Arı Kovanı Havası ve Polen Uygulamalarının Sigara Dumanına Maruz Bırakılan Ratlarda Canlı Ağırlık ve Bazı Biyokimyasal Parametreler Üzerine Etkilerinin Araştırılması
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Dosyalar
Tarih
2022
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Yayıncı
Selçuk Üniversitesi Sağlık Bilimler Enstitisü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışma, arı kovanı havası ve polen uygulamalarının sigara dumanına maruz bırakılan
ratlarda canlı ağırlık ve bazı biyokimyasal parametreler üzerine etkilerinin araştırılması amacıyla
gerçekleştirildi.
Çalışma 64 adet erkek Wistar Albino ırkı rat kullanılarak ve 1.Kontrol, 2.Shame (0.5 ml
distile su gavaj yolu ile), 3.Arı kovanı havası (günde 1 defa 30 dakika), 4.Polen (200 mg/kg/gün dozda
distile suda çözündürerek günde bir kez), 5.Arı kovanı havası+Polen, 6.Sigara (günde 2 defa birer
paket sigara kullanılarak toplam 2 saat sigara dumanı), 7.Sigara+Arı kovanı havası, 8.Sigara+Arı
kovanı havası+Polen olmak üzere 8 grup üzerinde gerçekleştirildi.
Kontrol grubuna göre serum düzeyleri karşılaştırıldığında trigliserit düzeylerinin 6, 7,
8.gruplarda düştüğü (p<0,05), 6.gruba göre 8.grubun total kolesterol düzeyinin yükseldiği (p<0,05),
AST aktivitesi 7. ve 8.grupta yükselirken (p<0,05), sigara grupları ele alındığında 6.gruba göre
8.grubun AST aktivitesinin daha da yükseldiği (p<0,05), 4.grubun BUN düzeyinin yükseldiği
(p<0,05), ürik asitin 7 ve 8.grupta yüksek (p<0,05) olduğu, albümin düzeyinin 7.grupta yükseldiği
(p<0,05), Pi, Zn ve Fe düzeyi 3, 5, 6, 7, 8.gruplarda yükseldiği (p<0,05), Mg düzeylerinin 7. ve
8.gruplarda yüksekdiği (p<0,05), IgG ve IgM 4.grubun düzeylerinin yükseldiği (p<0,05), belirlendi.
Kontrol grubuna göre antioksidan parametreler kıyaslandığında, MDA yönünden tüm grup
düzeylerinin benzer olduğu, 6.gruba göre 8.grupta MDA düzeyinin anlamlı olarak düştüğü (p<0,05),
CAT aktivitesi 6. ve 7.gruplarda yükseldiği (p<0,05), SOD aktivitesi 5.grupta yükseldiği (p<0,05),
6.gruba göre 8.grubun SOD aktivitesinin düştüğü (p<0,05), GSH değeri 6.grupta yükseldiği (p<0,05),
6. gruba göre 7 ve 8.grup değerlerinin düştüğü (p<0,05) görüldü.
Kontrol grubuna göre BAL sıvısı parametreleri kıyaslandığında, GGT aktivitesinin 6.grupta
yükseldiği (p<0,05), 6.gruba göre 7 ve 8.grupların enzim aktivitesinin düşüktüğü (p<0,05), ALT
aktivitesinin 7 ve 8.gruplarda yükseldiği (p<0,05), 6.gruba göre 7 ve 8. grupların ALT aktivitesinin
viii
yükseldiği (p<0,05), 3.grubun albümin ve kalsiyum düzeylerinin yüksek olduğu (p<0,05), Pi
düzeyinin 5.grupta düştüğü (p<0,05), 6.gruba göre 8.grubun Pi düzeyinin daha da düşük (p<0,05)
olduğu, Zn düzeyinin 6.grupta yükseldiği (p<0,05), 6.gruba göre 7 ve 8.grubun Zn düzeylerinin ise
düştüğü (p<0,05) belirlendi.
Sonuç olarak sigara uygulamasına ilave olarak arı kovanı havası, arı kovanı havası+polen
uygulamalarının kolesterol, ürik asit düzeyini ve AST aktivitesini arttırması, GSH düzeyini
düşürmesi, immünglobülinler üzerinde etkisinin olmaması, arı kovanı havası+polenin MDA ve SOD
aktivitelerini düşürmesi yönüyle ilgi çekici olarak bulunmuştur.
This study was carried out to investigate the effects of beehive air and pollen applications on body weight and some biochemical parameters in rats exposed to cigarette smoke. The study was carried out as 1.Control, 2.Shame (0.5 ml distilled water by gavage), 3.Beehive air (30 minutes once a day), 4.Pollen (dissolve in distilled water at a dose of 200 mg/kg/day once a day), 5.Beehive air + Pollen, 6.Smoking (a total of 2 hours of cigarette smoke by using a pack of cigarettes 2 times a day), 7.Cigarettes+ Beehive air, 8.Cigarette+Beehive air+Pollen on 8 groups of 64 male Wistar Albino rats. When the serum values of the control group were compared, the triglyceride value decreased in the 6th, 7th and 8th groups (p<0.05), and the cholesterol value of the 8th group increased compared to the 6th group (p<0.05), as the AST enzyme activity increased in the 7th and 8th groups (p<0.05), when the smoking groups were considered, the AST levels activity of the 8th group was higher than the 6th group (p<0.05) The BUN levels of the 4th group increased (p<0.05), the uric acid level was high in the 7th and 8th groups (p<0.05), and the albumin level increased in the 7th group (p<0.05) Pi, Zn and Fe levels increased in 3, 5, 6, 7, 8 groups (p<0.05), Mg value increased in 7th and 8th groups (p<0.05), and it was determined that the levels of IgG and IgM increased in 4th group (p<0.05. When antioxidant parameters were compared with the control group, MDA value was similar in all groups, MDA value decreased significantly in 8th group compared to 6th group (p<0.05), CAT enzyme activity increased in 6th and 7th groups (p<0.05), SOD enzyme activity was high in the 5th group (p<0.05), compared to the 6th group, the SOD enzyme activity of the 8th group was lower (p<0.05), GSH values were higher in the 6th group (p<0.05), and the 7th and 8th groups were lower than the 6th group (p<0.05). When the BAL fluid parameters were compared to the control group, GGT enzyme activity was high in group 6 (p<0.05), and enzyme activity in groups 7 and 8 was lower compared to group 6 (p<0.05), ALT enzyme activity was high in groups 7 and 8 (p<0.05), enzyme activity was higher in groups 7 and 8 compared to group 6 (p<0.05), albumin and Ca values were increased of the 3th group x (p<0.05), the Pi value was low in the 5th group (p<0.05), Pi value of the 8th group was even lower than the 6th group (p<0.05), Zn values were found to be high in the 6th group (p<0.05), and the Zn levels of the 7th and 8th groups were lower (p<0.05) compared to the 6th group. In addition to smoking, beehive air, beehive air+pollen applications were found to be interesting in that they increase cholesterol, AST enzyme activity, uric acid level, decrease GSH level, have no effect on immunoglobulins, and decrease MDA and SOD enzyme activities of bee hive air+pollen.
This study was carried out to investigate the effects of beehive air and pollen applications on body weight and some biochemical parameters in rats exposed to cigarette smoke. The study was carried out as 1.Control, 2.Shame (0.5 ml distilled water by gavage), 3.Beehive air (30 minutes once a day), 4.Pollen (dissolve in distilled water at a dose of 200 mg/kg/day once a day), 5.Beehive air + Pollen, 6.Smoking (a total of 2 hours of cigarette smoke by using a pack of cigarettes 2 times a day), 7.Cigarettes+ Beehive air, 8.Cigarette+Beehive air+Pollen on 8 groups of 64 male Wistar Albino rats. When the serum values of the control group were compared, the triglyceride value decreased in the 6th, 7th and 8th groups (p<0.05), and the cholesterol value of the 8th group increased compared to the 6th group (p<0.05), as the AST enzyme activity increased in the 7th and 8th groups (p<0.05), when the smoking groups were considered, the AST levels activity of the 8th group was higher than the 6th group (p<0.05) The BUN levels of the 4th group increased (p<0.05), the uric acid level was high in the 7th and 8th groups (p<0.05), and the albumin level increased in the 7th group (p<0.05) Pi, Zn and Fe levels increased in 3, 5, 6, 7, 8 groups (p<0.05), Mg value increased in 7th and 8th groups (p<0.05), and it was determined that the levels of IgG and IgM increased in 4th group (p<0.05. When antioxidant parameters were compared with the control group, MDA value was similar in all groups, MDA value decreased significantly in 8th group compared to 6th group (p<0.05), CAT enzyme activity increased in 6th and 7th groups (p<0.05), SOD enzyme activity was high in the 5th group (p<0.05), compared to the 6th group, the SOD enzyme activity of the 8th group was lower (p<0.05), GSH values were higher in the 6th group (p<0.05), and the 7th and 8th groups were lower than the 6th group (p<0.05). When the BAL fluid parameters were compared to the control group, GGT enzyme activity was high in group 6 (p<0.05), and enzyme activity in groups 7 and 8 was lower compared to group 6 (p<0.05), ALT enzyme activity was high in groups 7 and 8 (p<0.05), enzyme activity was higher in groups 7 and 8 compared to group 6 (p<0.05), albumin and Ca values were increased of the 3th group x (p<0.05), the Pi value was low in the 5th group (p<0.05), Pi value of the 8th group was even lower than the 6th group (p<0.05), Zn values were found to be high in the 6th group (p<0.05), and the Zn levels of the 7th and 8th groups were lower (p<0.05) compared to the 6th group. In addition to smoking, beehive air, beehive air+pollen applications were found to be interesting in that they increase cholesterol, AST enzyme activity, uric acid level, decrease GSH level, have no effect on immunoglobulins, and decrease MDA and SOD enzyme activities of bee hive air+pollen.
Açıklama
Anahtar Kelimeler
Arı kovanı havası, Biyokimyasal parametreler, Polen, Rat, Yığılca Arısı, Beehive air, Biochemical parameter, Pollen, Yığılca Bee
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Delen, S., (2022). Arı Kovanı Havası ve Polen Uygulamalarının Sigara Dumanına Maruz Bırakılan Ratlarda Canlı Ağırlık ve Bazı Biyokimyasal Parametreler Üzerine Etkilerinin Araştırılması (Doktora Tezi). Selçuk Üniversitesi, Sağlık Bilimler Enstitüsü, Konya.
