Characterization of Consolida Sf Gray (Ranunculaceae) Taxa in Turkey by Seed Storage Protein Electrophoresis

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dc.contributor.authorErtuğrul, Kuddisi
dc.contributor.authorArslan, Emine
dc.contributor.authorTugay, Osman
dc.date.accessioned2020-03-26T17:47:23Z
dc.date.available2020-03-26T17:47:23Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective: This research aimed to investigate the genetic relations of Consolida S. F. Gray species collected in Turkey using seed storage proteins. This study has become significant as the taxonomic status of genus Consolida changes often. Methods: Mature seeds of 22 Consolida species were collected. Total protein were isolated from seeds collected for each species. Sodium Dodecyl Sulphate-Polycarylamide Gel Electrophoresis was performed by a standard method on a vertical slab gel. After electrophoresis, the protein bands were visualized by staining with Coomassie Brillant Blue G-250.The polymorphic bands were scored visually as present (1) or absent (0). Genetic similarity among taxa was estimated basing on Nei's homology. Results: In all, 74 polypeptide bands of different sizes ranging from 25 to 140 kDa were observed in the 22 taxa of the genus Consolida. Cluster analysis was performed using the unweighted pair-group method and arithmetic averages in Bio1D++ computer program. According to similarity coefficients, the genus Consolida was separated into the main two groups. It was discovered that the first group consisted of the species of Consolida with 5-lobed corolla, and in the second main group were the species with 3-lobed corolla. Conclusion: It is concluded that seed storage protein profiles could be useful markers in the studies of genetic relations of Consolida species. The grouping as a result of the dendogram has also supported the relationship in Flora of Turkey.en_US
dc.identifier.citationErtuğrul, K., Arslan, E., Tugay, O., (2010). Characterization of Consolida Sf Gray (Ranunculaceae) Taxa in Turkey by Seed Storage Protein Electrophoresis. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi, 35(2), 97-102.
dc.identifier.endpage102en_US
dc.identifier.issn0250-4685en_US
dc.identifier.issn1303-829Xen_US
dc.identifier.issue2en_US
dc.identifier.startpage97en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24686
dc.identifier.volume35en_US
dc.identifier.wosWOS:000278764100004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorErtuğrul, Kuddisi
dc.institutionauthorArslan, Emine
dc.institutionauthorTugay, Osman
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectConsolidaen_US
dc.subjectSds-pageen_US
dc.subjectGenetic relationen_US
dc.subjectTotal proteinsen_US
dc.titleCharacterization of Consolida Sf Gray (Ranunculaceae) Taxa in Turkey by Seed Storage Protein Electrophoresisen_US
dc.typeArticleen_US

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