Erythrocyte and spermatozoa glucose-6-phosphate dehydrogenase activity in merino rams: An experimental study

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dc.contributor.authorGürel, Hüseyin
dc.contributor.authorBaşpınar, Nuri
dc.contributor.authorAkalın, Pınar Peker
dc.contributor.authorAltunok, Vahdettin
dc.contributor.authorKazak, Filiz
dc.date.accessioned2020-03-26T20:11:46Z
dc.date.available2020-03-26T20:11:46Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (×106), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p?0.001), whereas spermatozoa G6PD activity was lower (p?0.001) in the breeding season (1.928±0.231 U/g hemoglobin, 129.65±28.41 U/g protein, respectively) from that in the non-breeding (0.530±0.066 U/g hemoglobin, 562.36±94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance. © 2018, Research and Clinical Center for Infertitlity. All rights reserved.en_US
dc.identifier.endpage378en_US
dc.identifier.issn2476-4108en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage373en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37196
dc.identifier.volume16en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherResearch and Clinical Center for Infertitlityen_US
dc.relation.ispartofInternational Journal of Reproductive BioMedicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectErythrocyteen_US
dc.subjectGlucose-6-phosphate dehydrogenaseen_US
dc.subjectRamen_US
dc.subjectSpermatozoaen_US
dc.titleErythrocyte and spermatozoa glucose-6-phosphate dehydrogenase activity in merino rams: An experimental studyen_US
dc.typeArticleen_US

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