Development of Method to Simultaneous Determination of Some Water and Fat Soluble Vitamins in Feeding Additives

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dc.authorid0000-0002-1263-6271en_US
dc.authorid0000-0003-1968-1857en_US
dc.authorid0000-0002-9696-5776en_US
dc.date.accessioned2020-12-21T07:56:16Z
dc.date.available2020-12-21T07:56:16Z
dc.date.issued2012en_US
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Kimya Bölümüen_US
dc.description.abstractThis study was designed in order to develop a new method to simultaneously determine the level of water and fat soluble vitamins in feed additives. In this study, we tried to measure the levels of vitamins C, B2, B6, and B12 and nicotinic acid and folic acid (i.e., water soluble vitamins) and vitamins A, D3, K3, and E (i.e., fat soluble vitamins). Several extraction procedures were performed in order to extract vitamins from tablets and the most appropriate solution for this purpose was determined as 0.01% aqueous trifluoroacetic acid, TFA (pH 3.9) and methanol, MeOH solution (20:80, v/v). Afterwards, the separation was achieved at 30ºC with retention times less than 40 min on a Phenomenex Gemini C18 110A column (150x 4.6 mm, 3.0µm particle size) in a single run with a gradient elution of mobile phase consisting of 0.01% TFA pH 4.0 (solvent A) and 100% MEOH (solvent B) at a ?ow rate of 0.7 mL min-1 and the detection was performed with diode array (DAD) at different the wavelengths which are obtained maximum signal of analyzed vitamins. Identification and quantification of compounds was achieved by comparing their retention times and peak areas with those of the vitamin standards. The lowest limit of detection (LOD) and limit of quantification (LOQ) were determined and the accuracy of the method was tested by measuring average recoveryen_US
dc.identifier.citationKucukkolbasti, S., Ires, N., Kara, H. (2012).Development of Method to Simultaneous Determination of Some Water and Fat Soluble Vitamins in Feeding Additives. Journal of Selcuk University Natural and Applied Science, 1, (4), 30-47.en_US
dc.identifier.endpage47en_US
dc.identifier.issn2147-3781en_US
dc.identifier.issue4en_US
dc.identifier.startpage30en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/40861
dc.identifier.volume1en_US
dc.institutionauthorKucukkolbasti, Semahat
dc.institutionauthorIres, Necla
dc.institutionauthorKara, Huseyin
dc.language.isoenen_US
dc.publisherSelçuk Üniversitesien_US
dc.relation.ispartofJournal of Selcuk University Natural and Applied Scienceen_US
dc.relation.publicationcategoryMakale - Ulusal - Editör Denetimli Dergien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSimultaneous determinationen_US
dc.subjectWater- and fat-soluble vitaminsen_US
dc.subjectRP-HPLC, Multivitamin tabletsen_US
dc.subjectFluorescence detectionen_US
dc.subjectDiode array detectionen_US
dc.titleDevelopment of Method to Simultaneous Determination of Some Water and Fat Soluble Vitamins in Feeding Additivesen_US
dc.typeArticleen_US

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