The effects of medical ozone in rat heart exposed to ischemia-reperfusion injury: Experimental study

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Objective: In this study, it was intended to investigate the effects of preconditioning with medical ozone on possible reperfusion injury, in which patients undergoing the operation due to coronary artery disease. Material and Methods: 37 female mature rats were categorized into 4 groups either as sham-control (Group I), ischemia (Group II), ischemic preconditioning by oxygen (Group III) or by medical ozone (Group IV). Through left thoracotomy, rats were subjected to 25 min of ischemia on left anterior descending (LAD) coronary artery and after that, reperfusion was provided for 75 min. At the end of this period, serum sampling for creatinine-kinase (CK)-MB, Troponin-I, superoxide dismutase (SOD) and malondialdehyde (MDA) and tissue sampling for histopathological examinations were performed. The results of pathological examination were divided into 3 groups according to cellular damage. Results: Statistically, there were no significant differences between the groups in case of CK-MB levels and SOD enzymatic activity (p>0.05). Nevertheless, Troponin-I was higher in Group III and MDA was meaningfully lower in Group IV in respect to control group (p=0.005). Moreover, MDA levels were significantly lower in Group IV, when compared to Group III. With these results, no significant differences were detected between ischemia and ozone group. Although it is important to detect lower MDA levels in ozone group when compared to oxygen group, it is hard to talk about protective effects of ozone just depending on this data. In histopathological examination, medical ozone had beneficial effects on cellular protection when compared to ischemic group; but when the groups were examined, no significant differences could be found between oxygen and ozone groups. Conclusion: Although the role of medical ozone on the prevention of reperfusion injury could not be demonstrated clearly, it might have some beneficial effects after biochemical and histopathological evaluation. To be able to show these effects precisely, more studies are needed. Copyright © 2012 by Türkiye Klinikleri.


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Ischemic preconditioning, Ozone, Reperfusion injury


Turkiye Klinikleri Cardiovascular Sciences

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