Assessment of the Protective Effects of Pomegranate Peel Extract and N-Acetyl Cysteine Alone or in Combination With Ornipural® Against Cadmium-Induced Bone Toxicity in Rat
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Dosyalar
Tarih
2024 Mart
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz Amaç: Bu çalışma, nar kabuğu ekstresi (PPE) ve N-asetil Sistein (NAC)'in tek başına veya ticari preparat Ornipural® (ORN) ile birlikte kullanımının, deneysel olarak kadmiyum (Cd) indüklenmiş toksisiteye karşı kemik metabolizması üzerindeki etkilerini belirlemek amacıyla yapılmıştır. Gereç ve Yöntem: Çalışmada her grupta 6 adet Wistar Albino sıçan olmak üzere toplam 36 hayvan kullanıldı. Hayvanlar kontrol, Cd, Cd+PPE, Cd+NAC, Cd+PPE+ORN ve Cd+NAC+ORN gruplarına atandı. Gruplardaki hayvanlara 8. haftanın sonunda kan örnekleri alındıktan sonra ötenazi uygulandı. Ötenazi sonrasında morfometrik ve histopatolojik incelemeler için kemikler maserasyona tabi tutuldu. Bulgular: Tedavi grupları ile Cd grubu arasında histopatolojik değişiklikler (osteoporotik değişiklikler ve kırmızı kemik iliğinde değişiklikler) ve biyobelirteçler (Ca, P ve Mg) açısından istatistiksel olarak anlamlı farklılıklar belirlendi (P<0.05). Öneri: Deneysel olarak indüklenmiş kadmiyum toksisitesine karşı uygulanan PPE, NAC ve ORN ile birlikte uygulanan tedavi kombinasyonlarının kemik metabolizması üzerinde olumlu etkilerinin olduğu belirlenmiş olmasına rağmen, uygulanan tedavi protokollerinin kesin etkililiğini belirlemek için tedavi süresini ve dozunu artırarak denemeler yapmanın faydalı olacağı düşünülmüştür.
Abstract Aim: The present study was conducted to determine the effects of pomegranate peel extract (PPE) and N-acetyl Cysteine (NAC) alone or in their treatment combinations with commercial preparation Ornipural® (ORN) on bone metabolism against experimentally cadmium (Cd)-induced toxicity in rats. Materials and Methods: Totally 36 animals were used in the study including 6 Wistar Albino rats in each group. The animals were assigned to control, Cd, Cd+PPE, Cd+NAC, Cd+PPE+ORN and Cd+NAC+ORN groups. The animals in the groups were euthanized after their blood samples were taken at the end of the 8th week. The bones were subjected to maceration for morphometric and histopathological examinations after euthanasia. Results: The statistically significant differences were determined between the treatment groups and Cd group in terms of histopathological changes (osteoporotic alterations and changes in red bone marrow) and biomarkers (Ca, P and Mg) (P<0.05). Conclusion: Although PPE, NAC and treatment combinations with ORN applied against experimentally induced cadmium toxicity were determined to have positive effects on bone metabolism, it has been thought that carrying out trials by increasing treatment duration and dose would be beneficial to determine definite efficacy of the applied treatment protocols.
Abstract Aim: The present study was conducted to determine the effects of pomegranate peel extract (PPE) and N-acetyl Cysteine (NAC) alone or in their treatment combinations with commercial preparation Ornipural® (ORN) on bone metabolism against experimentally cadmium (Cd)-induced toxicity in rats. Materials and Methods: Totally 36 animals were used in the study including 6 Wistar Albino rats in each group. The animals were assigned to control, Cd, Cd+PPE, Cd+NAC, Cd+PPE+ORN and Cd+NAC+ORN groups. The animals in the groups were euthanized after their blood samples were taken at the end of the 8th week. The bones were subjected to maceration for morphometric and histopathological examinations after euthanasia. Results: The statistically significant differences were determined between the treatment groups and Cd group in terms of histopathological changes (osteoporotic alterations and changes in red bone marrow) and biomarkers (Ca, P and Mg) (P<0.05). Conclusion: Although PPE, NAC and treatment combinations with ORN applied against experimentally induced cadmium toxicity were determined to have positive effects on bone metabolism, it has been thought that carrying out trials by increasing treatment duration and dose would be beneficial to determine definite efficacy of the applied treatment protocols.
Açıklama
Anahtar Kelimeler
Kemik toksisitesi, kadmiyum, N-asetil sistein, Ornipural®, nar kabuğu, Bone toxicity, cadmium, N-acetyl cysteine, Ornipural®, pomegranate peel
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
40
Sayı
1
Künye
Hatipoğlu, D., Demircioğlu, İ., Filikçi, K., Korkmaz, Y., Güngör, H., Demircioğlu M., Dik, B., (2024). Assessment of the Protective Effects of Pomegranate Peel Extract and N-Acetyl Cysteine Alone or in Combination With Ornipural® Against Cadmium-Induced Bone Toxicity in Rat. Eurasian Journal of Veterinary Sciences, (40)1, 24-33. DOI: 10.15312/EurasianJVetSci.2024.422
