Metamizol’ün Embriyotoksik ve Teratojenik Etkilerinin Belirlenmesi
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Dosyalar
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalıĢmada, gerektiğinde gebelikte de kullanılanılabilen güçlü analjezik, antipiretik ve
antiinflamatuar etkiye sahip metamizol (dipiron) in ovo uygulamasının embriyotoksik ve teratojenik
etkileri ile uzun kemikler (tibia, femur) üzerindeki etkilerinin belirlenmesi amaçlandı.
Bu amaçla Atak S ırkı tavuklara ait 240 adet döllü tavuk yumurtası kullanıldı. Yumurtalar
kontrol, taĢıt, 15,62 mg/kg doz, 31,25 mg/kg doz, 62,5 mg/kg doz, 125 mg/kg doz, 250 mg/kg doz ve
500 mg/kg doz metamizol olmak üzere 8 gruba ayrıldı. Kuluçkanın 21. gününde (çıkıĢ günü) her
gruptan 10 adet civciv hassas teraziyle tartılarak canlı ağırlıkları belirlendi. Civciv çıkmayan
yumurtalar açılarak grupların mortalite oranları hesaplandı. Civcivlerden sağ-sol femur ve tibia
kemikleri alındı. Kemikler, yumuĢak dokuları uzaklaĢtırıldıktan sonra anatomik referans noktaları
belirlenerek bu noktalardan 0.01 mm hassas dijital kumpasla gerekli morfometrik ölçümler yapıldı.
Elde edilen sayısal veriler istatistiksel yöntemlerle analiz edildi.
Yüksek doz (500 mg/kg) metamizol verilen grupta mortalitenin kontrol grubuna kıyasla
anlamlı olarak (p<0.05) yüksek olduğu tespit edildi. Ayrıca yüksek doz metamizol uygulamasının
iskelet geliĢimini etkileyerek, tibia ve femur uzunluklarında önemli düĢüĢler meydana getirdiği tespit
edildi. CHEST-I aĢamasında en yüksek doz (500 mg/kg) grubunda LD100 dozu belirlendi. LD50
dozunun belirlenmesi için CHEST-II aĢamasına geçildi. CHEST-II aĢamasında metamizolün tahmini
embriyotoksik LD50 değeri Probit testi yapılarak 280 (240-317) mg/kg olarak hesaplandı.
Sonuç olarak, kuluçka baĢında yumurtalara uygulanan yüksek dozlarda metamizolün
mortaliteyi arttırdığı ve iskelet sistemini etkilediği söylenebilir.
In this study, it was aimed to determine the effects of the embryotoxic and teratogenic effects of in ovo administration of metamizole (dipyrone), used during pregnancy with strong analgesic, antipyretic and anti-inflammatory effects on long bones (tibia, femur). For this purpose, 240 fertile chicken eggs of Atak S breed chickens were used. The eggs were divided into eight groups as control, vehicle, 15,62 mg/kg dose, 31,25 mg/kg dose, 62,5 mg/kg dose, 125 mg/kg dose, 250 mg/kg dose and 500 mg/kg dose metamizole. On the 21st day of incubation (the day of hatching), 10 chicks from each group were weighed with precision scales and their body weights were determined. The mortality rates of the groups were calculated by opening the eggs that did not hatch. Right-left femur and tibia bones were taken from the chicks. After bones, from soft tissues are removed, the anatomical reference points determined and necessary morphometric measurements made from these points with a 0.01 mm precision digital caliper. Data was analysed by using statistical methods. It was determined that mortality in the group given high dose (500 mg/kg) metamizole was significantly higher (p<0.05) comparing to the control group In addition, it was determined that the application of high-dose metamizole caused significant decreases in tibia and femur lengths by affecting skeletal development. In the CHEST-I stage, the LD100 dose was determined in the highest dose (500 mg/kg) group. The CHEST-II stage was started to determine the LD50 dose. Estimated embryotoxic LD50 value of metamizole in CHEST-II stage was calculated as 280 (240-317) mg/kg by using Probit test. In conclusion, it can be said that high doses of metamizole applied to eggs at the beginning of incubation increase mortality and affect the skeletal system.
In this study, it was aimed to determine the effects of the embryotoxic and teratogenic effects of in ovo administration of metamizole (dipyrone), used during pregnancy with strong analgesic, antipyretic and anti-inflammatory effects on long bones (tibia, femur). For this purpose, 240 fertile chicken eggs of Atak S breed chickens were used. The eggs were divided into eight groups as control, vehicle, 15,62 mg/kg dose, 31,25 mg/kg dose, 62,5 mg/kg dose, 125 mg/kg dose, 250 mg/kg dose and 500 mg/kg dose metamizole. On the 21st day of incubation (the day of hatching), 10 chicks from each group were weighed with precision scales and their body weights were determined. The mortality rates of the groups were calculated by opening the eggs that did not hatch. Right-left femur and tibia bones were taken from the chicks. After bones, from soft tissues are removed, the anatomical reference points determined and necessary morphometric measurements made from these points with a 0.01 mm precision digital caliper. Data was analysed by using statistical methods. It was determined that mortality in the group given high dose (500 mg/kg) metamizole was significantly higher (p<0.05) comparing to the control group In addition, it was determined that the application of high-dose metamizole caused significant decreases in tibia and femur lengths by affecting skeletal development. In the CHEST-I stage, the LD100 dose was determined in the highest dose (500 mg/kg) group. The CHEST-II stage was started to determine the LD50 dose. Estimated embryotoxic LD50 value of metamizole in CHEST-II stage was calculated as 280 (240-317) mg/kg by using Probit test. In conclusion, it can be said that high doses of metamizole applied to eggs at the beginning of incubation increase mortality and affect the skeletal system.
Açıklama
Anahtar Kelimeler
Embriyotoksisite, Metamizol, Tavuk Embriyosu, Teratojenite, Chicken Embryo, Embryotoxicity, Metamizole, Teratogenicity
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Öztürk, S., (2023). Metamizol’ün Embriyotoksik ve Teratojenik Etkilerinin Belirlenmesi. (Doktora Tezi). Selçuk Üniversitesi, Sağlık Bilimler Enstitüsü, Konya.
