Üç farklı adeziv sistemi ve klorheksidinin, dentinin fonksiyonel matriks metalloproteinaz aktivitelerine ve mikrotensile bağlanma dayanımına etkisi
Yükleniyor...
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmanın amacı; üç farklı adeziv sistemin (Adper Scotchbond Multi Purpose, Clearfil SE Bond, Adper Prompt L-Pop) ve bu üç farklı adeziv sistemiyle birlikte kullanılan klorheksidin (CHX)‟in, dentin matriksinde bulunan matriks metalloproteinaz enzimlerine olan etkilerini değerlendirmektir. Buna ilaveten bu çalışmada adeziv sistemlerin dentine bağlanma dayanımları in vitro koşullarda incelenmiştir. Bu çalışma jelatin zimografi yöntemi ve mikrotensile bağlanma dayanımı testi olmak üzere iki aşamadan oluşmaktadır. Jelatin zimografi yöntemi için 21 adet yeni çekilmiş, çürüksüz insan molar dişi kullanıldı. Dişlerin mineleri, kökleri ve pulpaları uzaklaştırıldı. Kuronal dentin örnekleri toz haline getirildi. Her biri 1 gr dentin tozu içeren 7 grup elde edildi. Bu gruplar; mineralize dentin grubu, Adper Scotchbond Multi Purpose uygulanan total etch grubu, Clearfil SE Bond uygulanan self etch grubu, Adper Prompt L-Pop uygulanan all in one grubu ve bu üç farklı
adeziv sistemin CHX ile birlikte uygulandığı gruplardır. Mikrotensile bağlanma dayanımı testi için 30 adet yeni çekilmiş, çürüksüz insan molar dişi kullanıldı. Dişlerin okluzalindeki minenin kaldırılmasıyla ortaya çıkan yüzeyel dentine, üç farklı adeziv sistemin ve CHX ile birlikte bu üç farklı adeziv sistemin uygulandığı 6 grup (n=5) elde edildi. Dişlere kompozit rezin uygulandıktan sonra distile suda, 37ºC etüvde 24 saat bekletildi. Dişlerden çubuk şeklinde kesitler alındıktan sonra, örnekler mikrotensile bağlanma dayanımı testine tabi tutuldu. Kopma yüzeylerinde meydana gelen başarısızlık tipleri stereomikroskop ile belirlendi ve kopma yüzeyleri taramalı elektron mikroskobu (SEM) kullanarak morfolojik olarak değerlendirildi. Verilerin istatistiksel analizi tek yönlü varyans analizi, t testi ve Scheffe yöntemi kullanılarak yapıldı (p=0,05). Jelatin zimografi yönteminin sonucunda, MMP 2 aktivitesi en yüksek total etch grubunda görüldü. Onu sırasıyla self etch grubu ve mineralize dentin grubu izledi. All in one grubu ve klorheksidin kullanılan gruplarda MMP aktivitesi görülmedi. Self etch ve all in one gruplarının bağlanma dayanımları arasında istatistiksel olarak anlamlı bir fark bulunmazken total etch grubunun bağlanma dayanımı diğerlerinden istatistiksel olarak anlamlı derecede yüksek bulundu. Klorheksidin adeziv sistemlerin bağlanma dayanımını arttırdı fakat istatistiksel olarak önemli derecede etkilemedi.
The purpose of this study was to evaluate the effects of three different adhesive systems (Adper Scotchbond Multi-Purpose, Clearfil SE Bond, Adper Prompt L-Pop) and three different adhesive systems in which subjected with chlorhexidine (CHX) to the matrix metalloproteinase enzymes in the dentin matrix. In addition to this, the bond strength of adhesive systems to dentin in vitro conditions was also investigated in this study. This study consisted of two parts: gelatin zymography and the microtensile bond strength test. Twenty one freshly extracted, non-carious human molar teeth were used for gelatin zymography. Enamel, roots and pulps of teeth were removed. Samples of coronal dentin were powdered. 7 groups each contains 1 gram dentin powder were obtained. These groups were, mineralized dentin group, total etch group which was subjected Adper Scotchbond Multi Purpose, self etch group which was subjected Clearfil SE Bond, all in one group which was subjected Adper Prompt L-Pop and other three groups which were subjected CHX for each group with these three different adhesive systems. Thirty freshly extracted, non-carious human molar teeth were used for microtensile bond strength test. 6 groups (n = 5) were obtained in which three different adhesive system and these three different adhesive system in conjunction with CHX were applied to the superfacial dentin resulting removal of the occlusal enamel. Teeth were stored in distilled water at 37 º C through for 24 h after the application of composite resin. Dentine sticks were obtained from these specimens and used for microtensile bond strength test. Types of failure on the surface of rupture were determined by stereomicroscope and fracture surfaces were evaluated morphologically by using scanning electron microscopy (SEM). Data was analyzed using with one-way analysis of variance, t-test and Scheffe method (p=0,05). As a result of the method of gelatin zymography showed that MMP 2 activity was the highest at the total etch group. It was followed by self etch group and the mineralized dentin respectively. MMP activity was not observed in all in one and subjected with chlorhexidine groups. Statistically a significant difference was not found between the bond strength of self etch and all in one group while total etch group was significantly higher than the others. CHX increased the bond strength of adhesive systems, but there was no significant statistically difference among these groups.
The purpose of this study was to evaluate the effects of three different adhesive systems (Adper Scotchbond Multi-Purpose, Clearfil SE Bond, Adper Prompt L-Pop) and three different adhesive systems in which subjected with chlorhexidine (CHX) to the matrix metalloproteinase enzymes in the dentin matrix. In addition to this, the bond strength of adhesive systems to dentin in vitro conditions was also investigated in this study. This study consisted of two parts: gelatin zymography and the microtensile bond strength test. Twenty one freshly extracted, non-carious human molar teeth were used for gelatin zymography. Enamel, roots and pulps of teeth were removed. Samples of coronal dentin were powdered. 7 groups each contains 1 gram dentin powder were obtained. These groups were, mineralized dentin group, total etch group which was subjected Adper Scotchbond Multi Purpose, self etch group which was subjected Clearfil SE Bond, all in one group which was subjected Adper Prompt L-Pop and other three groups which were subjected CHX for each group with these three different adhesive systems. Thirty freshly extracted, non-carious human molar teeth were used for microtensile bond strength test. 6 groups (n = 5) were obtained in which three different adhesive system and these three different adhesive system in conjunction with CHX were applied to the superfacial dentin resulting removal of the occlusal enamel. Teeth were stored in distilled water at 37 º C through for 24 h after the application of composite resin. Dentine sticks were obtained from these specimens and used for microtensile bond strength test. Types of failure on the surface of rupture were determined by stereomicroscope and fracture surfaces were evaluated morphologically by using scanning electron microscopy (SEM). Data was analyzed using with one-way analysis of variance, t-test and Scheffe method (p=0,05). As a result of the method of gelatin zymography showed that MMP 2 activity was the highest at the total etch group. It was followed by self etch group and the mineralized dentin respectively. MMP activity was not observed in all in one and subjected with chlorhexidine groups. Statistically a significant difference was not found between the bond strength of self etch and all in one group while total etch group was significantly higher than the others. CHX increased the bond strength of adhesive systems, but there was no significant statistically difference among these groups.
Açıklama
Anahtar Kelimeler
Adezyonlar, Adhesions, Dental bonding, Dentin, Klorheksidin, Chlorhexidine, Matriks metalloproteinazlar, Matrix metalloproteinases
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Kıran, A. Ö. (2012). Üç farklı adeziv sistemi ve klorheksidinin, dentinin fonksiyonel matriks metalloproteinaz aktivitelerine ve mikrotensile bağlanma dayanımına etkisi. Selçuk Üniversitesi, Yayımlanmış doktora tezi, Konya.
