Impact of Deformed Wing Virus Master Variants (DWV-A, DWV-B, and DWV-C) in Managed Honey Bee Colonies of Türkiye
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Dosyalar
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada, Türkiye’nin İç Anadolu ve Akdeniz Bölgelerinde yer alan bal arısı kovanlarında deforme kanat virüsü (DWV) ana varyantlarının belirlenmesi amaçlandı. Ayrıca arı kovanlarında sirküle olan DWV genotiplerinin bal arısı kovanlarında gözlenen klinik belirtilerle ilişkisi araştırıldı. Gereç ve Yöntem: Bu çalışma için Aksaray, Isparta, Karaman, Konya ve Niğde illerinden 2019 yılı ilkbahar-yaz ve sonbahar sezonlarında aynı 25 kovandan yetişkin bal arıları toplandı. DWV'ye özgü nükleik asit ve DWV genotipleri, sırasıyla DWV gerçek zamanlı RT-PCR tahlili ve ABC tahlili ile tespit edildi. Bulgular: DWV enfeksiyonu örnekleme yapılan her mevsimde tespit edildi. Örneklenen bir çok kolonide klinik bulgu görülmezken, bazı arılıklarda kanatlarda şekil bozukluğu, titreme, felç, karında şişlik, verim kaybı ve ölü arılar gözlemlendi. Erişkin bal arılarında DWV-A, DWV-B ve DWV-C yaygınlıkları sırasıyla %62, %82 ve %24 idi. Arı kovanlarında tespit edilen baskın genotip, DWV-B ana varyantıydı (%98). Ayrıca DWV-A ana varyantının virüs yükü, kışlama kayıpları görülen bal arısı kovanlarının tamamında yüksekti. Öneri: Bu çalışmada, Türkiye’de sirküle olan DWV ana varyantlarının mevcut durumu ve bal arısı kolonileri üzerindeki etkileri ile ilgili veriler ilk kez rapor edildi. Böylece Türkiye arı kovanlarında yılın her mevsiminde değişen oranlarda verim kayıplarına neden olan DWV’nin dikkatle izlenmesi gerektiği önerilmektedir.
Aim: This study aimed to determine the deformed wing virus (DWV) master variants in managed honey bee hives in Central Anatolia and the Mediterranean Regions of Türkiye. Also, the relationship of DWV genotypes circulating in the apiaries with clinical signs observed in honey bee hives was investigated. Materials and Methods: For this study, adult honey bees were collected from the same 25 hives in the spring-summer and autumn seasons of 2019 from the provinces of Aksaray, Isparta, Karaman, Konya and Nigde. DWV-specific nucleic acid and DWV genotypes were detected by DWV real-time RT-PCR assay and ABC assay, respectively. Results: Deformed wing virus infection was detected in each sampling season. While many colonies were without any clinical signs, in some of the apiaries where samples were collected, wing deformity, trembling, paralysis, swelling in the abdomen, loss of productivity, and dead bees were observed. The prevalences of DWV-A, DWV-B, and DWV-C in adult honey bees were 62%, 82%, and 24%, respectively. The dominant genotype detected in bee hives was the DWV-B master variant (98%). Also, the virus load of the DWV-A master variant was high in all of the honey bee hives with wintering losses. Conclusion: In this present study, data on the current status of DWV master variants circulating in Turkey and their impacts on honey bee colonies are reported for the first time. Thus, it is thought that DWV, which causes yield losses at varying rates in every season of the year in Turkish bee hives, should be carefully monitored.
Aim: This study aimed to determine the deformed wing virus (DWV) master variants in managed honey bee hives in Central Anatolia and the Mediterranean Regions of Türkiye. Also, the relationship of DWV genotypes circulating in the apiaries with clinical signs observed in honey bee hives was investigated. Materials and Methods: For this study, adult honey bees were collected from the same 25 hives in the spring-summer and autumn seasons of 2019 from the provinces of Aksaray, Isparta, Karaman, Konya and Nigde. DWV-specific nucleic acid and DWV genotypes were detected by DWV real-time RT-PCR assay and ABC assay, respectively. Results: Deformed wing virus infection was detected in each sampling season. While many colonies were without any clinical signs, in some of the apiaries where samples were collected, wing deformity, trembling, paralysis, swelling in the abdomen, loss of productivity, and dead bees were observed. The prevalences of DWV-A, DWV-B, and DWV-C in adult honey bees were 62%, 82%, and 24%, respectively. The dominant genotype detected in bee hives was the DWV-B master variant (98%). Also, the virus load of the DWV-A master variant was high in all of the honey bee hives with wintering losses. Conclusion: In this present study, data on the current status of DWV master variants circulating in Turkey and their impacts on honey bee colonies are reported for the first time. Thus, it is thought that DWV, which causes yield losses at varying rates in every season of the year in Turkish bee hives, should be carefully monitored.
Açıklama
Anahtar Kelimeler
Bal arısı, DWV ana varyantları, gerçek zamanlı RT-PCR, klinik belirtiler, Clinical signs, DWV master variants, honey bee, real-time RT-PCR
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
39
Sayı
3
Künye
Öz, M. E., Avcı, O., Doğan, M., (2023). Impact of deformed wing virus master variants (DWV-A, DWV-B, and DWV-C) in
managed honey bee colonies of Türkiye. Eurasian Journal of Veterinary Sciences, 39(3), 124-131.
DOI: 10.15312/EurasianJVetSci.2023.407
