 hasta alındı. CYP2C9 ve CYP2C19 genlerinin heterozigot ve mutant genotiplerini belirlemek için CYP2C9*2, CYP2C9*3 ve CYP2C19*2, CYP2C19*3 mutasyon saptama kitleri Light Cycler Real Time Polymerase Chain Reaction ile kullanıldı. Sonuçlar vaka ve kontrol guruplarında istatistiksel olarak karşılaştırıldı ve hesaplanan odds oranları kullanıldı. Kolorektal karsinomdaki risk faktörleri ve CYP2C9*2, CYP2C9*3 ve CYP2C19*2, CYP2C19*3 genlerinin polimorfik genotip sıklıklarının araştırıldığı bu vaka kontrol çalışmasında; kolorektal kanserli hastalar ve kontrol gurubu arasında anlamlı fark saptanamadı. (p > 0.05). Kolorektal kanserli hastalarda beyaz toprağa maruz kalanların oranı anlamlı oranda (p < 0.05) yüksek bulundu ve beyaz toprağın kolorektal kanser için bir risk faktörü olabileceği düşünüldü.
Colorectal cancers are important health problems particularly in developed countries, are multifactorial diseases developing by the effects of both genetic and environmental factors. In this case-control study, we evaluated the relationship between colorectal carcinoma and gene polymorphizms of CYP2C9 and CYP2C19 which are the members of CYP2C subfamily playing important roles in the metabolism of endogenous and exogenous compounds, and also assessed other possible relations with carcinogens and drugs as well as many other risk factors such as smoking, alcohol, family history of cancer and asbestos exposure. Eighty-five patients histopathologically diagnosed with colorectal carcinoma admitted to Departments of Medical Oncology and Surgery at Meram Medical Faculty and eighty-five healhty volunteers, who have no family history of cancer, were included in this study. Heterozygote and mutant genotypes of CYP2C9 and CYP2C19 genes were determined by CYP2C9*2, CYP2C9*3 and CYP2C19*2, CYP2C19*3 Mutation Detection Kits by Light Cycler Real Time Polymerase Chain Reaction. Results were statistically compared in these case-control subjects and odds ratios were used. There were no significant differences between patients with colorectal carcinoma and control groups. However, asbestos exposure ratio in patients with colorectal carcinoma was significantly higher (p < 0.05) than control subjects. Therefore, asbestos exposure may be considered as a risk factor for colorectal carcinoma.
Colorectal cancers are important health problems particularly in developed countries, are multifactorial diseases developing by the effects of both genetic and environmental factors. In this case-control study, we evaluated the relationship between colorectal carcinoma and gene polymorphizms of CYP2C9 and CYP2C19 which are the members of CYP2C subfamily playing important roles in the metabolism of endogenous and exogenous compounds, and also assessed other possible relations with carcinogens and drugs as well as many other risk factors such as smoking, alcohol, family history of cancer and asbestos exposure. Eighty-five patients histopathologically diagnosed with colorectal carcinoma admitted to Departments of Medical Oncology and Surgery at Meram Medical Faculty and eighty-five healhty volunteers, who have no family history of cancer, were included in this study. Heterozygote and mutant genotypes of CYP2C9 and CYP2C19 genes were determined by CYP2C9*2, CYP2C9*3 and CYP2C19*2, CYP2C19*3 Mutation Detection Kits by Light Cycler Real Time Polymerase Chain Reaction. Results were statistically compared in these case-control subjects and odds ratios were used. There were no significant differences between patients with colorectal carcinoma and control groups. However, asbestos exposure ratio in patients with colorectal carcinoma was significantly higher (p < 0.05) than control subjects. Therefore, asbestos exposure may be considered as a risk factor for colorectal carcinoma.
Açıklama
Anahtar Kelimeler
Asbestos, Colorectal carcinoma, Cyp2c9 and cyp2c19 polymorphizm, Cytochrome p450, Kolorektal kanser, Sitokrom P450, CYP2C9, CYP2C19 gen Polimorfizmi, Asbest
Kaynak
UHOD - Uluslararasi Hematoloji-Onkoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
19
Sayı
3
Künye
Büyükdoğan, M., Boruban, M. C., Artaç M., Demirel, S., (2009). Kolorektal Kanserli Hastalarda
Sitokrom P450 (CYP2C9 ve CYP2C19)
Genetik Polimorfizm Sıklığı. UHOD - Uluslararasi Hematoloji-Onkoloji Dergisi, 19(3), 134-139.
