Türkiye'de yetiştirilen bazı keçi ırklarında büyüme hormonu geni (GH-1), insülin benzeri büyüme faktörleri (IGF-1) ve pitüiter spesifik transkripsiyon faktörü-1 (PİT-1) genleri polimorfizmleri
Yükleniyor...
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu araştırmada büyümeye ve gelişmeye etkili olan ve kas gelişmesinin düzenlenmesinde memelilerde aday gen olarak kabul edilen Hipofiz Spesifik Transkripsiyon Faktörü-1 (Pituitary-specific Transcription Factor; Pit-1) geninin ekzon 6, (Insulin Like Growth Factor-1; IGF-1) geninin ekzon 4 ve intron 4 ve (Growth Hormone-1; GH-1) geninin ekzon 2 ve 3 bölgelerinin polimorfizm bakımından incelenmesi amaçlanmıştır. Elli iki baş Kıl, 51 baş Ankara keçisi, 50 baş Kilis, 50 baş Honamlı, 50 baş Halep ve 50 baş Saanen keçisi olmak üzere 6 farklı ırktan oluşan toplam 303 baş keçide GH-1 geni HaeIII, IGF-1 HaeIII ve Pit-1 PstI restriksiyon enzimleri ile kesilmiştir. PCR-RFLP (Polimeraz Zincir Reaksiyonu ve Restriksiyon Parça Uzunluk Polimorfizmi) kullanılarak Pit-1 geni PstI polimorfizmi (450 bç), IGF-1 geni HaeIII polimorfizmi (363 bç) ve GH-1 geni HaeIII polimorfizmi (422 bç) ile genotiplendirilmiştir. Pit-1 geni PstI polimorfizmi bakımından T ve C allel frekansları Kıl, Ankara, Honamlı, Halep, Sannen ve Kilis ırklarında sırasıyla 0.9038 ve 0.0962, 0.902 ve 0.098, 0.8900 ve 0.1100, 0.9300 ve 0.0700, 0.8300 ve 0.1700 ile 0.9100 ve 0.0900 olarak tespit edilmiştir. Bütün ırklarda CC genotipi tespit edilmemiş olup, TT ve TC genotip frekansları ise sırasıyla Kıl keçilerinde 0.808 ve 0.192 (P>0.05); Ankara keçilerinde 0.804 ve 0.196 (P>0.05); Honamlı keçilerinde 0.780 ve 0.220 (P>0.05); Halep keçilerinde 0.860 ve 0.140 (P>0.05); Saanen keçilerinde 0.660 ve 0.340 (P>0.05)ve Kilis keçilerinde 0.820 ve 0.180 (P>0.05) olarak bulunmuştur. Genel ırklar dikkate alındığında T ve C allel frekanslarını 0.8944 ve 0.1056, TT, TC ve CC genotip frekanslarını ise 0.789, 0.211 ve 0.000 olarak tespit edilmiştir (P<0.05). Pit-1 geni ekzon 6 bölgesi PstI polimorfizmi bakımından ırklar bazında genel değerlendirme yapıldığında populasyonun Hardy-Weinberg dengesinde olmadığı belirlenmiştir (P<0.05). IGF-1 geni HaeIII enzim kesimi sonucu iki allel (A ve B) ve üç genotip (AA, BB ve AB) belirlenmiştir. Kıl, Ankara, Honamlı, Halep, Saanen ve Kilis keçi ırkları için A ve B allel frekansları sırasıyla 0.3558 ve 0.6442, 0.5784 ve 0.4216, 0.2100 ve 0.7900, 0.7300 ve 0.2700, 0.7800 ve 0.2200, 0.2900 ve 0.7100 olarak bulunmuştur. AA, AB ve BB genotip frekansları bakımından ise sırasıyla 0.250, 0.212 ve 0.538 (P<0.05); 0.451, 0.255 ve 0.294 (P<0.05); 0.060, 0.300 ve 0.640 (P>0.05); 0.600, 0.260 ve 0.140 (P<0.05); 0.700, 0.160 ve 0.140 (P<0.05); 0.200, 0.180 ve 0.620 (P<0.05) olarak bulunmuştur.. Bütün ırklar dikkate alındığında A ve B allel frekansları 0.4901 ve 0.5099, AA, AB ve BB genotip frekansları ise 0.376, 0.228 ve 0.396 (P<0.05) olarak tespit edilmiştir. Çalışılan ırklarda populasyonun Hardy-Weinberg dengesinde olmadığı belirlenmiştir (P<0.05). GH-1 geninin HaeIII enzimi ile kesimi sonucu iki allel (A ve B) ve üç genotip (AA, BB ve AB) belirlenmiştir. Kıl, Ankara, Honamlı, Halep, Saanen ve Kilis keçi ırkları için A ve B allel frekansları sırasıyla 0.4038 ve 0.5962, 0.4314 ve 0.5686, 0.4600 ve 0.5400, 0.4500 ve 0.5500, 0.3800 ve 0.6200, 0.5400 ve 0.4600 olarak bulunmuştur. AA, AB ve BB genotip frekansları bakımından ise sırasıyla 0.019, 0.769 ve 0.212 (P<0.05); 0.235, 0.392 ve 0.372 (P>0.05); 0.080, 0.760 ve 0.160 (P<0.05); 0.060, 0.780 ve 0.160 (P<0.05); 0.160, 0.440 ve 0.400 (P>0.05) ve 0.160, 0.760 ve 0.080 (P<0.05) olarak bulunmuştur. Bütün ırklar dikkate alındığında A ve B allel frekansları 0.4439 ve 0.5561, AA, AB ve BB genotip frekansları ise 0.119, 0.650 ve 0.231 (P<0.05) olarak tespit edilmiştir. Genel populasyonun Hardy-Weinberg dengesinde olmadığı belirlenmiştir (P<0.05).
The purpose of this study is to determine Pituitary-specific Transcription Factor-1 (Pit-1) gene exon 6, insulin-like growth factors (IGF-1) gene exon 4 and intron 4 and growth hormone (GH-1) gene exon 2 and 3 regions in terms of polymorphism. Animal materials of the study consist of a total of 303 goats including 52 Hair, 51 Angora, 50 Kilis, 50 Honamlı, 50 Aleppo and 50 heads of Saanen breeds. GH-1 and IGF-1genes were cut with HaeIII and Pit-1 gene were cut with PstI restriction enzymes. Pit-1 gene PstI polymorphism (450 bp), IGF-1 gene HaeIII polymorphism (363 bp) and GH-1 gene HaeIII polymorphism (422 bp) were genotyped by using PCR-RFLP (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism) Pit-1 gene in terms of PstI polymorphism, T and C allele frequencies were determined as 0.9038 and 0.0962; 0.902 and 0.098; 0.8900 and 0.1100; 0.9300 and 0.0700; 0.8300 and 0.1700 and 0.9100 and 0.0900 for Hair, Angora, Kilis, Honamlı, Aleppo and Saanen breeds respectively. CC genotype was not detected in all breeds. TT and TC genotype frequencies were found to be 0.808 and 0.192 in Hair goats (P>0.05), 0.804 and 0.196 (P>0.05) in Ankara goats; 0.780 and 0.220 (P>0.05) in Honamlı goats; 0.860 and 0.140 (P>0.05) in Aleppo goats; respectively 0.660 and 0.340 (P>0.05) in Saanen goats and 0.820 and 0.180 (P>0.05) in Kilis goats respectively. When the general breeds were taken into consideration, T and C allele frequencies were found to be 0.8944 and 0.1056, and TT, TC and CC genotype frequencies were 0.789, 0.211 and 0.000 (P<0.05). In terms of PstI polymorphism in the exon 6 region of the Pit-1 gene, it was determined that the population was not in Hardy-Weinberg equilibrium (P<0.05). IGF-1 gene HaeIII restriction enzymes results were identified two alleles (A and B) and three genotypes (AA, BB and AB). A and B allele frequencies for Hair, Angora, Honamlı, Aleppo, Saanen and Kilis breeds were found to be 0.3558 and 0.6442, 0.5784 and 0.4216, 0.2100 and 0.7900, 0.7300 and 0.2700, 0.7800 and 0.2200, 0.2900 and 0.7100, respectively. AA, AB and BB genotype frequencies were found to be 0.250, 0.212 and 0.538 (P<0.05), 0.451, 0.255 and 0.294 (P<0.05), 0.060, 0.300 and 0.640 (P>0.05), 0.600, 0.260 and 0.140 (P<0.05), 0.700, 0.160 and 0.140 (P<0.05), 0.200, 0.180 and 0.620 (P<0.05) respectively. When all breeds were taken into consideration, A and B allel frequencies were found to be 0.4901 and 0.5099, and AA, AB and BB genotype frequencies were found to be 0.376, 0.228 and 0.396 (P<0.05). It was determined that population was in not Hardy-Weinberg equilibrium (P<0.05). GH-1 gene HaeIII restriction enzymes results were identified two alleles (A and B) and three genotypes (AA, BB and AB). A and B allel frequencies for Hair, Angora, Honamlı, Aleppo, Saanen and Kilis breeds were found to be 0.4038 and 0.5962, 0.4314 and 0.5686, 0.4600 and 0.5400, 0.4500 and 0.5500, 0.3800 and 0.6200, 0.5400 and 0.4600, respectively. AA, AB and BB genotype frequencies were found to be 00.019, 0.769 and 0.212 (P<0.05), 0.235, 0.392 and 0.32 (P>0.05), 0.080, 0.760 and 0.160 (P<0.05), 0.060, 0.780 and 0.160 (P<0.05), 0.160, 0.440 and 0.400 (P>0.05), 0.160, 0.760 and 0.080 (P<0.05), respectively. When all breeds were taken into consideration, A and B allel frequencies were found to be 0.4439 and 0.5561, and AA, AB and BB genotype frequencies were found to be 0.119, 0.650 and 0.231 (P<0.05). It was determined that the population studied was not in Hardy-Weinberg equilibrium (P<0.05).
The purpose of this study is to determine Pituitary-specific Transcription Factor-1 (Pit-1) gene exon 6, insulin-like growth factors (IGF-1) gene exon 4 and intron 4 and growth hormone (GH-1) gene exon 2 and 3 regions in terms of polymorphism. Animal materials of the study consist of a total of 303 goats including 52 Hair, 51 Angora, 50 Kilis, 50 Honamlı, 50 Aleppo and 50 heads of Saanen breeds. GH-1 and IGF-1genes were cut with HaeIII and Pit-1 gene were cut with PstI restriction enzymes. Pit-1 gene PstI polymorphism (450 bp), IGF-1 gene HaeIII polymorphism (363 bp) and GH-1 gene HaeIII polymorphism (422 bp) were genotyped by using PCR-RFLP (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism) Pit-1 gene in terms of PstI polymorphism, T and C allele frequencies were determined as 0.9038 and 0.0962; 0.902 and 0.098; 0.8900 and 0.1100; 0.9300 and 0.0700; 0.8300 and 0.1700 and 0.9100 and 0.0900 for Hair, Angora, Kilis, Honamlı, Aleppo and Saanen breeds respectively. CC genotype was not detected in all breeds. TT and TC genotype frequencies were found to be 0.808 and 0.192 in Hair goats (P>0.05), 0.804 and 0.196 (P>0.05) in Ankara goats; 0.780 and 0.220 (P>0.05) in Honamlı goats; 0.860 and 0.140 (P>0.05) in Aleppo goats; respectively 0.660 and 0.340 (P>0.05) in Saanen goats and 0.820 and 0.180 (P>0.05) in Kilis goats respectively. When the general breeds were taken into consideration, T and C allele frequencies were found to be 0.8944 and 0.1056, and TT, TC and CC genotype frequencies were 0.789, 0.211 and 0.000 (P<0.05). In terms of PstI polymorphism in the exon 6 region of the Pit-1 gene, it was determined that the population was not in Hardy-Weinberg equilibrium (P<0.05). IGF-1 gene HaeIII restriction enzymes results were identified two alleles (A and B) and three genotypes (AA, BB and AB). A and B allele frequencies for Hair, Angora, Honamlı, Aleppo, Saanen and Kilis breeds were found to be 0.3558 and 0.6442, 0.5784 and 0.4216, 0.2100 and 0.7900, 0.7300 and 0.2700, 0.7800 and 0.2200, 0.2900 and 0.7100, respectively. AA, AB and BB genotype frequencies were found to be 0.250, 0.212 and 0.538 (P<0.05), 0.451, 0.255 and 0.294 (P<0.05), 0.060, 0.300 and 0.640 (P>0.05), 0.600, 0.260 and 0.140 (P<0.05), 0.700, 0.160 and 0.140 (P<0.05), 0.200, 0.180 and 0.620 (P<0.05) respectively. When all breeds were taken into consideration, A and B allel frequencies were found to be 0.4901 and 0.5099, and AA, AB and BB genotype frequencies were found to be 0.376, 0.228 and 0.396 (P<0.05). It was determined that population was in not Hardy-Weinberg equilibrium (P<0.05). GH-1 gene HaeIII restriction enzymes results were identified two alleles (A and B) and three genotypes (AA, BB and AB). A and B allel frequencies for Hair, Angora, Honamlı, Aleppo, Saanen and Kilis breeds were found to be 0.4038 and 0.5962, 0.4314 and 0.5686, 0.4600 and 0.5400, 0.4500 and 0.5500, 0.3800 and 0.6200, 0.5400 and 0.4600, respectively. AA, AB and BB genotype frequencies were found to be 00.019, 0.769 and 0.212 (P<0.05), 0.235, 0.392 and 0.32 (P>0.05), 0.080, 0.760 and 0.160 (P<0.05), 0.060, 0.780 and 0.160 (P<0.05), 0.160, 0.440 and 0.400 (P>0.05), 0.160, 0.760 and 0.080 (P<0.05), respectively. When all breeds were taken into consideration, A and B allel frequencies were found to be 0.4439 and 0.5561, and AA, AB and BB genotype frequencies were found to be 0.119, 0.650 and 0.231 (P<0.05). It was determined that the population studied was not in Hardy-Weinberg equilibrium (P<0.05).
Açıklama
Anahtar Kelimeler
Pit-1, IGF-1, GH-1, PstI polimorfizmi, Keçi, PstI polymorphism, Goat
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Ouchar, M.A.A. (2019). Türkiye'de Yetiştirilen Bazı Keçi Irklarında Büyüme Hormonu Geni (GH-1), İnsülin Benzeri Büyüme Faktörleri (IGF-1) ve Pitüiter Spesifik Transkripsiyon Faktörü-1 (PİT-1) Genleri Polimorfizmleri. ( Yüksek Lisans Tezi). Selçuk Üniversitesi, Fen Bilimleri Enstitüsü, Konya.
