Sporda doping amaçlı kullanılan nandrolonun puberta dönemindeki ratların Femur ve Humerus'u üzerine morfometrik etkisi
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Dosyalar
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu araştırmanın amacı sporda doping amaçlı kullanılan nandrolonun puberta dönemindeki ratların femur ve humerus'u üzerine morfometrik etkisini belirlemektir. Araştırmada 30 günlük 60 adet Spraque Dawley rat kullanıldı. Deney uygulamaları Selçuk Üniversitesi Veteriner Fakültesi Deney Hayvanları Ünitesinde gerçekleştirildi. Çalışma protokolü aynı fakültenin etik kurulu tarafından onandı. Erkek ve dişi ratlar nandrolon, fıstık yağı ve kontrol olarak üç farklı gruba ayrıldı. Nandrolon 10 mg/kg dozunda ticari ilacın dilüenti olan fıstık yağında intraperitoneal yolla 5 gün uygulanıp 2 gün dinlendirilerek 4 hafta uygulandı (Nandrolon grubu). Fıstık yağı grubuna fıstık yağı intraperitoneal olarak nandrolon grubunda olduğu gibi uygulandı. Kontrol grubuna hiçbir uygulama yapılmadı. Ratlar 4. hafta sonunda pentobarbitalin intraperitoneal enjeksiyon ile ötenazi edildi. Daha sonra materyallerin ön ve arka extremiteleri ortaya çıkarılarak diseke edildi. Sağ yüzdeki humerus ve femur kemiklerinin belirlenen anatomik referans noktalarından her birine gerekli morfometrik ölçümler kumpasla yapıldı. Nandrolon, fıstık yağı ve kontrol gruplarının femur kemiği boy uzunlukları ortalamaları arasındaki fark istatistiksel olarak önemli bulundu (p<0,05). Dişi nandrolon ile dişi fıstık yağı ve kontrol gruplarının humerus boy uzunlukları ortalamaları arasındaki fark istatistiksel olarak önemli bulundu (p<0,05). Nandrolon grubunun femur ve humerus kemiklerinin boy uzunlukları arasında ise cinsiyet açısından aralarında anlamlı bir farklılık bulunmadı (p>0,05). Dişi nandrolon grubunun humerus cortex kalınlığı ve medullar çapı istatistikî olarak diğer gruplarda farklıydı (p<0,05). Sonuç olarak nandrolonun özellikle puberta dönemindeki sporcular başta olmak üzere tüm sporcularda kullanılması femur ve humerus gibi kemiklerin uzamasını durdurabileceği kanaatine varıldı.
The purpose of this research was to evaluate the morphometric effects on nandrolone used as doping in sports on femur and humerus of rats in the pubertal term. 30-day, 60 pieces Spraque Dawley rats were used in the study. Experimental applications were performed in Laboratory Animal Unit of Selcuk University, Veterinary Faculty. The study protocol was approved by the ethics committee of the same faculty. Male and female rats were divided into three different groups as nandrolone, peanut oil, and control groups. Nandrolone was injected 10 mg / kg intraperitoneally within peanut oil diluents of the commercial drug during 4 weeks, as 5 days and left for 2 days of rest by the break (Nandrolone group). Peanut oil group was applied penult oil via intraperitoneal like nandrolone group. The control group was no any treat. Rats were got euthanasia with intraperitoneal injection of pentobarbital at the end of the fourth week. And then, front and rear extremities of the material by being revealed were dissected. Necessary morphometric measurements were made with calipers to every one of the anatomical reference points determined of the humerus and femur bones on the right side. Statistically significance (p<0.05) difference was determined amongst femur bone height averages of nandrolone, peanut oil and control groups. Difference between humerus height averages of female penult group, female control groups and female nandrolone group was found statistically significant (p<0,05). There was no statistically significance determined between the height of the femur and humerus bones of the nandrolone group (p>0,05), compared to sex difference. Cortex thickness and medullar diameter of nandrolone female humerus were statistically significant than other groups (p<0.05). As a result, it can be concluded that nandrolon, used mainly athletes in the puberty period and in all athletes, stops elongation of the bones such as femur and humerus.
The purpose of this research was to evaluate the morphometric effects on nandrolone used as doping in sports on femur and humerus of rats in the pubertal term. 30-day, 60 pieces Spraque Dawley rats were used in the study. Experimental applications were performed in Laboratory Animal Unit of Selcuk University, Veterinary Faculty. The study protocol was approved by the ethics committee of the same faculty. Male and female rats were divided into three different groups as nandrolone, peanut oil, and control groups. Nandrolone was injected 10 mg / kg intraperitoneally within peanut oil diluents of the commercial drug during 4 weeks, as 5 days and left for 2 days of rest by the break (Nandrolone group). Peanut oil group was applied penult oil via intraperitoneal like nandrolone group. The control group was no any treat. Rats were got euthanasia with intraperitoneal injection of pentobarbital at the end of the fourth week. And then, front and rear extremities of the material by being revealed were dissected. Necessary morphometric measurements were made with calipers to every one of the anatomical reference points determined of the humerus and femur bones on the right side. Statistically significance (p<0.05) difference was determined amongst femur bone height averages of nandrolone, peanut oil and control groups. Difference between humerus height averages of female penult group, female control groups and female nandrolone group was found statistically significant (p<0,05). There was no statistically significance determined between the height of the femur and humerus bones of the nandrolone group (p>0,05), compared to sex difference. Cortex thickness and medullar diameter of nandrolone female humerus were statistically significant than other groups (p<0.05). As a result, it can be concluded that nandrolon, used mainly athletes in the puberty period and in all athletes, stops elongation of the bones such as femur and humerus.
Açıklama
Anahtar Kelimeler
Doping, Drug, Femur, Humerus, Nandrolone, Sıçanlar, Rats
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Lök, S. (2009). Sporda doping amaçlı kullanılan nandrolonun puberta dönemindeki ratların Femur ve Humerus'u üzerine morfometrik etkisi. Selçuk Üniversitesi, Yayımlanmış doktora tezi, Konya.
