Alcoholic extract of Tarantula cubensis induces apoptosis in MCF-7 cell line.

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dc.contributor.authorEr, Ayse
dc.contributor.authorCorum, Orhan
dc.contributor.authorCorum, Duygu
dc.contributor.authorHitit, Mustafa
dc.contributor.authorDonmez, Huseyin
dc.contributor.authorGuzeloglu, Aydin
dc.date.accessioned2020-03-26T19:33:43Z
dc.date.available2020-03-26T19:33:43Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractTarantula cubensis Alcoholic Extract (TCAE) is a homeopathic agent used for treating many disorders. This study aimed to define the effects of TCAE on the breast carcinoma cell line (MCF-7). After various concentrations (10, 20, 40, 80 and 160 mu l/ml) of TCAE were applied to MCF-7 cells and the human embryonic kidney cell line (HEK293), the cells were incubated for 1, 3, 6, 9, 12, 24 and 48 h, followed by analysis by MTT assays. According to the results of the MTT assays, cells treated with 20 or 40 mu l/ml TCAE for 6 h were applied to apoptosis analysis by flow cytometry. Secreted levels of tumor necrosis factor alpha (TNF alpha), interleukin (IL)-1 beta, IL-6, IL-10, Interferon-gamma (IFN gamma), Transforming Growth Factor beta (TGF beta), and Nuclear Factor-kappa B (NF-kappa B) were measured using ELISAs. TNF alpha and TGF beta levels increased while IL-6 and IL-10 levels fluctuated in MCF-7 cells. In conclusion, our study suggests that TCAE may change the normal cancer physiology and lead to cell death by activating apoptosis in MCF-7 cells.en_US
dc.description.sponsorshipSUBAPKSelcuk University [14401119]en_US
dc.description.sponsorshipThis study is supported by SUBAPK (14401119). A part of the abstract was presented at the "5th International Congress of Molecular Medicine" 20-22 May 2015, Izmir, Turkey.en_US
dc.identifier.endpage3665en_US
dc.identifier.issn0970-938Xen_US
dc.identifier.issn0976-1683en_US
dc.identifier.issue8en_US
dc.identifier.startpage3660en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34772
dc.identifier.volume28en_US
dc.identifier.wosWOS:000403454800062en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherALLIED ACADen_US
dc.relation.ispartofBIOMEDICAL RESEARCH-INDIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectTarantula cubensis alcoholic extracten_US
dc.subjectTheranekronen_US
dc.subjectCytokinesen_US
dc.subjectMCF-7en_US
dc.subjectApoptosisen_US
dc.titleAlcoholic extract of Tarantula cubensis induces apoptosis in MCF-7 cell line.en_US
dc.typeArticleen_US

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