Investigation of enteric bovine coronavirus infections in calves and the role of clinically healthy cattle in epidemiology of coronavirus infections
Küçük Resim Yok
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Enterik bovine coronavirus (BCV) varlığı yönünden; 63 adet ishalli buzağıdan ve 77 adet klinik olarak sağlıklı inekten (63 adedi ishalli buzağıların annelerine ait olmak üzere) alman dışkı örnekleri ELISA ile incelendi. BCV; araştırmada kullanılan hiçbir annenin/ineğin dışkısında saptanamazken, 63 buzağıya ait dışkının 2 (% 3.1) tanesinde tespit edildi. Ayrıca, 63 ishalli buzağının 36'sından ve 77 ineğin 46'sından alınan kan serum örnekleri kompetetif-ELISA ile coronavimsa spesifik antikorlar yönünden araştırıldı. Sonuçta 36 buzağının 9 adedinde (% 25) ve 46 ineğin 35 adedinde (% 76) seropozitiflik gözlendi. Virus izolasyonu amacıyla BCV antijen varlığı saptanan 2 adet dışkı örneğinin Vero, MDBK ve HRT-18 hücre kültürlerine ekimleri yapıldı. Dışkı örneklerinin her bir hücre kültürüne 5 kör pasajı sonucunda BCV izolasyonu gerçekleştirilemedi.
Fecal specimens from 63 calves and 77 healthy cows (Out of 77 cows, 63 were dams of calves) were examined for the presence of bovine coronavirus (BCV) antigens by ELISA. Coronavirus was detected in 2 (3.1%) of 63 calves while not detected in any of the cows. Serum samples from 36 of 63 diarrheic calves and from 46 of 77 cows were also tested for coronavirus specific antibodies by competitive ELISA. Nine (25%) of 36 calves and 35 (76%) of the 46 cows were seropositive. Two fecal samples that were detected to be BCV antigen positive by ELISA were also inoculated into Vero, MDBK and HRT-18 cell cultures. BCV was not isolated from two faecal samples after 5 blind passages on the cell lines.
Fecal specimens from 63 calves and 77 healthy cows (Out of 77 cows, 63 were dams of calves) were examined for the presence of bovine coronavirus (BCV) antigens by ELISA. Coronavirus was detected in 2 (3.1%) of 63 calves while not detected in any of the cows. Serum samples from 36 of 63 diarrheic calves and from 46 of 77 cows were also tested for coronavirus specific antibodies by competitive ELISA. Nine (25%) of 36 calves and 35 (76%) of the 46 cows were seropositive. Two fecal samples that were detected to be BCV antigen positive by ELISA were also inoculated into Vero, MDBK and HRT-18 cell cultures. BCV was not isolated from two faecal samples after 5 blind passages on the cell lines.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Veterinarium
WoS Q Değeri
Scopus Q Değeri
Cilt
18
Sayı
1
