Determination of alpha-napthyl acetate esterase activity of native and frozen-thawed bull sperm acrosome
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Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz Amaç: Akrozomal enzimlerin seminal plazmaya sızıp sızmadığının belirlenmesi, suni tohumlama için sperma işlenmesi sırasında oluşan akrozom hasarının öncü bir göstergesi olabilir. Alfa-naftil asetat esteraz enzimi bu amaçla kullanılabilecek olan akrozomal enzimlerden biridir. Bu çalışmada, taze ve dondurup-çözdürülen boğa spermatozoonlarındaki alfa-naftil asetat esteraz enziminin pozitivite oranları ve lokalizasyonunun ışık mikroskobuyla belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada materyal olarak 7 boğadan alınan sperma örneği kullanıldı. Bu amaçla, taze ve dondurulup-çözdürülen sperma örneklerinden sürme frotiler hazırlandı. Bu frotilerde alfa-naftil asetat esteraz enzimi sitokimyasal metotla demonstre edildi. Hazırlanan preparatlar ışık mikroskobuyla incelenerek alfa-naftil-asetat esteraz pozitivite oranları, enzimin lokalizasyon ve dağılım tarzı belirlendi. Bulgular: Alfa-naftil asetat esteraz enzimi pozitivitesi, spermatozoonun akrozomunda lokalize olan kırmızımsı-kahve renkli reaksiyon ürünü halinde gözlendi. Bazı hücrelerde enzimatik reaksiyon akrozom zarında dar bir band halinde gözlendi. Taze sperma örneklerinin alfa-naftil asetat esteraz pozitivitesinde belirgin bireysel farklılıklar gözlendi. Enzimatik reaksiyon, hücrelerin tamamında aynı şiddette değildi. Bazı hücrelerdeki reaksiyon nispeten daha zayıftı. Düşük spermatozoon vitalitesi ve motilitesi tesbit edilen taze sperma örneklerinin alfa-naftil asetat esteraz enzim pozitivitesi de düşüktü. Dondurup-çözme işlemi, örneklerin alfa-naftil asetat esteraz pozitivitesinde önemli düşüşe yol açtı. Öneri: Akrozomal alfa-naftil asetat esteraz enzimi, boğa spermasının fertilite potansiyelinin ve dondurma işlemine uygunluğunun değerlendirilmesinde ve önemli bir parametre olarak dikkate alınabileceği sonucuna varılmıştır.
Aim: The assay of the leakage of acrosomal enzymes into seminal plasma might provide an early and sensitive evidence for membrane damage ocurred during freezing of semen for artificial insemination. Alpha-naphthyl acetate esterase is one of the acrosomal enzymes that can be used for this purpose. In the the present study, the positivity rates and localization pattern of alphanaphthyl acetate esterase in the spermatozoa of both native and frozen-thawed bull semen samples were determined by light microscopy. Materials and Methods: Semen samples of 7 bull were used as material. Smears were prepared from native and frozen-thawed semen samples. The enzyme was demonstrated by a cytochemical method. Specimens were observed under ligth microscope. Positivity rates of alpha-naphtyl acetate eseterasepositive spermatozoa, distribution and localization pattern of the enzymatic reaction were determined. Results: The reddish-brown enzymatic reaction was peculiar to spermatozoon acrosome. In some cells, the enzymatic reaction was observed as a narrow band located on acrosomal membrane. The enzymatic reaction intesity was not similar in all cells and was weaker in some cells. The reaction product primarily located in the acrosome. There were large individual differences in alpha-naphthyl acetate esterase positivity rates of the native sperm samples. The native semen samples with low spermatozoon vitality and motility tended to display lower of alpha-naphthyl acetate esterase positivity rates. Freeze-thawing process significantly decreased alpha-naphthyl acetate esterase positivity rates of the samples. Conclusion: Acrosomal alpha-naphtyl acetate esterase might be considered as a significant parameter in the asssessment of availibility for freezing and to determine fertility potential of the bull semen.
Aim: The assay of the leakage of acrosomal enzymes into seminal plasma might provide an early and sensitive evidence for membrane damage ocurred during freezing of semen for artificial insemination. Alpha-naphthyl acetate esterase is one of the acrosomal enzymes that can be used for this purpose. In the the present study, the positivity rates and localization pattern of alphanaphthyl acetate esterase in the spermatozoa of both native and frozen-thawed bull semen samples were determined by light microscopy. Materials and Methods: Semen samples of 7 bull were used as material. Smears were prepared from native and frozen-thawed semen samples. The enzyme was demonstrated by a cytochemical method. Specimens were observed under ligth microscope. Positivity rates of alpha-naphtyl acetate eseterasepositive spermatozoa, distribution and localization pattern of the enzymatic reaction were determined. Results: The reddish-brown enzymatic reaction was peculiar to spermatozoon acrosome. In some cells, the enzymatic reaction was observed as a narrow band located on acrosomal membrane. The enzymatic reaction intesity was not similar in all cells and was weaker in some cells. The reaction product primarily located in the acrosome. There were large individual differences in alpha-naphthyl acetate esterase positivity rates of the native sperm samples. The native semen samples with low spermatozoon vitality and motility tended to display lower of alpha-naphthyl acetate esterase positivity rates. Freeze-thawing process significantly decreased alpha-naphthyl acetate esterase positivity rates of the samples. Conclusion: Acrosomal alpha-naphtyl acetate esterase might be considered as a significant parameter in the asssessment of availibility for freezing and to determine fertility potential of the bull semen.
Açıklama
Anahtar Kelimeler
Boğa spermatozoonları, akrozomal enzimler, dondurup çözdürme, alfa-naftil asetat esteraz, Bull spermatozoa, acrosomal enzymes, freeze-thaw
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
1
Künye
Canbolat, Ü. N., Çelik, İ., Erdoğan, R. (2020). Determination of alpha-napthyl acetate esterase activity of native and frozen-thawed
bull sperm acrosome. Eurasian Journal of Veterinary Sciences, 36 (1), 1-7. DOI: 10.15312/EurasianJVetSci.2020.252
