Evaluation of Nigella sativa's cold press oil as vaccine adjuvant
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veteriner Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Organik ve bitkisel kökenli, düşük düzeyde toksik olan, daha ucuz ve etkili bir adjuvant geliştirilmesi amacıyla soğuk bası Nigella sativa yağı(SBNSY) ve bu yağın temel bileşenlerinden timokiononun adjuvant potansiyeli incelendi. Gereç ve Yöntem: SBNSY veya timokionon ile ovalbumin verilerek oluşturulan spesifik antikorların ölçümü değerlendirildi. Swiss albino fareler (n=36) A0 (kontrol), A1 (timokionon), A2 (SBNSY), A3 (içeriği; timokionon miktarında % 10 oranında arttırım sureti ile değiştirilmiş olan SBNSY) ve A4 (ticari adjuvant, Al(OH)3) olmak üzere farklı şekillerde verildi. Etkinin değerlendirilmesi, immünizasyondan sonra ovalbumine karşı oluşan spesifik antikoların I-ELISA ile ölçümü yolu ile ve laboratuvarımızda yapıldı. Bulgular: Serum ve konjugatın optimal sulandırmaları sırası ile 1/200 ve 1/40.000 olarak bulundu. 450 nm’de okunan ortalama OD değerleri kontrol (A0), A1, A2, A3 ve A4 grupları için sırası ile 0,043 (± 0,002), 0,668 (± 0,074), 0,644 (± 0,018), 0,675 (± 0,066), ve 0,745 (± 0,09) idi. Öneri: Her üç formulasyon da (A1, A2, A3), kontrol grubuna göre ovalbumine karşı sıvısal immün yanıtın oluşturulmasında ticari adjuvant (A4) formulasyonu kadar etkili bulundu (p > 0,05). Farede, bu çalışmada denenen her bir Nigella sativa temelli adjuvant adayının potansiyel olarak Al(OH)3’e alternatif olabileceği kanaatine varıldı.
Aim: In order to identify an organic and plant based, less toxic, cheaper and more effective adjuvant, a cold pressed oil of Nigella sativa (CPNSO) and one of the essential components of Nigella sativa oil (NSO), thymoquinone (Thymoquinone ) were investigated as adjuvant candidates. Materials and Methods: Adjuvant potentials of the both were measured by examining specific antiibody titers to ovalbumin given in presence of either of CPNSO or thymoquinone, in vivo. Such potentials of thymoquinone alone (A1), CPNSO (A2), mCPNSO (experimentally formed by addition of thymoquinone to CPNSO making up mCPNSO that was contained 10% more thymoquinone than original CPNSO did; A3) and Al(OH)3 (A4) in presence of ovalbumin in Swiss albino mice (n=36, female). The effects were determined by measuring anti-ovalbumin antibodies after immunization to ovalbumin by home-made ELISA in mice. Results: Sera and conjugate were optimally diluted 1/200 and 1/40.000, respectively. Mean OD values at 450 nm of the groups control (A0), A1, A2, A3 and A4 were 0,043 (± 0,002), 0,668 (± 0,074), 0,644 (± 0,018), 0,675 (± 0,066), and 0,745 (± 0,09), respectively. Conclusion: By comparison control, all three (A1, A2, A3) of the test formulations were found to be as effective as commercial (A4) formulation in triggering humoral response to ovalbumin (p > 0,05). Therefore, each of Nigella sativa based adjuvant candidates has an alternative potential to Al(OH)3 in the mouse.
Aim: In order to identify an organic and plant based, less toxic, cheaper and more effective adjuvant, a cold pressed oil of Nigella sativa (CPNSO) and one of the essential components of Nigella sativa oil (NSO), thymoquinone (Thymoquinone ) were investigated as adjuvant candidates. Materials and Methods: Adjuvant potentials of the both were measured by examining specific antiibody titers to ovalbumin given in presence of either of CPNSO or thymoquinone, in vivo. Such potentials of thymoquinone alone (A1), CPNSO (A2), mCPNSO (experimentally formed by addition of thymoquinone to CPNSO making up mCPNSO that was contained 10% more thymoquinone than original CPNSO did; A3) and Al(OH)3 (A4) in presence of ovalbumin in Swiss albino mice (n=36, female). The effects were determined by measuring anti-ovalbumin antibodies after immunization to ovalbumin by home-made ELISA in mice. Results: Sera and conjugate were optimally diluted 1/200 and 1/40.000, respectively. Mean OD values at 450 nm of the groups control (A0), A1, A2, A3 and A4 were 0,043 (± 0,002), 0,668 (± 0,074), 0,644 (± 0,018), 0,675 (± 0,066), and 0,745 (± 0,09), respectively. Conclusion: By comparison control, all three (A1, A2, A3) of the test formulations were found to be as effective as commercial (A4) formulation in triggering humoral response to ovalbumin (p > 0,05). Therefore, each of Nigella sativa based adjuvant candidates has an alternative potential to Al(OH)3 in the mouse.
Açıklama
Anahtar Kelimeler
Timokionon, Adjuvant, Ovalbümin, Fare, Thymoquinone, Adjuvant, Ovalbumin, Mouse
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
3
Künye
Uçan, U. S., Sakmanoğlu, A., Uslu, A., Sayın, Z., Oğlakçı Cansoy, E., (2020).Evaluation of Nigella sativa's cold press oil as vaccine adjuvant. Eurasian Journal of Veterinary Sciences, 36 (3), 199-203. DOI: 10.15312/EurasianJVetSci.2020.279
