The expression of steroidogenic genes in ovine corpus luteum during early pregnancy
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Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın amacı erken gebelikte koyun korpus luteumunda steroidojenik genlerinin ekspresyonunu araştırmaktır. Gereç ve Yöntem: Çalışmanın hayvan modelinde gebe koyunlar üç alt grup olacak şekilde dizayn edilmiştir; gebelik 12: G12, gebelik 16: G16 ve gebelik 22: G22. gün ve siklik 16. gün (S16). Steroidojenik genlerin ekspresyon seviyesi (StAR, 3?HSD, P450scc) kantitatif polimeraz zincir reaksiyonu (PZR) ile değerlendirilmiştir ve StAR mRNA lokalizasyonu gebelik 16. günde siklik 16. güne karşı in situ hibridizasyon yöntemi ile gösterilmiştir. Bulgular: Gebelik günlerinde (G16 ve G22) StAR mRNA ekspresyonu G12 ile kıyaslandığında daha fazla olmasına (p<0.05) karşın, G16 ve G22 günleri arasında fark bulunmamaktadır (p>0.05). Benzer olarak, P450scc mRNA ekspresyon seviyesi gebelik günleri G22 ve G16’da G12’den fazla olarak bulunmuştur (p<0.05). Ancak, P450scc mRNA ekspresyonunda G16 ve G22 günleri arasında fark bulunmamaktadır (p>0.05). Benzer olarak, 3?HSD mRNA ekspresyon seviyesi gebelik günleri G22 ve G16’da G12’den daha fazladır (p<0.05). Ancak, 3?HSD mRNA ekspresyonunda G16 ve G22 günleri arasında değişiklik tespit edilememiştir (p>0.05). Bu çalışmadan, in situ hibridizasyonda, S16 günde StAR mRNA’sı luteal hücrelerde bulunamadı. Buna rağmen, luteal hücrelerde G16 günde StAR mRNA tespit edildi. Öneri: Steroidojenik yolakta bulunan genlerin ekspresyon seviyesinin koyunlarda erken gebelikte kritik bir rol oynadığı görülmüştür. Sonuç olarak, koyunların korpus luteumundaki steroidojenik yolağın, erken gebeliğin devamlılığı için gerekli progesteron sentezini transkripsiyonel olarak düzenleyebileceği önerilebilir.
Aim: The goal of this study was to investigate the expression of steroidogenic genes in ovine corpus luteum during early pregnancy Materials and Methods: The animal model was designed as pregnancy; ewes were divided into three sub-groups, pregnancy 12th day: P12, pregnancy 16th day: P16, pregnancy 22th day: P22, and cyclic day 16 (C16). The expression of steroidogenic genes (steroidogenic acute regulatory protein; StAR, cytochrome P450 side-chain cleavage; P450sscc, and 3b-hydroxysteroid dehydrogenase/delta5 delta4-isomerase; 3?HSD) was evaluated using qPCR, and mRNA localization of StAR was detected on P16 against C16 through in-situ hybridization. Results: The expression of StAR mRNA was higher on day P22 and P16 compared to P12 (p<0.05), while it was at a steady-state level on day P22 vs P16 (p>0,05). mRNA expression of P450scc was greater on day P22 and P16 than day P12 (p<0.05), however on day P22 vs P16, it was at a steady-state level (p>0,05). Also, mRNA expression of 3?HSD had a similar trend; it was higher on day P22 and P16 compared to P12 (p<0.05), while it was at a steady-state level on day P22 vs P16 (p>0,05). In in-situ hybridization, we did not detect StAR mRNA on cyclic day 16 (C16) while abundantly expressed in luteal cells in P16. Conclusion: The mRNA expression of steroidogenic genes may appear to play a critical role during early pregnancy in ewes. Accordingly, it can be suggested that the steroidogenic pathway in the corpus luteum of ewe may transcriptionally regulate progesterone synthesis required for maintenance of early pregnancy
Aim: The goal of this study was to investigate the expression of steroidogenic genes in ovine corpus luteum during early pregnancy Materials and Methods: The animal model was designed as pregnancy; ewes were divided into three sub-groups, pregnancy 12th day: P12, pregnancy 16th day: P16, pregnancy 22th day: P22, and cyclic day 16 (C16). The expression of steroidogenic genes (steroidogenic acute regulatory protein; StAR, cytochrome P450 side-chain cleavage; P450sscc, and 3b-hydroxysteroid dehydrogenase/delta5 delta4-isomerase; 3?HSD) was evaluated using qPCR, and mRNA localization of StAR was detected on P16 against C16 through in-situ hybridization. Results: The expression of StAR mRNA was higher on day P22 and P16 compared to P12 (p<0.05), while it was at a steady-state level on day P22 vs P16 (p>0,05). mRNA expression of P450scc was greater on day P22 and P16 than day P12 (p<0.05), however on day P22 vs P16, it was at a steady-state level (p>0,05). Also, mRNA expression of 3?HSD had a similar trend; it was higher on day P22 and P16 compared to P12 (p<0.05), while it was at a steady-state level on day P22 vs P16 (p>0,05). In in-situ hybridization, we did not detect StAR mRNA on cyclic day 16 (C16) while abundantly expressed in luteal cells in P16. Conclusion: The mRNA expression of steroidogenic genes may appear to play a critical role during early pregnancy in ewes. Accordingly, it can be suggested that the steroidogenic pathway in the corpus luteum of ewe may transcriptionally regulate progesterone synthesis required for maintenance of early pregnancy
Açıklama
Anahtar Kelimeler
Korpus luteum, Erken gebelik, P450scc, StAR, 3βHSD, Corpus luteum, Early pregnancy
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
37
Sayı
2
Künye
Hitit, M., Köse, M., Bülbül, B., Atlı, M. O., Koçak, N., (2021). The expression of steroidogenic genes in ovine corpus luteum during early pregnancy. Eurasian Journal of Veterinary Sciences, 37 (2), 93-100. DOI: 10.15312/EurasianJVetSci.2021.331
