Streptozotosin ile diyabet oluşturulmuş deneysel periodontitisli sıçanlarda insülin ve alfa-tokoferol tedavisinin serum sitokin düzeyleri ve dişeti iNOS ve CD95 ekspresyonu üzerine etkileri
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Dosyalar
Tarih
2011
Yazarlar
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Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu araştırmanın amacı, deneysel periodontitis ve streptozotosin (STZ) ile diyabet oluşturulan/oluşturulmayan sıçanlarda ? -tokoferol ve/veya insülin'in alveoler kemik kaybı, dişeti iNOS ve CD95 + hücre sayılarına etkilerini araştırmaktır. Toplam 60 adet Sprague Dawley sıçan üç gruba ayrıldı: Grup I: Diyabetsiz grup, Grup II: STZ ile diyabet oluşturulan grup, Grup III: STZ ile diyabet oluşturulan ve insülin terapisi verilen grup (4 U/gün). Tüm hayvanlara anestezi yapıldı ve 3/0 ipek sütur mandibular alt molar dişlerin çevresine yerleştirildi. Tüm gruplar serum fizyolojik (Grup IA, Grup IIA, Grup IIIA) ve ? -tokoferol (40mg/kg/gün) enjekte edilen gruplara (Grup IB, Grup IIB, Grup IIIB) ayrıldı. Üç haftalık süre sonunda tüm sıçanlar kurban edildi ve dokular histometrik ve immünohistokimyasal ölçümler için nötral tamponlanmış %10'luk formaldehit solüsyonunda sabitlendi. Alveoler kemik kaybı (AKK), dişeti iNOS+ ve CD95+ hücre sayıları görüntü analiz programı kullanılarak analiz edildi. Serum IL-1 ? , IL-4 ve IL-6 konsantrasyonları ticari ELISA kitleri kullanılarak ölçüldü. İstatistiksel kıyaslamalar için iki-yönlü varyans analizi, tek-yönlü varyans analizi ve Tukey HSD kullanıldı p<0,05 anlamlılık düzeyi kabul edildi. Çalışmanın sonunda gruplar arasında AKK ve serum sitokin konsantrasyonları farklı bulunmadı (p>0,05). Alfa-tokoferol uygulaması Grup IB, Grup IIB ve Grup IIIB'deki dişeti iNOS+ ve CD95+ hücre sayılarını, serum fizyolojik (Grup IB, Grup IIB ve Grup IIIB) gruplarına kıyasla baskıladı (p<0,05). Grup IIA'daki sıçanlar Grup IA ve Grup IIIA'ya göre daha fazla düzeyde iNOS+ ve CD95+ hücre sayısı gösterdi (p<0,05). Grup IB ve Grup IIIB'deki dişeti iNOS+ ve CD95+ hücre sayıları benzer bulundu (p>0,05). Bu araştırmanın sınırları içerisinde, ? -tokoferol'ün deneysel periodontitis oluşturulmuş STZ ile diyabet oluşturulmuş/oluşturulmamış sıçanlarda dişetindeki oksidatif hasarı ve apoptotik hücre ölümünü azalttığı ve ayrıca insülinin etkinliğini arttırdığı ileri sürülebilir.
The aim of this study was to investigate the effects of ? -tocopherol and/or insulin administration on alveolar bone loss, number of inductible nitric oxide synthase (iNOS)+, CD95+ cells in gingival tissues and serum cytokine concentrations in rats with experimental periodontitis with/without streptozotocin (STZ)-induced diabetes. Sixty male Sprague Dawley rats were divided into three groups: Group I: non-diabetic group, Group II: STZ-induced type I diabetes, Group III: STZ induced type I diabetes and insulin therapy (4 U/day). All animals were anesthetized, and 3/0 silk sutures were placed at the gingival margin level of the mandibular right first molars. All groups divided into two subgroups; thirty rats was injected with saline (Group IA, Group IIA, Group IIIA) and other thirty rats (Group IB, Group IIB, Group IIIB) was injected with ? -tocopherol (40mg/kg/day). After 3 weeks, all rats were sacrified and the tissues were fixed using neutral buffered %10 formaldehyde solution for histometrical and immunohistochemical investigation. Alveolar bone loss (ABL), number of iNOS+ and CD95+ cells in gingival tissues were analyzed by using image analysis program. The concentrations of serum IL-1 ? , IL-4 and IL-6 levels were measured by using commercial ELISA kits. Statistical comparisons were performed by using two-way analysis of variance, one way analysis of variance and Tukey HSD, with significance set at p<0,05. At the end of the study, there were no statistically significant differences in ABL and serum cytokine concentrations between the study groups (p ? 0,05). Administration of ? -tocopherol were suppressed number of iNOS and CD95+ cells in gingival tissues in Group IB, Group IIB, Group IIIB rats, compared to saline groups (Group IA, Group IIA, Group IIIA) (p<0,05). Rats from group IIA showed significantly higher levels in number of iNOS+ and CD95+ cells compared with rats from Group IA and Group IIIA (p<0,05). The number of iNOS and CD95+ cells in gingival tissues were similar levels in Group IB and group IIIB (p ? 0,05). Within the limits of this study, it can be suggested that ? -tocopherol decreases oxidative damage and apoptotic cell death in gingival tissues of rats with experimental periodontitis with/without STZ-induced diabetes and also improves the action of insulin.
The aim of this study was to investigate the effects of ? -tocopherol and/or insulin administration on alveolar bone loss, number of inductible nitric oxide synthase (iNOS)+, CD95+ cells in gingival tissues and serum cytokine concentrations in rats with experimental periodontitis with/without streptozotocin (STZ)-induced diabetes. Sixty male Sprague Dawley rats were divided into three groups: Group I: non-diabetic group, Group II: STZ-induced type I diabetes, Group III: STZ induced type I diabetes and insulin therapy (4 U/day). All animals were anesthetized, and 3/0 silk sutures were placed at the gingival margin level of the mandibular right first molars. All groups divided into two subgroups; thirty rats was injected with saline (Group IA, Group IIA, Group IIIA) and other thirty rats (Group IB, Group IIB, Group IIIB) was injected with ? -tocopherol (40mg/kg/day). After 3 weeks, all rats were sacrified and the tissues were fixed using neutral buffered %10 formaldehyde solution for histometrical and immunohistochemical investigation. Alveolar bone loss (ABL), number of iNOS+ and CD95+ cells in gingival tissues were analyzed by using image analysis program. The concentrations of serum IL-1 ? , IL-4 and IL-6 levels were measured by using commercial ELISA kits. Statistical comparisons were performed by using two-way analysis of variance, one way analysis of variance and Tukey HSD, with significance set at p<0,05. At the end of the study, there were no statistically significant differences in ABL and serum cytokine concentrations between the study groups (p ? 0,05). Administration of ? -tocopherol were suppressed number of iNOS and CD95+ cells in gingival tissues in Group IB, Group IIB, Group IIIB rats, compared to saline groups (Group IA, Group IIA, Group IIIA) (p<0,05). Rats from group IIA showed significantly higher levels in number of iNOS+ and CD95+ cells compared with rats from Group IA and Group IIIA (p<0,05). The number of iNOS and CD95+ cells in gingival tissues were similar levels in Group IB and group IIIB (p ? 0,05). Within the limits of this study, it can be suggested that ? -tocopherol decreases oxidative damage and apoptotic cell death in gingival tissues of rats with experimental periodontitis with/without STZ-induced diabetes and also improves the action of insulin.
Açıklama
Anahtar Kelimeler
Insulin, İnsülin, Tocopherol, Tokoferol, Rats, Sıçanlar, Streptozotocin, Streptozotocin, Cytokines, Sitokinler, Periodontitis, Periodontit, Periodontal diseases, Periodontal hastalıklar, Nitric oxide, Nitrik oksit, Gingiva, Diabetes mellitus
Kaynak
WoS Q Değeri
Scopus Q Değeri
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Künye
Hatipoğlu, M. (2011). Streptozotosin ile diyabet oluşturulmuş deneysel periodontitisli sıçanlarda insülin ve alfa-tokoferol tedavisinin serum sitokin düzeyleri ve dişeti iNOS ve CD95 ekspresyonu üzerine etkileri. Selçuk Üniversitesi, Yayımlanmış doktora tezi, Konya.
