Behçet hastalarında asimetrik dimetilarginin düzeyleri
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Behçet Hastalığı (BH), etiyolojisi tam olarak anlaşılmamış, kronik, inflamatuvar multisistemik bir vaskülittir. Nitrik Oksit (NO), endotel hücrelerinde Nitrik Oksit Sentaz (NOS) enzimi tarafından sentezlenen, vasküler sistem için önemi olan bir moleküldür. Asimetrik dimetilarjinin (ADMA) NOS'ın endojen inhibitörüdür. Bu çalışmada BH'larında ADMA düzeyleri ve ataklarla ilgisinin araştırılması amaçlandı.Metod: 34 Behçet hastası ve 34 sağlıklı kontrol çalışmaya dâhil edildi. Serum ADMA, arjinin ve sitrülin düzeyleri HPLC yöntemi ile saptandı. ESR ve CRP düzeyleri rutin yöntemlerle analiz edildi.Bulgular: Literatür bilgilerine ters olarak Behçet hastalarında ADMA düzeyleri kontrol grubuna göre düşük bulundu. Bunun aksine Arjinin ve Sitrülin düzeyleri Behçet hastalarında anlamlı derecede yüksek bulundu. Hasta grubunda Arjinin/ADMA oranları yüksek bulundu. ADMA ve CRP düzeyleri arasında ters bir korelasyon vardı. Arjinin değerleri de sitrülin ve CRP düzeyleri ile koreleydi. Sonuç: BH da genç yaşta atakların sayısının fazla olmasının sebeblerinden birisi düşük ADMA düzeyleri olabilir. Bizim çalışmamız hasta grubundaki düşük ADMA düzeylerine, yaşlanma ile BH alevlenmelerini ve NO aracılıklı inflamasyonu azaltması nedeniyle muhtemel önleyici bir rol yüklemektedir
Objective: Behçet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients.Methods: The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods.Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels.Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging
Objective: Behçet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients.Methods: The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods.Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels.Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging
Açıklama
Anahtar Kelimeler
Biyokimya ve Moleküler Biyoloji
Kaynak
Türk Biyokimya Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
39
Sayı
3
