Ereğli (Konya, Türkiye) Bölgesinden Bal Arılarının Topladığı Polenin Etanol Ekstraktının Antioksidan ve in Vitro Bazı Enzim İnhibitör Aktiviteleri
Yükleniyor...
Dosyalar
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Fen Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Arı poleni bal arıları tarafından granüller şeklinde paketlenen ve daha sonra insanlar tarafından kovanlardan hasat edilen en saf ve en zengin doğal besin takviyelerinden biridir. Bal arılarının topladığı polen, proteinler, aminoasitler, karbohidratlar, lipidler, vitaminler, mineral maddeler gibi besleyici temel unsurlardan oluşur. Polenin sağlık üzerindeki olumlu etkisi fenolik bileşiklere sahip olmasından dolayı antioksidan aktivitesine bağlanmaktadır. Son yıllarda arı poleniyle ilgili çok sayıda araştırma yayınlanmıştır. Arı poleni ve diğer arı ürünleri üzerinde birçok araştırma yapılmış olsa da, enzim inhibisyonu üzerine çok az çalışma bulunmaktadır. Bu nedenle, bu araştırmanın amacı Ereğli (Konya, Türkiye) bölgesinden bal arılarının topladığı karışık polenin etanol ekstraktının total fenolik ve flavonoid içeriklerini ve antioksidan aktivitesini, ayrıca bu ekstraktın kolinesteraz, amilaz ve glukozidaza karşı enzim inhibitör potansiyellerini ilk kez tespit etmektir. Bu çalışmada rutin çalışmalardan farklı olarak ultrasonik ekstraksiyon yöntemi kullanıldı. Ekstrakttaki total fenolik ve flavonoid içerikleri de Folin-Ciocalteu ve AlCl3 yöntemleriyle tespit edildi. Antioksidan aktiviteleri serbest radikal süpürme (DPPH ve ABTS), indirgeme gücü (FRAP ve CUPRAC), fosfomolibdat ve metal şelatlama testleri gibi farklı yöntemlerle araştırıldı. In vitro enzim inhibitör potansiyelleri bir mikroplate okuyucuyla ölçüldü. Aktiviteler standart eş değerler olarak değerlendirildi. Bu çalışmanın sonuçları göstermiştir ki, i. antioksidan aktivitesi açısından, Ereğli bölgesinden elde edilen arı poleni sağlığa yararlı formülasyonlarda ileride kullanmak için değerli fonksiyonel içeriğiyle doğal bir kaynak olarak düşünülebilir, ii. bu bölgenin poleni, Alzheimer ve Diabetes mellitus gibi önemli sağlık problemlerinin tedavisi için doğal bir enzim inhibitörü kaynağı olarak göz önüne alınabilir
Pollen of bee is one of the purest and the richest natural food supplements packed by honeybees into granules and subsequently harvested from hives by humans. Honeybee-collected pollen is composed of nutritionally essential substances such as proteins, amino acids, carbohydrates, lipids, vitamins, mineral substances and trace elements. Its beneficial effect on health is thought to be due to the presence of phenolic compounds with its antioxidant activity. In recent years, many papers have been published on issues concerning bee pollen. Although many studies have been conducted on bee pollen and other bee products, there are few studies of enzyme inhibition. Therefore, the aim of this study was to determine the total phenolic and flavonoid contents and antioxidant activity of ethanol extract of honeybee-collected mixed pollen from Ereğli (Konya, Turkey) region, as well as the enzyme inhibitory potentials of this extract against cholinesterase, amylase and glucosidase for the first time. In the present study, ultrasonication assisted extraction method was used in contrast to routine methods. Total phenolic and flavonoid contents present in the extract were also determined by Folin-Ciocalteu and AlCl3 assays. Antioxidant activities were investigated by using different assays, including free radical scavenging assays (DPPH and ABTS) reducing power (FRAP and CUPRAC), phosphomolybdenum and metal chelating assays. The in vitro enzyme inhibitory potentials were measured with a microplate reader. The activities were evaluated as standard equivalents. Results indicate that, i. in terms of antioxidant activity, pollen of bee obtained from Ereğli region could be considered as a natural source of valued functional ingredients for further use in healthful formulations, ii. this pollen could be taken into consideration as a source of natural enzyme inhibitors for the treatment of major health problems such as Alzheimer disease and Diabetes mellitus
Pollen of bee is one of the purest and the richest natural food supplements packed by honeybees into granules and subsequently harvested from hives by humans. Honeybee-collected pollen is composed of nutritionally essential substances such as proteins, amino acids, carbohydrates, lipids, vitamins, mineral substances and trace elements. Its beneficial effect on health is thought to be due to the presence of phenolic compounds with its antioxidant activity. In recent years, many papers have been published on issues concerning bee pollen. Although many studies have been conducted on bee pollen and other bee products, there are few studies of enzyme inhibition. Therefore, the aim of this study was to determine the total phenolic and flavonoid contents and antioxidant activity of ethanol extract of honeybee-collected mixed pollen from Ereğli (Konya, Turkey) region, as well as the enzyme inhibitory potentials of this extract against cholinesterase, amylase and glucosidase for the first time. In the present study, ultrasonication assisted extraction method was used in contrast to routine methods. Total phenolic and flavonoid contents present in the extract were also determined by Folin-Ciocalteu and AlCl3 assays. Antioxidant activities were investigated by using different assays, including free radical scavenging assays (DPPH and ABTS) reducing power (FRAP and CUPRAC), phosphomolybdenum and metal chelating assays. The in vitro enzyme inhibitory potentials were measured with a microplate reader. The activities were evaluated as standard equivalents. Results indicate that, i. in terms of antioxidant activity, pollen of bee obtained from Ereğli region could be considered as a natural source of valued functional ingredients for further use in healthful formulations, ii. this pollen could be taken into consideration as a source of natural enzyme inhibitors for the treatment of major health problems such as Alzheimer disease and Diabetes mellitus
Açıklama
Anahtar Kelimeler
Antioksidan, Arı poleni, Enzim inhibisyonu, Antioxidant, Bee pollen, Enzyme inhibition
Kaynak
Selçuk Üniversitesi Fen Fakültesi Fen Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
43
Sayı
2
Künye
Özparlak, H., Zengi̇n, G., Ceylan, R. (2017). Ereğli (Konya, Türkiye) bölgesinden bal arılarının topladığı polenin etanol ekstraktının antioksidan ve in vitro bazı enzim inhibitör aktiviteleri. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, 43, (2), 163-173.
