Ratlarda mekanik ventilasyona bağlı akciğer hasarının önlenmesinde kafeik asidin etkisi
Küçük Resim Yok
Tarih
2012
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info:eu-repo/semantics/openAccess
Özet
Amaç: Ventilatörle ilişkili akciğer hasarı (ventilation induced lung injury, VILI), mekanik ventilasyondaki (MV) hastaların tedavisinde önemli bir sorun oluşturmaktadır. Kafeik asit (KA) immünomodülatör, antiproliferatif, antiinflamatuar ve antioksidan özellikte olan bir moleküldür. Bu çalışmada, MV’ye bağlı olarak gelişen VILI’nın önlenmesinde KA’nın etkisi araştırıldı. Gereç ve Yöntemler: Çalışmaya alınan ratlar 4 gruba ayrıldı. Grup 1’e (kontrol grubu) fizyolojik volümlerde (9 mL/kg) MV, Grup 2’ye aşırı doz (35 mL/kg) MV, Grup 3’e aşırı doz MV ve 10 ?mol/kg KA, Grup 4’e ise aşırı doz MV ve 30 ?mol/kg KA uygulandı. Tüm gruplarda akciğer hasarının göstergesi olabilecek değişiklikler histopatolojik olarak değerlendirildi. Bulgular: Kontrol grubu ile karşılaştırıldığında, Grup 2 ratlarda akciğer hasarı göstergelerinde artış tespit edildi (p0,01). Diğer yandan KA uygulanan Grup 3’te Grup 2 örneklerine göre akciğer hasarı bulgularının çok daha düşük şiddette ortaya çıktığı görüldü (p0,01). KA uygulanan Grup 3 ile 4 ratlarda belirlenen akciğer hasarı histopatolojik bulgularının şiddeti arasında ise anlamlı fark yoktu. Sonuç: Yüksek tidal volümlerin VILI’ya sebep olduğunu ve KA’nın bunu önlediğini histopatolojik olarak gösterdik. -
Aim: entilation induced lung injury (VILI) constitutes a serious problem in the treatment of patients on mechanical ventilation (MV). Caffeic acid (CA) is a molecule with immunomodulating, antiproliferative, antiinflammatory and antioxidant properties. In this study the effect of CA in preventing VILI caused by MV has been investigated. Materials and Methods: The rats included in the study were divided into four groups. Group 1, designated as the control group, was subjected to physiological volumes (9 ml/kg) of MV; Group 2 was subjected to overdose (35 ml/kg) of MV; Group 3 received an overdose of MV and 10 &#956;mol/kg of CA; and Group 4 received an overdose of MV and 30 10 &#956;mol/kg of Ca. In all four groups the changes indicating lung damage were estimated histopathologically. Results: An increase in lung damage was observed in Group 2 rats as compared to the control Group 1 (p<0.01), whereas much less lung damage was noted in the Group 3 rats as compared to the Group 2 rats (p<0.01). There was no statistically significant difference in the severity of histopathologically estimated lung damage between the rats of Group 3 and Group 4. Conclusion: We have shown through histopathological indices that high tidal volumes of ventilation cause VILI and that CA can prevent these.
Aim: entilation induced lung injury (VILI) constitutes a serious problem in the treatment of patients on mechanical ventilation (MV). Caffeic acid (CA) is a molecule with immunomodulating, antiproliferative, antiinflammatory and antioxidant properties. In this study the effect of CA in preventing VILI caused by MV has been investigated. Materials and Methods: The rats included in the study were divided into four groups. Group 1, designated as the control group, was subjected to physiological volumes (9 ml/kg) of MV; Group 2 was subjected to overdose (35 ml/kg) of MV; Group 3 received an overdose of MV and 10 &#956;mol/kg of CA; and Group 4 received an overdose of MV and 30 10 &#956;mol/kg of Ca. In all four groups the changes indicating lung damage were estimated histopathologically. Results: An increase in lung damage was observed in Group 2 rats as compared to the control Group 1 (p<0.01), whereas much less lung damage was noted in the Group 3 rats as compared to the Group 2 rats (p<0.01). There was no statistically significant difference in the severity of histopathologically estimated lung damage between the rats of Group 3 and Group 4. Conclusion: We have shown through histopathological indices that high tidal volumes of ventilation cause VILI and that CA can prevent these.
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14
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2
