Endotoksemide meloksikam uygulamasının serum vitamin düzeylerine etkisi
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Date
2012
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Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
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info:eu-repo/semantics/openAccess
Abstract
Araştırmanın amacı lipopolisakkarit uygulaması ile oluşturulan deneysel endotoksemide serum vitamin, sitokin ve organ hasar belirteçlerine meloksikamın etkisini belirlemek ve endotoksemi vakalarında meloksikamın kullanılabilirliliğini araştırmaktır. Araştırmada 50 adet erişkin erkek Sprague Dawley ırkı rat kullanıldı. Ratlardan 5 adedi kontrol (0 zaman) zaman verileri elde etmek için ayrıldıktan sonra, 45 adet rat 3 eşit gruba ayrılarak; 1. gruba lipopolisakkarit (4 mg, periton içi), 2. gruba meloksikam (50 mg/kg, periton içi) ve 3. gruba lipopolisakkarit (4 mg, periton içi) + meloksikam (50 mg/kg, periton içi) uygulamaları yapıldı. Uygulamalar sonrasında 2, 4 ve 8. saatlerde her gruptan 5 adet rattan anestezi altında kalpten kan alındı. Serumları çıkartıldıktan sonra retinol, ß-karoten, vitamin C, interlökin-1?, interlökin-1ß, interlökin-2 ve rutin biyokimyasal parametre düzeyleri belirlendi. Lipopolisakkarit uygulaması sonrasında düşen (p<0,05) ß-karoten düzeyi meloksikam tarafından engellenirken, yükselen (p<0,05) interlökin-1? düzeyinin artışı engellenemedi. Lipopolisakkarit uygulamasının kalp, karaciğer, böbrek hasar belirteçleri ile kolesterol ve trigliserit düzeylerinde artışlara (p<0,05) neden olduğu, meloksikam uygulamasının kreatin kinaz-MB ve kolesterol düzeylerinde oluşan yükselmeleri engellediği ancak üre ve trigliserit düzeylerini daha fazla yükselttiği belirlendi. Sonuç olarak endotoksemilerde oluşan vitamin kayıpları ile kalp hasarını engellemede meloksikamın faydalı olabileceği ve endotoksemilerin akut döneminde nonsteroid antiinflammatuar ilaç ile vitamin takviyesinin faydalı olabileceği kanaatine varıldı.
The aim of this study is to evaluate the effects of meloxicam on serum vitamin, cytokine and organ damage indicator levels during lipopolysaccharide-induced endotoxemia and investigate administration of meloxicam on endotoxemia issues. Fifty male adult Sprague Dawley rats were used in this research. Five rats were reserved to obtain control (time 0) time data, then 45 rats were divided into 3 equal groups; 1. group received lipopolysaccharide (4 mg, intraperitoneal), 2. group received meloxicam (50 mg/kg, intraperitoneal) and 3. group received lipopolysaccharide (4 mg, intraperitoneal) plus meloxicam (50 mg/kg, intraperitoneal). Blood samples were collected from heart of 5 rats in each groups under anesthesia at 2, 4 and 8 hours after administrations. Serum retinol, ß-carotene, vitamin C, interleukin-1?, interleukin-1ß, interleukin-2 and routine biochemical values were measured. After lipopolysaccharide administration, decreased ß-carotene level (p<0,05) was inhibited by meloxicam while increased interleukin-1? level (p<0,05) was not prevented. Lipopolysaccharide caused increase in damage indicator levels of heart, liver, kidney besides cholesterol and triglyceride (p<0,05) while meloxicam administration inhibited increase in creatinine kinase-MB and cholesterol levels but caused more increase in urea and triglyceride levels. As a result, meloxicam may be useful in inhibiting vitamin loses and heart damages caused by endotoxemia, and non-steroidal anti-inflammatory drug and vitamin supplementation may be useful during acute phase of endotoxemia.
The aim of this study is to evaluate the effects of meloxicam on serum vitamin, cytokine and organ damage indicator levels during lipopolysaccharide-induced endotoxemia and investigate administration of meloxicam on endotoxemia issues. Fifty male adult Sprague Dawley rats were used in this research. Five rats were reserved to obtain control (time 0) time data, then 45 rats were divided into 3 equal groups; 1. group received lipopolysaccharide (4 mg, intraperitoneal), 2. group received meloxicam (50 mg/kg, intraperitoneal) and 3. group received lipopolysaccharide (4 mg, intraperitoneal) plus meloxicam (50 mg/kg, intraperitoneal). Blood samples were collected from heart of 5 rats in each groups under anesthesia at 2, 4 and 8 hours after administrations. Serum retinol, ß-carotene, vitamin C, interleukin-1?, interleukin-1ß, interleukin-2 and routine biochemical values were measured. After lipopolysaccharide administration, decreased ß-carotene level (p<0,05) was inhibited by meloxicam while increased interleukin-1? level (p<0,05) was not prevented. Lipopolysaccharide caused increase in damage indicator levels of heart, liver, kidney besides cholesterol and triglyceride (p<0,05) while meloxicam administration inhibited increase in creatinine kinase-MB and cholesterol levels but caused more increase in urea and triglyceride levels. As a result, meloxicam may be useful in inhibiting vitamin loses and heart damages caused by endotoxemia, and non-steroidal anti-inflammatory drug and vitamin supplementation may be useful during acute phase of endotoxemia.
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Biyokimyasal değerler, Endotoksemi, Meloksikam, Sitokinler, Vitaminler, Biochemical values, Cytokines, Endotoxemia, Meloxicam, Vitamins
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Yurt, A. Ö. (2012). Endotoksemide meloksikam uygulamasının serum vitamin düzeylerine etkisi. Selçuk Üniversitesi, Yayımlanmış yüksek lisans tezi, Konya.
