Potential Therapeutic Effect of Thymoquinone on Cisplatin-induced Hepatotoxicity in Rats
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Dosyalar
Tarih
2024 Mart
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz Amaç: Antineoplastik bir ajan olan Sisplatin (CIS), hepatotoksisite gibi ciddi yan etkiler gösterir. Timokinon (ThQ), anti-inflamatuar, anti-tümöral gibi birçok faydalı etkiye sahip güçlü bir anti-oksidandır. Bu çalışmanın amacı, sıçanlarda CIS kaynaklı hepatotoksisite modelinde ThQ tedavisinin hepatoprotektif etkisinin olup olmadığının incelenmesi ve ayrıca CIS ve\veya ThQ uygulamalarının karaciğer dokusunda, deubikuitinazlardan biri olan OTULIN düzeylerini nasıl etkilendiğinin belirlenmesidir. Gereç ve Yöntem: Çalışmada kullanılan 28 adet rat; kontrol (uygulama yok), CIS (7 mg/kg CIS, deneyin ilk günü, intraperitoneal), CIS+ThQ (7 mg/kg CIS, deneyin ilk günü, intraperitoneal + 10 mg/kg/gün ThQ, oral gavaj) ve ThQ (10 mg/kg/gün ThQ, oral gavaj) olmak üzere eşit sayıda dört gruba ayrıldı (n=7). Tüm uygulamaların tamamlanmasıyla (15. gün) deney sonlandırıldı. CIS ve\ veya ThQ uygulamalarının karaciğer dokusu üzerindeki etkileri biyokimyasal, histopatolojik ve immünohistokimyasal olarak incelendi. Bulgular: CIS uygulaması karaciğer dokusunda karaciğer enzim düzeylerini, histopatolojik değişiklikleri, oksidatif stresi, inflamasyonu ve apoptozu arttırırken OTULIN düzeyinin azalmasına neden olmuştur. Bununla birlikte ThQ uygulaması, karaciğer dokusunda CIS kaynaklı oluşan değişiklikleri olumlu yönde düzenleyici etki göstermiştir. Öneri: ThQ takviyesi CIS kaynaklı karaciğer dokusu hasarına karşı hepotoprotektif etkiye sahiptir.
Abstract Aim: Cisplatin (CIS), an antineoplastic agent, shows serious side effects such as hepatotoxicity. Thymoquinone (ThQ) is a powerful anti-oxidant with many beneficial effects such as anti-inflammatory, anti-tumoral. The aim of this study was to investigate whether ThQ treatment has hepatoprotective effect in CIS-induced hepatotoxicity model in rats and also to determine how CIS and/or ThQ treatments affect OTULIN levels, one of the deubiquitinases, in liver tissue. Materials and Methods: The 28 rats used in the study control (application not done), CIS (7 mg/kg CIS, first day of experiment, intraperitoneal), CIS+ThQ (7 mg/kg CIS, first day of experiment, intraperitoneal + 10 mg/kg/day ThQ, oral gavage) and ThQ (10 mg/kg/day ThQ, oral gavage) were divided into four equal groups (n=7). The experiment was terminated upon completion of all applications (day 15). The effects of CIS and\or ThQ apps on liver tissues were examined biochemically, histopathologically and immunohistochemically. Results: CIS application increased liver enzyme levels, histopathological changes, inflammation, oxidative stress and apoptosis in liver tissue and caused a decrease in OTULIN level. However, ThQ administration showed a positive regulatory effect on CIS-induced changes in liver tissue.. Conclusion: ThQ supplementation has a hepatoprotective effect against CISinduced liver tissue damage.
Abstract Aim: Cisplatin (CIS), an antineoplastic agent, shows serious side effects such as hepatotoxicity. Thymoquinone (ThQ) is a powerful anti-oxidant with many beneficial effects such as anti-inflammatory, anti-tumoral. The aim of this study was to investigate whether ThQ treatment has hepatoprotective effect in CIS-induced hepatotoxicity model in rats and also to determine how CIS and/or ThQ treatments affect OTULIN levels, one of the deubiquitinases, in liver tissue. Materials and Methods: The 28 rats used in the study control (application not done), CIS (7 mg/kg CIS, first day of experiment, intraperitoneal), CIS+ThQ (7 mg/kg CIS, first day of experiment, intraperitoneal + 10 mg/kg/day ThQ, oral gavage) and ThQ (10 mg/kg/day ThQ, oral gavage) were divided into four equal groups (n=7). The experiment was terminated upon completion of all applications (day 15). The effects of CIS and\or ThQ apps on liver tissues were examined biochemically, histopathologically and immunohistochemically. Results: CIS application increased liver enzyme levels, histopathological changes, inflammation, oxidative stress and apoptosis in liver tissue and caused a decrease in OTULIN level. However, ThQ administration showed a positive regulatory effect on CIS-induced changes in liver tissue.. Conclusion: ThQ supplementation has a hepatoprotective effect against CISinduced liver tissue damage.
Açıklama
Anahtar Kelimeler
Hepototoksisite, otulin, sisplatin, timokinon, Hepatotoxicity, cisplatin, thymoquinone
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
40
Sayı
1
Künye
Kaya, S., Yalçın, T., (2024). Potential Therapeutic Effect of Thymoquinone on Cisplatin-induced Hepatotoxicity in Rats. Eurasian Journal of Veterinary Sciences, 40(1), 1-9. DOI: 10.15312/EurasianJVetSci.2024.419
