KAN KÜLTÜRLERİNDEN İZOLE EDİLEN KOAGÜLAZ NEGATİF STAFİLOKOKLARIN TÜR TAYİNİ VE ANTİBİYOTİKLERE DİRENÇ ORANLARI
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Koagülaz negatif stafilokoklar insan derisinde mikrobiyota elemanı olarak bulunurlar. Bu bakteriler son yıllarda çok sayıda hasta örneğinden izole edilmekte ve bazılarında etken olarak kabul edilmektedirler. Antibiyotik kullanımına bağlı seçici baskıdan dolayı özellikle stafilokoklarda birçok antibiyotiğe karşı direnç gelişmiştir. Bu çalışmanın amacı kan kültürlerinden izole edilen koagülaz negatif stafilokokların tür düzeyinde tanımlanması ve antibiyotik direnç oranlarının araştırılmasıdır. Mikrobiyoloji laboratuvarına 1 Haziran 2013-31 Mayıs 2014 tarihleri arasında çeşitli kliniklerden gönderilen kan kültürleri BACTEC otomatize kan kültür cihazında takip edilmiştir. Üreme sinyali veren şişelerden Gram boyama yapılmış ve % 5 koyun kanlı Columbia agar ve Eozin Metilen Mavisi Agar besiyerlerine pasajlanmıştır. Besiyerinde üreyen Gram pozitif, katalaz pozitif ve koagülaz negatif bakterilerin tür tayini ve antibiyotik duyarlılık testi VITEK 2 (bioMérieux, Fransa) otomatize sistemi kullanılarak yapılmıştır. Toplam 236 koagülaz negatif stafilokok suşunun % 49u Staphylococcus epidermidis, % 42si Staphylococcus hominis, % 9u Staphylococcus haemolyticus olarak tanımlanmıştır. Tüm suşlarda metisiline direnç oranı % 82 olarak saptanmıştır. Vankomisin, teikoplanin, daptomisin, tigesiklin ve linezolide direnç tespit edilmemiştir. Penisiline % 95, eritromisine % 81, tetrasikline % 66, klindamisine % 61, gentamisine % 42, levofloksasine % 38, moksifloksasine % 35 ve trimetoprim- sülfametoksazole % 20 oranında direnç saptanmıştır. Koagülaz negatif stafilokokların antimikrobiyal ajanlara direnç oranları yıllar içerisinde değişiklik göstermektedir. Bu bakterilerde artan direnç oranlarının izlenmesi antibiyotik kullanım politikalarının belirlenmesine katkı sağlayacaktır.
Coagulase-negative staphylococci are found in the microbiota of human skin. These bacteria have been isolated from many patientssamples and some of them have been accepted as causative agents. Due to the selective pressure related to anti- biotic use, especially staphylococci have developed resistance to multiple antibiotics. The aim of this study is identification of coagulase-negative staphylococci isolated from blood cultures to species level and to investigate antibiotic resistance rates. Blood cultures were sent from various clinics to the microbiology laboratory between 1 June 2013-31 May 2014 were continuously monitored by the BACTEC automated blood culture system. Preparations from the bottles giving positive signals were stained by Gram method and subcultured to Columbia agar with 5 % sheep blood and Eosin Methylene Blue agar. The identification and antibiotic susceptibility tests of bacteria that grew on these media and which were Gram positive, catalase positive and coagulase negative were carried out with VITEK 2 (bioMérieux, France) automated system. A total of 236 coagulase negative staphylococci strains were detected; 49 % were identified as Staphylococcus epider- midis, 42 % Staphylococcus hominis and 9 % Staphylococcus haemolyticus. Methicillin resistance rate of all strains was 82 %. All strains were susceptible to vancomycin, daptomycin, tigecycline and linezolid. Antibiotic resistance rates of strains were 95 % for penicillin, 81 % erythromycin, 66 % tetracycline, 61 % clindamycin, 42 % gentamicin, 38 % levofloxacin, 35 % moxifloxacin and 20 % trimethoprim-sulfamethoxazole. Antimicrobial resistance rates of coagulase negative staphylococci have been changing in recent years. Monitoring increasing resistance rates will contribute to determination of antibiotic usage policies.
Coagulase-negative staphylococci are found in the microbiota of human skin. These bacteria have been isolated from many patientssamples and some of them have been accepted as causative agents. Due to the selective pressure related to anti- biotic use, especially staphylococci have developed resistance to multiple antibiotics. The aim of this study is identification of coagulase-negative staphylococci isolated from blood cultures to species level and to investigate antibiotic resistance rates. Blood cultures were sent from various clinics to the microbiology laboratory between 1 June 2013-31 May 2014 were continuously monitored by the BACTEC automated blood culture system. Preparations from the bottles giving positive signals were stained by Gram method and subcultured to Columbia agar with 5 % sheep blood and Eosin Methylene Blue agar. The identification and antibiotic susceptibility tests of bacteria that grew on these media and which were Gram positive, catalase positive and coagulase negative were carried out with VITEK 2 (bioMérieux, France) automated system. A total of 236 coagulase negative staphylococci strains were detected; 49 % were identified as Staphylococcus epider- midis, 42 % Staphylococcus hominis and 9 % Staphylococcus haemolyticus. Methicillin resistance rate of all strains was 82 %. All strains were susceptible to vancomycin, daptomycin, tigecycline and linezolid. Antibiotic resistance rates of strains were 95 % for penicillin, 81 % erythromycin, 66 % tetracycline, 61 % clindamycin, 42 % gentamicin, 38 % levofloxacin, 35 % moxifloxacin and 20 % trimethoprim-sulfamethoxazole. Antimicrobial resistance rates of coagulase negative staphylococci have been changing in recent years. Monitoring increasing resistance rates will contribute to determination of antibiotic usage policies.
Açıklama
Anahtar Kelimeler
Farmakoloji ve Eczacılık
Kaynak
ANKEM Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
30
Sayı
1
