In vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boiss. [Capacidad antioxidante in vitro y caracterización fitoquímica de Eryngium kotschyi Boiss.]

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dc.contributor.authorPaşayeva L.
dc.contributor.authorŞafak E.K.
dc.contributor.authorArıgün T.
dc.contributor.authorFatullayev H.
dc.contributor.authorTugay O.
dc.date.accessioned2020-03-26T20:20:38Z
dc.date.available2020-03-26T20:20:38Z
dc.date.issued2020
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractContext: Eryngium kotschyi is one of the species of Eryngium genus and was endemic to Turkey. It was known, that traditionally uses of this plant in the South-Western part of Turkey for the treatment of various diseases. Aims: To evaluate the antioxidant capacities of methanol extract and ethyl acetate, n-butanol and water sub-extracts as well as to determine the phytochemical composition and to quantify the major antioxidant compounds in the active sub-extract. Methods: Besides the total phenolic compounds and total flavonoid compounds, the antioxidant capacity of E. kotschyi was evaluated by DPPH., ABTS+. and FRAP methods and qualitative and quantitative determination of phytochemical constituents in active sub-extract by LC-MS/MS. Results: The highest total phenolic (173.710 ± 1.088 mg gallic acid equivalent/gextract) and total flavonoid content (86.978 ± 0.650 mg catechin equivalent/gextract) was found in ethyl acetate sub-extract of E. kotschyi (EKE) and this sub-extract showed the highest antioxidant capacity on DPPH. (IC50 = 0.264 ± 0.040 mg/mL), ABTS+. (at a concentration of 0.125 mg/mL equivalent to 0.497 µM Trolox) and FRAP (at a concentration of 1 mg/mL equivalent to 1476 ± 5.292 mmol Fe2+) tests. Preliminary examination of the mass spectrums revealed the presence of 24 phytochemical compounds in this sub-extract and 7 of them was quantified. According to quantitative analyses the main compounds of EKE sub-extract were rosmarinic acid (490.820 ± 0.703 µg/mgextract), chlorogenic acid (80.405 ± 0.170 µg/mgextract), isorhamnetin 3-O-rutinoside (72.280 ± 0.33 µg/mgextract), rutin (63.020 ± 0.052 µg/mgextract) and caffeic acid (55.153 ± 0.523 µg/mgextract). Conclusions: The data suggest that EKE possess utilizable antioxidant properties in vitro. The antioxidant capacity of this sub-extract could be due to high content of phenolic and flavonoid contents. © 2020 Journal of Pharmacy & Pharmacognosy Researchen_US
dc.description.sponsorshipFirat University Scientific Research Projects Management Unit: TLO-2019-9411 Firat University Scientific Research Projects Management Unit: TLO-2019-9411en_US
dc.description.sponsorshipARTICLE INFO Received: May 22, 2019. Received in revised form: October 6, 2019. Accepted: October 12, 2019. Available Online: October 17, 2019. Declaration of interests: The authors declare no conflict of interest. Funding: This study was supported by Erciyes University Scientific Research Projects Coordinating Unit (BAP, project number TLO-2019-9411).en_US
dc.description.sponsorshipAuthors thank to Erciyes University Scientific Research Projects Coordinating Unit (BAP, project number TLO-2019-9411) for financial support and to Erciyes University Drug Application and Research Center (ERFARMA) for LC-MS/MS facility.en_US
dc.identifier.endpage31en_US
dc.identifier.issn0719-4250en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage18en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38631
dc.identifier.volume8en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAcademic Association of Pharmaceutical Sciences from Antofagasta (ASOCIFA)en_US
dc.relation.ispartofJournal of Pharmacy and Pharmacognosy Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectABTS+.en_US
dc.subjectAntioxidant DPPH.en_US
dc.subjectEryngium kotschyien_US
dc.subjectFRAPen_US
dc.subjectLC-MS/MSen_US
dc.titleIn vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boiss. [Capacidad antioxidante in vitro y caracterización fitoquímica de Eryngium kotschyi Boiss.]en_US
dc.typeArticleen_US

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