Differential identification of cattle Sarcocystis spp. by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

Basit Öğe Kaydı
dc.contributor.authorGüçlü, Feyzullah
dc.contributor.authorAldemir, Osman Selçuk
dc.contributor.authorGüler, Leyla
dc.date.accessioned2020-03-26T16:48:11Z
dc.date.available2020-03-26T16:48:11Z
dc.date.issued2004
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractMorphological identification of Sarcocystis spp. and the diagnosis of their developmental stages within the host is very difficult. However, recent developments in molecular biology have produced a number of techniques to overcome these difficulties. In this study, the RAPD-PCR (random amplified polymorphic DNA-PCR) technique was used to investigate the molecular biology of Sarcocystis spp. in cattle. In order to isolate the parasite, phenol extraction/ethanol precipitation method was employed to obtain parasitic DNA. After optimisation of the PCR conditions, five primers were selected to amplify the DNA of each Sarcocystis spp. As a result, various Sarcocystis spp. were detected and differentiated using this RAPD-PCR assay in cattle.en_US
dc.identifier.endpage444en_US
dc.identifier.issn0035-1555en_US
dc.identifier.issue08.09.2020en_US
dc.identifier.startpage440en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/18998
dc.identifier.volume155en_US
dc.identifier.wosWOS:000224975000007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherECOLE NATIONALE VETERINAIRE TOULOUSEen_US
dc.relation.ispartofREVUE DE MEDECINE VETERINAIREen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectsarcocystisen_US
dc.subjectdetectionen_US
dc.subjectdifferential identificationen_US
dc.subjectRAPD-PCRen_US
dc.titleDifferential identification of cattle Sarcocystis spp. by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu