Biopotential of Bersama abyssinica fresen stem bark extracts: UHPLC profiles, antioxidant, enzyme inhibitory, and antiproliferative propensities

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dc.contributor.authorSinan, K. I.
dc.contributor.authorChiavaroli, A.
dc.contributor.authorOrlando, G.
dc.contributor.authorBene, K.
dc.contributor.authorZengin, Gökhan
dc.contributor.authorCziáky, Z.
dc.contributor.authorJek?, J.
dc.date.accessioned2020-03-26T20:20:43Z
dc.date.available2020-03-26T20:20:43Z
dc.date.issued2020
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, ethyl acetate, methanol, and water extracts of Bersama abyssinica (Melianthaceae) stem bark were screened for enzyme inhibitory and antioxidant properties. The water extract possessed the highest concentration of phenols (230.83 mg gallic acid equivalent/g extract), while the methanol extract was rich in flavonoids (75.82 mg rutin equivalent/g extract), and the ethyl acetate extract possessed the highest amount of saponins (97.37 mg quillaja equivalent/g). The aim of this study was to investigate the antiproliferative effects against the human colon cancer HCT116 cell line challenged with serotonin (5-HT) as a stimulating-proliferation factor. The level of HCT116 cell-deriving pool of kynurenic acid (KA) was also assessed. The UHPLC results confirmed the presence of 58, 68, and 63 compounds in the ethyl acetate, methanol, and water extracts, respectively. Mangiferin, vitexin and its isomer isovitexin were tentatively identified in all extracts and KA (m/z 190.05042 [M?H]+) was also tentatively identified in the methanol and water extracts. The methanol extract (1464.08 mg Trolox equivalent [TE]/g extract) showed the highest activity in the CUPRAC assay, whereas the water extract (1063.70 mg TE/g extract) showed the highest activity with the FRAP technique. The ethyl acetate extract was the most active acetylcholinesterase (4.43 mg galantamine equivalent/g extract) and ?-glucosidase (mmol acarbose equivalent /g extract) inhibitor. The water extract was able to inhibit 5-HT-stimulated viability of HCT116 cells, and blunt 5-HT-induced reduction of cell-deriving KA. The scientific data generated in this study provide baseline data regarding the biological properties of B. abyssinica stem bark, highlighting its potential use for the development of new pharmaceutic and cosmetic agents. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.en_US
dc.description.sponsorshipFAR 2019en_US
dc.description.sponsorshipFunding: The study was supported by the Italian Ministry of University funds (FAR 2019) granted to Proff. Giustino Orlando, Annalisa Chiavaroli, and Claudio Ferrante.en_US
dc.identifier.doi10.3390/antiox9020163en_US
dc.identifier.issn2076-3921en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.3390/antiox9020163
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38651
dc.identifier.volume9en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofAntioxidantsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAcetylcholinesteraseen_US
dc.subjectAfricaen_US
dc.subjectColon canceren_US
dc.subjectKynurenic aciden_US
dc.subjectMangiferinen_US
dc.subjectSerotoninen_US
dc.titleBiopotential of Bersama abyssinica fresen stem bark extracts: UHPLC profiles, antioxidant, enzyme inhibitory, and antiproliferative propensitiesen_US
dc.typeArticleen_US

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