IN VITRO ALTERATIONS IN RUMINAL PARAMETERS BY MEGASPHAERA ELSDENII INOCULATION ON SUBACUTE RUMINAL ACIDOSIS (SARA)

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dc.contributor.authorAlataş, Mustafa Selçuk
dc.contributor.authorUmucalilar, Huzur Derya
dc.date.accessioned2020-03-26T19:06:06Z
dc.date.available2020-03-26T19:06:06Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractSARA is a common and serious problem in well-managed and intensive dairy herds or beef cattle operations, because of triggering other metabolic disorders and causing lactation-fertility losses. It is a metabolic disease in dairy cattle that occurs during early and mid-lactation and has traditionally been characterized by low rumen pH, but lactic acid does not accumulate as in acute lactic acid acidosis. Managing the disease, rather than eliminating it, has been suggested in high-producing dairy herds. SARA was induced in vitro to appraise the effectiveness of Megasphaera elsdenii inoculation. Rumen fluid was collected from 2 ruminally cannulated Holstein heifers. Medium was prepared by mixing macromineral (200 ml), micromineral (0.1 ml), buffer (200 ml), reduction (40 ml) and resazurin (1 ml) solutions as well as distilled water (400 ml). The media was then added with a test diet consisting (g/kg) of 550-soluble starch, 260-glucose, 60-cellulose, 70-cellobiose and 60-tripticase, at levels of 10, 15, 20, 25, 30, 40, 50, 70 and 100 g/l. After determining its level causing SARA as reflected by pH (similar to 5.8) in preliminary experimentation, the substrate (test diet, 25 g/l) were incubated with presence of 0, 105, 106, and 107cfu M. elsdenii per ml at 39 degrees C for 24 h. Rumen parameters were analyzed by 2-way ANOVA. It is determined that most of the tested parameters are not influenced after inoculation of M. elsdenii, but the level of NH3-N (P< 0.002) and Butyric acid (P< 0.006) in rumen fluid, are observed to increase with the growth of bacterium level. When the bacterium is inoculated in the level of 7 cfu ml(-1), it is reached to the highest level of butyric acid (20.29 mM). It is showed that the existent evidence is similar to other studies. In conclusion, addition of M. elsdenii into media, one of the predominant lactate-utilizing bacteria failed to reverse SARA in vitro.en_US
dc.description.sponsorshipSelcuk UniversitySelcuk University [BAP 09102046]en_US
dc.description.sponsorshipWe would like to acknowledge the financial support of Selcuk University (PhD Thesis Project Number: BAP 09102046).en_US
dc.identifier.endpage79en_US
dc.identifier.issn2285-5750en_US
dc.identifier.issn2393-2260en_US
dc.identifier.pmid#YOKen_US
dc.identifier.startpage77en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32224
dc.identifier.volume58en_US
dc.identifier.wosWOS:000416371900010en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherUNIV AGRICULTURAL SCIENCES & VETERINARY MEDICINE BUCHARESTen_US
dc.relation.ispartofSCIENTIFIC PAPERS-SERIES D-ANIMAL SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectin vitroen_US
dc.subjectsubacute ruminal acidosis (SARA)en_US
dc.subjectMegasphaera elsdeniien_US
dc.subjectrumen fermentationen_US
dc.titleIN VITRO ALTERATIONS IN RUMINAL PARAMETERS BY MEGASPHAERA ELSDENII INOCULATION ON SUBACUTE RUMINAL ACIDOSIS (SARA)en_US
dc.typeArticleen_US

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