The correlation between neopterin, myeloperoxidase and Oxidative DNA damage in sheep with natural babesiosis
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışma doğal babesiozisli koyunlarda Neopterin, MPO ve DNA ha sarı arasında bir ilişki olup olmadığının belirlenmesi amacıyla planlandı.
Gereç ve Yöntem: Çalışmaya 1-3 yaşlarında Akkaraman 20 sağlıklı koyun (Kontrol) ve 20 doğal babesiozisli (Hasta) olmak üzere 40 koyun dahil edildi. Tüm çalışma gruplarından V. Jugularis’ten usulüne uygun biyokimya tüplerine kan örnekler alındı. Yapılan froti sonucuna göre etken belirlenen hayvanlarda ve ayrıca hastalık etkeni PCR sonucuna göre doğrulandı. Sağlıklı ve hastalıklı hayvanlardan elde edilen serumlardan Neopterin, MPO ve oksidatif DNA ha sarı (8-OHdG) ELISA methodu kullanılarak belirlendi.
Bulgular: Neopterin ve 8-OHdG seviyeleri, kontrol grubunda hasta grubuna kıyasla anlamlı derecede daha düşük tespit edildi (P0.05)
Öneri: Bu çalışmada, babesiosisli koyunlarda immünolojik yanıtın göstergeleri olan neopterin ve oksidatif DNA hasarında önemli bir artış gözlemlendi. Özellikle neopterin, babesiosis'e karşı immünolojik yanıtın önemli bir belirteci olarak düşünülebilir.
Aim: The present study was designed to investigate the correlation between neopterin, myeloperoxidase (MPO), and DNA damage in sheep naturally infected with babesiosis. Materials and Methods: The study included 40 sheep Akkaraman, 1-3 years aged, with 20 healthy individuals (control group) and 20 naturally infected with babesiosis (patient group). Blood samples were collected from the jugular vein into biochemical tubes from all sheep. The cause of the disease was confirmed through smear tests and PCR analysis. Neopterin, myeloperoxidase (MPO), and oxidative DNA damage (8-OHdG) levels were measured using an ELISA method. Results: Neopterin and 8-OHdG levels were significantly lower in the control group compared to the patient group (P0.05) Conclusion: In this study, a significant increase in neopterin and oxidative DNA damage, which are indicators of an immunological response, was observed in sheep with babesiosis. Neopterin in particular can be considered as an important marker of the immunological response to babesiosis.
Aim: The present study was designed to investigate the correlation between neopterin, myeloperoxidase (MPO), and DNA damage in sheep naturally infected with babesiosis. Materials and Methods: The study included 40 sheep Akkaraman, 1-3 years aged, with 20 healthy individuals (control group) and 20 naturally infected with babesiosis (patient group). Blood samples were collected from the jugular vein into biochemical tubes from all sheep. The cause of the disease was confirmed through smear tests and PCR analysis. Neopterin, myeloperoxidase (MPO), and oxidative DNA damage (8-OHdG) levels were measured using an ELISA method. Results: Neopterin and 8-OHdG levels were significantly lower in the control group compared to the patient group (P0.05) Conclusion: In this study, a significant increase in neopterin and oxidative DNA damage, which are indicators of an immunological response, was observed in sheep with babesiosis. Neopterin in particular can be considered as an important marker of the immunological response to babesiosis.
Açıklama
Anahtar Kelimeler
Babesiosis, DNA Hasarı, Koyun, Miyeloperoksidaz, Neopterin, DNA Damage, Myeloperoxidase, Sheep
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
40
Sayı
4
Künye
Cetin et al 2024. The correlation between neopterin, myeloperoxidase and oxidative DNA damage in sheep with natural babesiosis Eurasian J Vet Sci, 40, 4, 161-166. 10.15312/EurasianJVetSci.2024.441
