Potency of Rhodococcus equi culture filtrate supernatant proteins antigen for skin test in the diagnosis of Rhodococcus equi in foals
Yükleniyor...
Dosyalar
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Araştırma Rhodococcus equi protein antijenlerinin deri testinin geliştirilmesi için dizayn edildi. Gereç ve Yöntem: R. equi ile enfekte 10 adet Arap ati tayına 0.1 mL (12.5 µg/mL) Rhodococcus untiji ve 10 adet sağlıklı kontrol grubuna 0.1 mL PBS bacak bölgesine deri içi yolla enjekte edildi. Deri kalınlıkları uygulama öncesi ve sonrası 24, 48 ve 72 saatlerde ölçüldü. Deri biyopsisi uygulama sonrasında 72. saatte yapıldı. Bulgular: Deri reaksiyonları uygulama sonrası 24. saatte hafif, 72. saatte ise yoğun gözlendi. Yapılan biyopsilerde makrofaj, nötrofil ve eozinofil gibi yangi hücreleri belirlendi. Kontrol grubu taylarda uygulama sonrası gözle görülen bir reaksiyon gözlenmedi. Öneri: Rhodococcus-kültür antijeninin, R. equi'ye maruz kalan veya enfekte olan taylarda hastalığın tanısında diagnostik öneminin olabileceği belirlendi. Mevcut araştırmanın sonuçları dikkate alındığında, R equi ile enfekte haralarda deri testinin hastalığın tanısında umut vaat etmektedir.
Alm: This study was designed to developing skin test proteins antigen prepared from Rhodococcus equi isolates. Materials and Methods: Ten Arabian foals experimentally Infected with R. equi were injected intradermaly with 0.1 mL (12.5 µg/mL) Rhodococcus antigen and 0.1 mL PBS as control each treatment at one side of neck region, while 10 healthy foals had the same treatment and used as control group. Skin thickness was measured prior and at 24, 48 and 72 hours post injection by skin caliber: Skin biopsy was taken om the site of reaction at 72 hours post injection. Results: All infected foals had slightly skin reaction at 24 hours, and maximum skin reaction was determined at 72 hours post inoculation. Inflammatory cells including macrophages, neutrophils and eosinophils were present in the sections prepared from blopsy obtained from positive skin reaction. All control foals revealed no visible reaction in the site of injection. Conclusion: Partially purified Rhodococcus-culture antigen is potential diagnostic antigens able to detect foals previously infected or exposed to R. equi infection. Our data provide a promising basis for the future development of screening diagnostic skin tests for R. equi infection in infected farms.
Alm: This study was designed to developing skin test proteins antigen prepared from Rhodococcus equi isolates. Materials and Methods: Ten Arabian foals experimentally Infected with R. equi were injected intradermaly with 0.1 mL (12.5 µg/mL) Rhodococcus antigen and 0.1 mL PBS as control each treatment at one side of neck region, while 10 healthy foals had the same treatment and used as control group. Skin thickness was measured prior and at 24, 48 and 72 hours post injection by skin caliber: Skin biopsy was taken om the site of reaction at 72 hours post injection. Results: All infected foals had slightly skin reaction at 24 hours, and maximum skin reaction was determined at 72 hours post inoculation. Inflammatory cells including macrophages, neutrophils and eosinophils were present in the sections prepared from blopsy obtained from positive skin reaction. All control foals revealed no visible reaction in the site of injection. Conclusion: Partially purified Rhodococcus-culture antigen is potential diagnostic antigens able to detect foals previously infected or exposed to R. equi infection. Our data provide a promising basis for the future development of screening diagnostic skin tests for R. equi infection in infected farms.
Açıklama
Anahtar Kelimeler
Rhodococcus equi, tay, deri testi, foal, skin test
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
27
Sayı
3
Künye
Al-Salihi, K., (2011). Potency of Rhodococcus Equi Culture Filtrate Supernatant Proteins Antigen for Skin Test in The Diagnosis of Rhodococcus Equi in Foals. Eurasian Journal of Veterinary Sciences, 27 (3), 161-165.
