Otitis eksternalı köpeklerden izole edilen mikroorganizmalar ve antibiyotik duyarlılıkları
Yükleniyor...
Dosyalar
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada otitis eksternalı köpeklerden alınan kulak sıvap örneklerinde mikroorganizmaların izolasyonu ve izole edilen bakterilerin antibiyotik duyarlılıklarının belirlenmesi amaçlandı. Gereç ve Yöntem: Bu çalışmada, otitis eksterna teşhisi konulmuş 41 köpekten 58 kulak sıvap örneği toplandı. Örneklerden mikroorganizmalar konvansiyonel bakteriyolojik yöntemlerle izole ve identifiye edildi. İzole edilen bakterilerin antibiyotik duyarlılıkları agar disk difüzyon testi ile belirlendi. Bulgular: Örneklerin 52 (%89.66)’sinden 64 mikroorganizma izolasyonu yapılırken, 6 (10.34)’sından patojen etken izole edilemedi. Bu mikroorganizmalar arasında en yüksek oranda Staphylococcus aureus (%31.25) izole edildi. Ayrıca, sırasıyla koagülaz negatif stafilokok (KNS) (%14.06), Streptococcus sp. (%12.5), Corynebacterium sp. (%6.25), Proteus sp. (%4.68), Pseudomonas aeruginosa (%3.13) ve Candida sp. (%28.13) izole edildi. S. aureus, Streptococcus sp., Corynebacterium sp. ve KNS izolatlarının tümü ve Proteus sp. izolatlarının %66.67'si amoksisilin-klavulanik asite duyarlı bulundu. Bu bakterilerin enrofloksasin, amoksisilin, oksitetrasiklin, gentamisin, trimetoprimsulfametoksazol ve eritromisine duyarlılıkları sırasıyla %65.22, %52.17, %47.83, %43.48, %30.43 olarak saptandı. P. aeruginosa izolatlarının tümü enrofloksasin ve gentamisine, %50’si de oksitetrasikline duyarlı bulundu. Öneri: Bakteriyel izolatların çalışmada kullanılan antibiyotiklere duyarlılıklarındaki farklılıklar, otitis eksternalı köpeklerin tedavisi için mutlaka antibiyotik duyarlılık testlerinin yapılması gerektiğini göstermektedir. Ayrıca, çalışmada Candida sp.’nin yüksek oranda izole edilmiş olması, köpeklerin otitis eksterna olgularında mayaların da gözardı edilmemesi gerektiğini ortaya koymaktadır.
Aim: In this study, the determination of isolation of microorganisms and antibiotic susceptibility of isolated bacteria in ear swab samples collected from dogs with otitis externa were aimed. Materials and Methods: In this study, 58 ear swab samples were collected from 41 dogs with otitis externa. The microorganisms were isolated from the samples and identified by conventional bacteriological methods. The antibiotic susceptibilities of the isolates were determined by agar disc diffusion method. Results: While 64 microorganisms were isolated from 52 (88.66%) of the samples, pathogen microorganism could not be isolated from 6 (10.34%) samples. S. aureus (31.25%) was the most frequently isolated agent. Also, coagulase negative staphylococci (CNS) (14.06%), Streptococcus sp. (12.5%), Corynebacterium sp. (6.25%), Proteus sp. (4.68%), Pseudomonas aeruginosa (3.13%) and Candida sp. (28.13%) were isolated, respectively. All of the S. aureus, Streptococcus sp., Corynebacterium sp. and CNS isolates and 66.67% of Proteus sp. were susceptible to amoxicillin-clavulanic acid. This bacterial isolates were found susceptible 65.22%, 52.17%, 47.83%, 43.48%, and 30.43% to enrofloxacin, amoxicillin and oxytetracycline, gentamicin, trimethoprim-sulfamethoxazole and erythromycin, respectively. All P. aeruginosa isolates to enrofloxacin and gentamicin, and 50% of this isolates were susceptible to oxytetracycline. Conclusion: The differences in antibiotic susceptibility of the bacterial isolates shows that antibiotic susceptibility tests is necessary for treatment of dogs with otitis externa and yeasts should not be overlooked, due to high rates of Candida sp. isolation.
Aim: In this study, the determination of isolation of microorganisms and antibiotic susceptibility of isolated bacteria in ear swab samples collected from dogs with otitis externa were aimed. Materials and Methods: In this study, 58 ear swab samples were collected from 41 dogs with otitis externa. The microorganisms were isolated from the samples and identified by conventional bacteriological methods. The antibiotic susceptibilities of the isolates were determined by agar disc diffusion method. Results: While 64 microorganisms were isolated from 52 (88.66%) of the samples, pathogen microorganism could not be isolated from 6 (10.34%) samples. S. aureus (31.25%) was the most frequently isolated agent. Also, coagulase negative staphylococci (CNS) (14.06%), Streptococcus sp. (12.5%), Corynebacterium sp. (6.25%), Proteus sp. (4.68%), Pseudomonas aeruginosa (3.13%) and Candida sp. (28.13%) were isolated, respectively. All of the S. aureus, Streptococcus sp., Corynebacterium sp. and CNS isolates and 66.67% of Proteus sp. were susceptible to amoxicillin-clavulanic acid. This bacterial isolates were found susceptible 65.22%, 52.17%, 47.83%, 43.48%, and 30.43% to enrofloxacin, amoxicillin and oxytetracycline, gentamicin, trimethoprim-sulfamethoxazole and erythromycin, respectively. All P. aeruginosa isolates to enrofloxacin and gentamicin, and 50% of this isolates were susceptible to oxytetracycline. Conclusion: The differences in antibiotic susceptibility of the bacterial isolates shows that antibiotic susceptibility tests is necessary for treatment of dogs with otitis externa and yeasts should not be overlooked, due to high rates of Candida sp. isolation.
Açıklama
Anahtar Kelimeler
Otitis eksterna, köpek, antibiyotik duyarlılığı, Otitis externa, dog, antibiotic sensitivity
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
32
Sayı
2
Künye
Öztürk, D., Pehlivanoğlu, F., Türütoğlu, H., Şirin, Y. S., Şababoğlu, E., (2016). Otitis eksternalı köpeklerden izole edilen mikroorganizmalar ve antibiyotik duyarlılıkları. Eurasian Journal of Veterinary Sciences, 32 (2), 84-88. DOI:10.15312/EurasianJVetSci.2016215517
