Comparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochlorite

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dc.contributor.authorOk E.
dc.contributor.authorAdanir N.
dc.contributor.authorHakki S.
dc.date.accessioned2020-03-26T19:08:14Z
dc.date.available2020-03-26T19:08:14Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective: The purpose of this in vitro study was to compare the cytotoxicity of 0.5-4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts. Materials and Methods: About 0.5-4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fibroblasts. Viability of hPDL cells was evaluated with WST-1 (Cell Proliferation Reagent WST-1 Roche) test at 1, 24 and 72nd h. hPDL cells were plated at 20 × 103 cells per well in 96-well plates. Absorbance values were read in optical density 480 nm by ELISA plate reader spectrophotometer. The statistical differences between various groups were evaluated using one-way ANOVA, post-hoc Duncan's Multiple Range test using SAS software. Statistically, a significant difference was considered at P < 0.001. Results: According to the 1-h cytotoxicity results, 0.5% OES showed the least cytotoxic effect in test groups. There were not found any statistical significance between 1% OES and 2% CHX. About 5.25% NaOCl showed more cytotoxic effect than 1% OES and 2% CHX. In 24 and 72 h, different concentrations of OES, 5.25% NaOCl, 2% CHX solutions showed similar cytotoxic effect. Conclusions: Based on these results, 1% OES and 2% CHX showed similar results and less cytotoxic effect than 5.25% NaOCl. It could be considered as a favorable solution concentration when OES was used as root canal irrigation solution. © 2015 Dental Investigations Society.en_US
dc.identifier.doi10.4103/1305-7456.149630en_US
dc.identifier.endpage10en_US
dc.identifier.issn1305-7456en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage6en_US
dc.identifier.urihttps://dx.doi.org/10.4103/1305-7456.149630
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32804
dc.identifier.volume9en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherDental Investigations Societyen_US
dc.relation.ispartofEuropean Journal of Dentistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectChlorhexidine gluconateen_US
dc.subjectCytotoxicity testsen_US
dc.subjectOriganum extract solutionen_US
dc.subjectSodium hypochloriteen_US
dc.subjectWST-1 testen_US
dc.titleComparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochloriteen_US
dc.typeArticleen_US

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