The Immunohistochemical Evaluation of the Expression of Intermediary Filaments, PCNA, P53 and MMP-9 in Feline Fibrosarcomas
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Dosyalar
Tarih
2023 Haziran
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Güncel çalışmada, kedilerin fibrosarkomlarında hücre proliferasyon indeksi, p53 tümor baskılayıcı gen durumu, tümor metastazı, invazyon kapasitesi ile hücresel orjinin ortaya konulması amacıyla Prolifere olan hücre nükleus antijeni (PCNA), Matriks Metalloproteinaz-9 (MMP-9), Vimentin, Alfa-Düz Kas Aktini (?-SMA), S-100, Desmin ve Pan- Sitokeratin (Pan-SK) gibi kanser markerları ve intermedier filamentlerin ekspresyonlarının immunohistokimyasal olarak değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada, Patoloji Anabilim Dalı’na rutin histopatolojik inceleme amacıyla getirilen 7 adet kediden alınan tümoral doku örnekleri kullanılmıştır. Histopatolojik değişikliklerin değerlendirilebilmesi amacıyla kesitlere Hematoksilen & Eozin boyaması uygulandı. İmmunohistokimyasal boyamalarda streptavidin-biotin peroksidaz kompleksi yöntemi kullanıldı. Bulgular: 7 spontane kutanöz fibrosarkom vakasının 5’i evre I, 1’i evre II ve 1’i ise evre III olarak saptandı. Tümöral dokulardaki belirgin kollajen bantlarının varlığı Masson Trikrom boyama ile ortaya konuldu. Vimentin ve ?-SMA boyanmaları yönünden tüm vakalar pozitifti. Pan SK, S-100 ve Desmin boyanmaları yönünden tümör hücreleri negatif reaksiyon verdi. Tüm vakalar PCNA yönünden pozitifti. Sadece 2 vaka p53 pozitif boyanma gösterdi. Bu 2 vaka, ileri evrelerdi. Sadece bir fibrosarkom vakasında (Evre III) tümör tipi dev hücrelerinde intrasitoplazmik MMP-9 ekspresyonları tespit edildi. Öneri: Sonuç olarak, çalışmada kullanılan tümör örneklem sayısı az olsa da, PCNA, p53 ve MMP-9’un kedilerin spontane fibrosarkomlarında hücre proliferasyonunun belirlenmesi, malignitenin derecelendirilmesi, tümör agresifliği ve davranışı ile prognozun saptanmasında oldukça kullanışlı markerlar olduğunu ortaya koymuştur.
Aim: The present study was aimed at the immunohistochemical evaluation of the expression of cancer indicators; including the Proliferating Cell Nucleus Antigen (PCNA), Matrix Metalloproteinase-9 (MMP-9), vimentin, alphasmooth muscle actin (?-SMA), S-100, desmin and pan-Cytokeratin (PanCK), as well as intermediary filaments, with a view to demonstrate the cell proliferation index, p53 tumour-suppressor gene status, tumour metastasis, invasion capacity and cellular origin of feline fibrosarcomas. Materials and Methods: The study material comprised of tumoral tissue samples from 7 cats, which were referred to the Pathology Department for routine histopathological examination. In order to evaluate the histopathological changes, the sections were stained with Haematoxylin and Eosin. Streptavidin-biotin peroxidase complex method was used for immunohistochemical staining. Results: Out of the seven spontaneous cutaneous fibrosarcoma cases, five were in stage I, one was in stage II, and one was in stage III. The presence of conspicuous collagen bands in the tumoral tissues was demonstrated by Masson’s trichrome (MT) staining. All cases stained positively for vimentin and ?-SMA. The tumoral cells reacted negatively for pan-CK, S-100 and desmin. All cases were immunopositive for PCNA. Two cases stained positively for p53. These two cases were advanced stage. Only a single case of fibrosarcoma (stage III) presented with intracytoplasmic MMP-9 expression in neoplastic giant cells. Conclusion: Despite only very few tumour samples having been examined in the present study, it was concluded that PCNA, p53 and MMP-9 served as highly useful indicators for determining cell proliferation, grading malignancy, detecting tumour aggressiveness and behaviour, as well as predicting prognosis in spontaneous feline fibrosarcomas.
Aim: The present study was aimed at the immunohistochemical evaluation of the expression of cancer indicators; including the Proliferating Cell Nucleus Antigen (PCNA), Matrix Metalloproteinase-9 (MMP-9), vimentin, alphasmooth muscle actin (?-SMA), S-100, desmin and pan-Cytokeratin (PanCK), as well as intermediary filaments, with a view to demonstrate the cell proliferation index, p53 tumour-suppressor gene status, tumour metastasis, invasion capacity and cellular origin of feline fibrosarcomas. Materials and Methods: The study material comprised of tumoral tissue samples from 7 cats, which were referred to the Pathology Department for routine histopathological examination. In order to evaluate the histopathological changes, the sections were stained with Haematoxylin and Eosin. Streptavidin-biotin peroxidase complex method was used for immunohistochemical staining. Results: Out of the seven spontaneous cutaneous fibrosarcoma cases, five were in stage I, one was in stage II, and one was in stage III. The presence of conspicuous collagen bands in the tumoral tissues was demonstrated by Masson’s trichrome (MT) staining. All cases stained positively for vimentin and ?-SMA. The tumoral cells reacted negatively for pan-CK, S-100 and desmin. All cases were immunopositive for PCNA. Two cases stained positively for p53. These two cases were advanced stage. Only a single case of fibrosarcoma (stage III) presented with intracytoplasmic MMP-9 expression in neoplastic giant cells. Conclusion: Despite only very few tumour samples having been examined in the present study, it was concluded that PCNA, p53 and MMP-9 served as highly useful indicators for determining cell proliferation, grading malignancy, detecting tumour aggressiveness and behaviour, as well as predicting prognosis in spontaneous feline fibrosarcomas.
Açıklama
Anahtar Kelimeler
Fibrosarkom, hücre proliferasyonu, intermedier filamentler, kedi, Cellular proliferation, feline, fibrosarcoma, intermediary filaments
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
39
Sayı
2
Künye
Karakurt, E., Aksoy, O., Beytut, E., Aydin, U., Dag, S., Yildiz, U., Yildiz, A., Kapcak, A. B., Koc, H., Alakan, A. M., (2023). The Immunohistochemical Evaluation of the Expression of Intermediary Filaments, PCNA, P53 and MMP-9 in Feline Fibrosarcomas. Eurasian Journal of Veterinary Sciences, 39(2), 84-91.
DOI: 10.15312/EurasianJVetSci.2023.402
