Günlük kullanılan spor tipi ayakkabılarda fungal kontaminasyonun belirlenmesi
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi, Mantarcılık Uygulama ve Araştırma Merkezi Müdürlüğü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, günlük kullanılan spor tipi ayakkabıların iç kısımlarında fungal kontaminasyonun belirlenmesi amaçlanmıştır. 50 çift spor tipi ayakkabının iç kısımlarından steril eküvyonla sürüntü yapılarak alınan örnekler, Rosebengal Chloramphenicol Agar içeren petri kaplarına inoküle edilmiş ve 28oC’de iki hafta inkübasyona bırakılmıştır. Gelişen fungus kolonileri sayılarak Czapek Dox Agar ve Malt Extract Agar içeren petrilere teşhis amacı ile pasajlanmış ve 7-14 gün inkübe edilmiştir. İnkübasyondan sonra, elde edilen funguslar ilgili referanslar dikkate alınarak yapılan makroskobik ve mikroskobik incelemeler sonucunda teşhis edilmişlerdir. Acremonium, Alternaria, Aspergillus, Cladosporium, Fusarium, Penicillium, Phoma ve Trichophyton cinslerine ait 15 fungus türü izole edilmiştir. Elde edilen funguslar içerisinde tür zenginliği bakımından Penicillium, koloni sayısı bakımından ise Cladosporium cinslerinin dominant olduğu belirlenmiştir. 35 çift ayakkabı fungal kontaminasyon bakımından temiz bulunmuştur. Ayakkabılardan izole edilen funguslar dünya çapında dağılım gösteren yaygın funguslardır. Cladosporium dışındakiler, insanlarda çeşitli mikozlara ve mikotoksikozlara sebep olabilmektedirler. Ayakkabılardan ayaklara ve vücudun diğer kısımlarına bulaşabilirler ve enfeksiyonlara neden olabilirler. Böylece ayak ve vücut sağlığını etkileyebilirler. Bu bakımdan doğru ayakkabı seçimi yapılmalı ve bireysel hijyene önem verilmelidir.
In this study, it was intended to determine of fungal contamination in casual sports type shoes. The samples were taken by using moistened swap sticks from the inside of the 50 pairs of sports type shoes. Samples were inoculated to petri dishes containing Rosebengal Chloramphenicol Agar and they were incubated at 280C for two weeks. The growing fungi colonies were counted and were taken to petri dishes containing Czapek Dox Agar and Malt Extract Agar for identification and they were incubated for 7-14 days. After incubation, obtained fungi were diagnosed for macroscopic and microscopic features by analyzing the relevant references. 15 fungal species belonging to genera of Acremonium, Alternaria, Aspergillus, Cladosporium, Fusarium, Penicillium, Phoma and Trichophyton were obtained. Penicillium was found dominantly in terms of the number of species and Cladosporium was found in high frequency in terms of the number of colony. 35 pairs of casual sports type shoes were found clean regarding to fungal contamination. The isolated fungi from shoes are world-wide spread fungi. Except the Cladosporium the others cause various mycosis and mycotoxicosis in humans. They can transmit from shoes to feet and to other parts of the body and they can cause infections which can effect the health of feet and body. Therefore, suitable shoes should be chosen carefully and individual hygiene should be regarded.
In this study, it was intended to determine of fungal contamination in casual sports type shoes. The samples were taken by using moistened swap sticks from the inside of the 50 pairs of sports type shoes. Samples were inoculated to petri dishes containing Rosebengal Chloramphenicol Agar and they were incubated at 280C for two weeks. The growing fungi colonies were counted and were taken to petri dishes containing Czapek Dox Agar and Malt Extract Agar for identification and they were incubated for 7-14 days. After incubation, obtained fungi were diagnosed for macroscopic and microscopic features by analyzing the relevant references. 15 fungal species belonging to genera of Acremonium, Alternaria, Aspergillus, Cladosporium, Fusarium, Penicillium, Phoma and Trichophyton were obtained. Penicillium was found dominantly in terms of the number of species and Cladosporium was found in high frequency in terms of the number of colony. 35 pairs of casual sports type shoes were found clean regarding to fungal contamination. The isolated fungi from shoes are world-wide spread fungi. Except the Cladosporium the others cause various mycosis and mycotoxicosis in humans. They can transmit from shoes to feet and to other parts of the body and they can cause infections which can effect the health of feet and body. Therefore, suitable shoes should be chosen carefully and individual hygiene should be regarded.
Açıklama
DOI: 10.30708/mantar.416832
Anahtar Kelimeler
Fungal kontaminasyon, Ayakkabı kontaminantları, Fungus, Bireysel hijyen, Fungal contamination, Contaminants of shoes, Individual hygiene
Kaynak
Mantar Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
9
Sayı
Künye
Özkan, V. K., Uzun, M. T., Gündoğan, M. T. (2018). Günlük kullanılan spor tipi ayakkabılarda fungal kontaminasyonun belirlenmesi. Mantar Dergisi, 9, (2), 182-187.
