A comparison of immuncapture agglutination and ELISA methods in serological diagnosis of brucellosis

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dc.contributor.authorÖzdemir M.
dc.contributor.authorFeyzio?lu B.
dc.contributor.authorKurto?lu M.G.
dc.contributor.authorDo?an M.
dc.contributor.authorDa?ï H.T.
dc.contributor.authorYüksekkaya S.
dc.contributor.authorKeşli R.
dc.date.accessioned2020-03-26T18:22:07Z
dc.date.available2020-03-26T18:22:07Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and immuncapture agglutination tests with Coombs anti-brucella test. Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were included into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Immuncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated. Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test. Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis. © Ivyspring International Publisher.en_US
dc.identifier.doi10.7150/ijms.8.428en_US
dc.identifier.endpage432en_US
dc.identifier.issn1449-1907en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage428en_US
dc.identifier.urihttps://dx.doi.org/10.7150/ijms.8.428
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27157
dc.identifier.volume8en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherIvyspring International Publisheren_US
dc.relation.ispartofInternational Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBrucellosisen_US
dc.subjectELISAen_US
dc.subjectIgGen_US
dc.subjectIgMen_US
dc.subjectImmuncaptureen_US
dc.subjectSerologic diagnosisen_US
dc.titleA comparison of immuncapture agglutination and ELISA methods in serological diagnosis of brucellosisen_US
dc.typeArticleen_US

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