Superoxide dismutase and antioxidant status of hens’ granulosa cells exposed to lead and molybdenum
Yükleniyor...
Dosyalar
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Ünivesitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Mevcut araştırmanın amacı in vitro kültüre edilen tavuk ovaryum granulosa hücrelerine kurşun (Pb) ve molibden (Mo) uygulaması sonrasında superoksid dismutaz (SOD) ve total antioksidan durum (TAS)’un belirlenmesidir. Gereç ve Yöntem: Ovaryum granulosa hücreleri farklı dozlarda kurşun asetat/amonyum molibdat uygulaması ile 18 saat inkübe edildi. Kurşun asetat/amonyum molibdat ise faklı dozlarda uygulandı; grup A (0.09/0.09 mg/mL), grup B (0.13/0.13 mg/mL), grup C (0.17/0.17 mg/mL), grup D (0.33/0.33 mg/mL), grup E (0.5/0.5 mg/mL) ve kontrol. Sonuçlar: Granulosa hücrelerinin antioksidan durumu Pb ve Mo dozuna bağlı olarak değişti. Her iki metalin yüksek dozu SOD aktivitesinde önemli düşmelere neden oldu. Hücrelerin TAS’u deneme gruplarında düşük belirlendi. Öneri: İz elementler hayvanların üreme sistemi üzerine doğrudan veya dolaylı olarak oksitatif strese neden olarak olumsuz etkilere neden olabilir.
Aim: The aim of this study was to determine the activity of superoxide dismutase (SOD) and total antioxidant status (TAS) of ovarian granulosa cells of hens cultured in vitro after lead (Pb) and molybdenum (Mo) administrations. Material and Methods: Ovarian granulosa cells were incubated with lead acetate/ammonium molybdate administrations as follows: group A (0.09/0.09 mg/mL), group B (0.13/0.13 mg/mL), group C (0.17/0.17 mg/mL), group D (0.33/0.33 mg/mL), group E (0.5/0.5 mg/mL) and the control group without any additions for 18 h. Results: Antioxidant status of granulosa cells was dependent on Pb and Mo doses. Higher doses of both metals caused significant reduction of SOD activity. TAS of cells was decreased in experimental groups. Conclusion: Trace elements can adversely affect animal reproductive system and its functions, through either direct or indirect effects on oxidative stress induction.
Aim: The aim of this study was to determine the activity of superoxide dismutase (SOD) and total antioxidant status (TAS) of ovarian granulosa cells of hens cultured in vitro after lead (Pb) and molybdenum (Mo) administrations. Material and Methods: Ovarian granulosa cells were incubated with lead acetate/ammonium molybdate administrations as follows: group A (0.09/0.09 mg/mL), group B (0.13/0.13 mg/mL), group C (0.17/0.17 mg/mL), group D (0.33/0.33 mg/mL), group E (0.5/0.5 mg/mL) and the control group without any additions for 18 h. Results: Antioxidant status of granulosa cells was dependent on Pb and Mo doses. Higher doses of both metals caused significant reduction of SOD activity. TAS of cells was decreased in experimental groups. Conclusion: Trace elements can adversely affect animal reproductive system and its functions, through either direct or indirect effects on oxidative stress induction.
Açıklama
Anahtar Kelimeler
Granulosa hücreleri, total antioksidan durum, Granulosa cells, lead, kurşun, molibden, molybdenum, SOD, total antioxidant status
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
28
Sayı
4
Künye
Capcarova, M., Kolesarova, A., Sirotkin, A. V., (2012). Superoxide dismutase and antioxidant status of hens’ granulosa cells exposed to lead and molybdenum. Eurasian Journal of Veterinary Sciences, 28 (4). 209-213.
