Comparison of five different rna isolation methods from equine endometrium for gene transcription analysis

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dc.contributor.authorKurar E.
dc.contributor.authorAtli M.O.
dc.contributor.authorGüzeloğlu A.
dc.contributor.authorÖzşensoy Y.
dc.contributor.authorSemacan A.
dc.date.accessioned2020-03-26T18:05:39Z
dc.date.available2020-03-26T18:05:39Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Zootekni ve Hayvan Besleme Bölümüen_US
dc.description.abstractIn this study, five different isolation protocols to extract total RNA from biopsies of equine endometrium were compared in terms of quality and quantity of RNA samples with respect to downstream gene transcription analysis, such as Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Three phenol-chloroform based protocols (TRIzol, TRItidy, EZ-RNA) and two column based protocols (UltraClean™ and E.Z.N.A.®) that were commercially available were used. Each protocol yielded good quality total RNA and distinct 28S and 18S rRNA bands were observed in agarose gel electrophoreses. Amount of total RNA isolated was lower for EZ-RNA protocol. Column based protocols had RNA contaminated with great amount of genomic DNA, however, DNAse-I digestion was able to fully clean the DNA contamination from RNA in all the protocols used. Following cDNA synthesis and PCR, GAPDH, a housekeeping gene, bands were amplified from all the samples. In conclusion, all the protocols used extracted good quality but different amounts of total RNA and it is strongly recommended that RNA samples must undergo DNAse-I digestion before RT-PCR to eliminate gDNA contamination.en_US
dc.identifier.citationKurar, E., Atlı, M. O., Güzeloğlu, A., Özşensoy, Y., Semacan, A. (2010). Comparison of Five Different RNA İsolation Methods from Equine Endometrium for Gene Transcription Analysis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(5), 851-855.
dc.identifier.endpage855en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage851en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/25494
dc.identifier.volume16en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKurar E.
dc.institutionauthorAtli M.O.
dc.institutionauthorGüzeloğlu A.
dc.institutionauthorÖzşensoy Y.
dc.institutionauthorSemacan A.
dc.language.isotren_US
dc.publisherVeteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEquine endometriumen_US
dc.subjectRNA isolationen_US
dc.subjectRT-PCRen_US
dc.titleComparison of five different rna isolation methods from equine endometrium for gene transcription analysisen_US
dc.typeArticleen_US

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