In Vitro Shoot Proliferation in Genus Endemic and Endangered Ornamental Plant Neotchihatchewia Isatidea (Boiss) Rauschert Using Different Organs
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Dosyalar
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SPRINGER
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The ornamental plant, Neotchihatchewia isatidea, is an endangered species of Turkey and threatened by complete extinction in the future. Therefore, in vitro multiplication of this species can be valuable for commercial production and germplasm conservation. Immature embryos of N. isatidea were cultured for initiation on Murashige and Skoog medium (MS) supplemented with N 6 -benzylamino-purine (BAP) and -naphthaleneacetic acid (NAA). Shoot primordia were visible within 5 - 6 weeks and the shoot primordia later developed into normal shoots 10 - 12 weeks after the culture initiation on calli developed from immature embryos. Shoot tips were also excised from developed plantlets for direct shoot organogenesis and cultured on MS shoot induction medium supplemented with BAP (0.5, 1.0 and 2.0 mg/l), kinetin (KIN) (0.5, 1.0 and 2.0 mg/l) and thidiazuron (TDZ) (0.05, 0.10 and 0.50 mg/l). Direct multiple shoots from shoot tips developed in most media tested. High shoot multiplication (3.73), high rooting (53 %) number of root per shoot (3.66) and survival ratio (46.6 %) were achieved.
Açıklama
Anahtar Kelimeler
Kaynak
In Vitro Cellular & Developmental Biology-animal
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
46
