Grafen oksitle muamele edilmiş sağlıklı akciğer Beas-2B hücre hattının antioksidan savunma mekanizması ve inflamasyon süreçlerine bazı turp türlerinin etkisi
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Dosyalar
Tarih
2023
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Yayıncı
Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu tezin amacı, hayatın birçok alanında kullanımı olan grafen oksit nanomalzemesinin zararlı maddelerin temizlenmesine etkilerinin saptanması ve bu özellikleri indirgeyici bir bitkisel kaynaklı kür oluşturmaktır. Bitkisel kaynaklı ana malzeme de referans olarak alınan analizler yapılandırılarak uygulanarak oksidatif stres ve antioksidanlar düzeyde analizler sağlanır. Bu çalışma için sağlıklı akciğer hücre hattı Beas-2B listelenmiştir. Hayatında her alanda bulunabilecek olası grafen oksit ve ebeveynlerinin etkinliğinin ölçülebilmesi açısından yol göstericinin göreceğiyle sağlıklı akciğerleri seçilmiştir. Grafen oksitin iki boyutu (50 nm ve 400 nm) tercih edilmiştir. Yapılan literatür araştırmasında grafen oksitin bozulmasının hücre içine penetrasyon ve hücre içinde tutulduğu sürelerde etkilendiği görülmüştür. Bitkisel kaynaklı kür için Brassicaceae ailesinin üyesi olan Raphanus sativus türü, siyah turp ve karpuz turbu seçilmiştir. Raphanus sativus cinsi beyaz turp (karpuz turbu) dışı beyaz içi pembe renkte yumrukların içindedir. Raphanus sativus türü siyah turp ise yine yumruk büyüklüğünde siyah dışı içi beyaz turp türüdür. Türkiye'de kış aylarında bol miktarda bulunan bu turp çeşitlerinin hücreyi zararlı radikallere karşı koruma ve antiinflamatuar etkinlik gösterdiği görüldü. Turp içinde olduğu HPLC analizleri ile tespit edilmiş olan sulforafan etken maddesi; ticari olarak satın alınıp referans olarak siyah ve beyaz turp öz sularının enzim ve interlökin test kitlerinde kaydedildi. Sulforafan biyoaktif bir fitokimyasal maddedir. Yapılan literatür araştırmalarında sülforafan maddesinin antikanser özellikleri, kanser tedavisi yollarını indirgediği ve çoğalmalarını baskıladığı görülmektedir. Bu nedenle referans madde olarak sülforafan biyoaktif fitokimyasal madde seçilmiştir. Her bir grafen oksit boyutu için ayrı ayrı IC50 değerleri hesaplanmış ve sonrasında sağlıklı akciğerlere bu değerlerin üzerinde, altında ve tam olarak bu değerlerde grafen oksit uygulaması yapılmıştır. GSH-PX düzeyinde yapılana antioksidatif etkinlik için en yüksek değerde siyah turp öz suyunda görülmüştür. Yapılan T-SOD analizinde yine aynı şekilde siyah turp öz suyunun antioksidan özelliği diğer gruplardan daha yüksek düzeydedir. Interlökin-6 testinde arttığının arttığı grafen oksit uygulanan turp öz suyu uygulandı, buna göre daha fazla olduğu ve siyah turp öz suyunun eklenmesi sağlandı. Bu noktada sulforafan ve turp öz sularının anti-inflamatuar özelliği gösterdiği ve üstün enzim geliştirmeyi arttıran grafen oksit miktarı ve boyuta bağlı olarak farklı potansiyelde üretilen reaktif oksijen türlerini indirgediği potansiyele sahiptir.
The aim of this thesis is to determine the effects of graphene oxide nanomaterial, which is used in many areas of life, on the cleansing of harmful substances and to create a herbal cure that reduces these properties. The main material produced from plants also provides resistance to oxidative stress and antioxidants by applying reference tests. The healthy lung cell line Beas-2B was listed for this study. In order to measure the effectiveness of graphene oxide and its parents, which can be found in every area of life, healthy lungs were chosen as the guide would see. Two sizes of graphene oxide (50 nm and 400 nm) were preferred. In the literature research, it was seen that the degradation of graphene oxide would change the penetration into the cell and the storage time in the cell. Since there will be different views in different sizes, it was decided to present the different size distribution in a useful way in order to change and give ideas. This intended graphene oxide was chosen as 50 nm and 400 nm. For the herbal cure, black and white radishes of the Raphanus sativus species, a member of the Brassicaceae family, were selected. White radish (watermelon radish) of the genus Raphanus sativus is in fists that are white on the outside and pink on the inside. Raphanus sativus black radish is a fist-sized radish that is black on the outside and white on the inside. It was decided to use this thesis because these radish varieties, which are abundant in the winter months in Turkey, are useful, show protection against harmful radicals in the body and show anti-inflammatory activity, are considered to be a beneficial food in the literature, and are also low-cost.The active ingredient sulforaphane in radish, which was not detected by HPLC analysis; It was purchased commercially and used as a reference in black and white radish juices and interleukin test kits. Sulforaphane is a bioactive phytochemical. In literature research, it is seen that sulforaphane has anticancer properties, reduces cancer treatment methods and suppresses their proliferation. For this reason, sulforaphane bioactive phytochemical was chosen as the reference substance. IC50 values were calculated separately for each graphene oxide size, and then graphene oxide was applied to healthy lungs above, below and exactly at these values. The highest value for antioxidative activity in GSH-PX level was seen in black radish sap. In the SOD test, the antioxidant properties of black radish sap were found to be higher than other groups. Since the increase in the interleukin-6 test when applied to graphene oxide was higher than when applied to general radish sap, black radish sap was applied. At this point, sulforaphane and radish juices have strong anti-inflammatory properties.
The aim of this thesis is to determine the effects of graphene oxide nanomaterial, which is used in many areas of life, on the cleansing of harmful substances and to create a herbal cure that reduces these properties. The main material produced from plants also provides resistance to oxidative stress and antioxidants by applying reference tests. The healthy lung cell line Beas-2B was listed for this study. In order to measure the effectiveness of graphene oxide and its parents, which can be found in every area of life, healthy lungs were chosen as the guide would see. Two sizes of graphene oxide (50 nm and 400 nm) were preferred. In the literature research, it was seen that the degradation of graphene oxide would change the penetration into the cell and the storage time in the cell. Since there will be different views in different sizes, it was decided to present the different size distribution in a useful way in order to change and give ideas. This intended graphene oxide was chosen as 50 nm and 400 nm. For the herbal cure, black and white radishes of the Raphanus sativus species, a member of the Brassicaceae family, were selected. White radish (watermelon radish) of the genus Raphanus sativus is in fists that are white on the outside and pink on the inside. Raphanus sativus black radish is a fist-sized radish that is black on the outside and white on the inside. It was decided to use this thesis because these radish varieties, which are abundant in the winter months in Turkey, are useful, show protection against harmful radicals in the body and show anti-inflammatory activity, are considered to be a beneficial food in the literature, and are also low-cost.The active ingredient sulforaphane in radish, which was not detected by HPLC analysis; It was purchased commercially and used as a reference in black and white radish juices and interleukin test kits. Sulforaphane is a bioactive phytochemical. In literature research, it is seen that sulforaphane has anticancer properties, reduces cancer treatment methods and suppresses their proliferation. For this reason, sulforaphane bioactive phytochemical was chosen as the reference substance. IC50 values were calculated separately for each graphene oxide size, and then graphene oxide was applied to healthy lungs above, below and exactly at these values. The highest value for antioxidative activity in GSH-PX level was seen in black radish sap. In the SOD test, the antioxidant properties of black radish sap were found to be higher than other groups. Since the increase in the interleukin-6 test when applied to graphene oxide was higher than when applied to general radish sap, black radish sap was applied. At this point, sulforaphane and radish juices have strong anti-inflammatory properties.
Açıklama
Anahtar Kelimeler
Antioksidan Grafen, Sülforafan, Raphanus sativus L., Antioxidant Graphene, Sulforaphan
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Yılmaz, K. (2023). Grafen oksitle muamele edilmiş sağlıklı akciğer Beas-2B hücre hattının antioksidan savunma mekanizması ve inflamasyon süreçlerine bazı turp türlerinin etkisi. (Yüksek Lisans Tezi). Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, Konya.
