Hipertansif hemodiyaliz hastalarında mi'RNA'ların fonksiyonu ve mi'RNA'ların hipertansiyon patogenezindeki rolü
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi, Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: HT dünyadaki insan nüfusunun 1 milyardan fazlasını etkileyen kardiyovasküler hastalıkların ana risk faktörüdür. Son dönemlerde kronik hastalıkların tedavilerinde yeni araştırma türleri içinde en yaygın olanı miRNA aracılı araştırmalardır. Fonksiyonları birçok hastalıkta (kanser, metabolik sendromlar ve diyabet) iyi bilinse de kalp damar hastalıkları ve HT ile ilişkili bulunan rolleri çok azdır. Güncel çalışmalar miRNA'ların HT'nin etyoloji ve karışık patogenezinde büyük fonksiyonları olabileceğinin düşündürür niteliktedir. Çok sayıda çalışma, mikroRNA'ların hücresel proliferasyon, apoptoz, farklılaşma ve hastalıkların gelişimi dahil olmak üzere çeşitli hücresel işlemlerde yer aldığını göstermiştir. miRNA'lar transkripsiyon sonrası seviyede gen ekspresyonunu düzenleyebilir, birçok tümör tipinde ve kardiyovasküler sistemde eksprese edilir ve kardiyovasküler hastalıkların oluşumunda ve gelişiminde önemli bir rol oynar. Bu araştırma, 50 hipertansif hemodiyaliz hastasında miRNA'ların fonksiyonu, değişen seviyeleri ve mi'RNA'ların hipertansiyon patogenezindeki rolünü gözden geçirmeyi planlamıştır. Gereç ve yöntem: Çalışma Selçuk Üniversitesi Tıp Fakültesi Etik Kurulu'nun 16.10.2019 tarihli ve 2019/269 sayılı kararı ile onaylandı. Bu çalışma Selçuk Üniversitesi Tıp Fakültesi İç hastalıkları A.D.Nefroloji bölümü, Tıbbi Genetik A.D ve Hemodiyaliz ünitesinde 01.05.2020-07.05.2020 tarihleri arasında prospektif olarak gerçekleştirildi. Tüm veriler SPSS 21.0 istatistik paket programı kullanılarak değerlendirildi. Verilerin hesaplanmasında sayı, yüzde, ortalama, standart sapma gibi ölçütler kullanıldı. Sayısal değişkenler arasındaki ilişkiyi değerlendirmek için Pearson korelasyon analizlerinden yararlanıldı. Anlamlılık düzeyi p<0,005 alındı. Bulgular: Tüm gruplar üzere bakıldığında miRNA-370 kontrol grubunda, miRNA-758 ise hasta grubunda anlamlı bulundu. miRNA-33 ile miRNA-370 ve miRNA-335 ile miRNA-758 arasında istatistiksel olarak anlamlı ilişki bulundu. miRNA-758 ile SKB ve DKB arasında, miRNA-33 ile fosfor arasında istatiksel olarak anlamlı ilişki bulundu. miRNA-335 ile fosfor ve PTH arasında, miRNA-758 ile Na ve PLT arasında istatiksel olarak anlamlı ilişki bulundu. miRNA-758 ile kolesterol ve hdl düzeyi arasında istatiksel olarak anlamlı ilişki bulundu. Hasta grubunda ventrikül çapı ve son HD'de yapılan UF açısından bakıldığında mi33 ile istatiksel olarak anlamlı ilşkili bulundu. miRNA-758 Na, WBC, PLT, ventrikül çapı ve son HD'de yapılan UF arasında istatiksel olarak anlamlı ilişki bulundu, EPO tedavisi alanlarda miRNA-370 istatiksel olarak anlamlı bulundu. miRNA-370 miRNA-335 ile korele bulundu. Sonuç: Daha önce benzer çalışmaların olmaması ve araştırılma aşaması dünya genelinde devam etmesinden dolayı mikroRNA'ların gelecekte hipertansiyonun etyo-patogenezinde araştırılması ve tedavisi için çalışmamız yol gösterici olabilir.
Objective: HT is the main risk factor for cardiovascular diseases affecting more than 1 billion of the human population in the world. MiRNA-mediated research is the most common among the new research types in the treatment of chronic diseases recently. Although their functions are well known in many diseases (cancer, metabolic syndromes and diabetes), they have very few roles associated with cardiovascular diseases and HT. Current studies suggest that miRNAs may have great functions in HT's etiology and mixed pathogenesis. Numerous studies have shown that microRNAs are involved in a variety of cellular processes, including cellular proliferation, apoptosis, differentiation, and the development of diseases. miRNAs can regulate gene expression at the post-transcription level, are expressed in many tumor types and the cardiovascular system, and play an important role in the formation and development of cardiovascular diseases. This research planned to review the function of miRNAs, their varying levels, and the role of mi'RNAs in the pathogenesis of hypertension in 50 hypertensive hemodialysis patients. Material and method: The study was approved by the Selcuk University Faculty of Medicine Ethics Committee's decision dated 16.10.2019 and numbered 2019/269. This study was carried out prospectively between 01.05.2020-07.05.2020 in Selcuk University Faculty of Medicine, Department of Internal Medicine, Department of Nephrology, Medical Genetics Department and Hemodialysis unit. All data were evaluated using SPSS 21.0 statistical software. In calculating the data, criteria such as number, percentage, average and standard deviation were used. Pearson correlation analysis was used to evaluate the relationship between numerical variables. Significance level p <0.005 was taken. Results: When all groups were analyzed, miRNA-370 was found to be significant in the control group and miRNA-758 was significant in the patient group. A statistically significant relationship was found between miRNA-33 and miRNA-370 and miRNA-335 and miRNA-758. A statistically significant relationship was found between miRNA-758 and cistolic blood pressure and diastolic blood pressure , and between miRNA-33 and phosphorus. A statistically significant relationship was found between miRNA-335 and phosphorus and PTH, and between miRNA-758 and Na and PLT. A statistically significant relationship was found between miRNA-758 and cholesterol and hdl levels. When viewed in terms of ventricular diameter and uf done in the last hd, it was found statistically significant correlation with mi33. A statistically significant relationship was found between miRNA-758 Na, WBC, PLT, ventricular diameter, and UF performed in the last HD, and miRNA-370 was statistically significant in those receiving EPO therapy. correlated with miRNA-370 miRNA-335. Conclusion: microRNAs may be a guide for the etio-pathogenesis investigation and treatment of hypertension in the future due to the lack of similar studies and the research phase continues worldwide.
Objective: HT is the main risk factor for cardiovascular diseases affecting more than 1 billion of the human population in the world. MiRNA-mediated research is the most common among the new research types in the treatment of chronic diseases recently. Although their functions are well known in many diseases (cancer, metabolic syndromes and diabetes), they have very few roles associated with cardiovascular diseases and HT. Current studies suggest that miRNAs may have great functions in HT's etiology and mixed pathogenesis. Numerous studies have shown that microRNAs are involved in a variety of cellular processes, including cellular proliferation, apoptosis, differentiation, and the development of diseases. miRNAs can regulate gene expression at the post-transcription level, are expressed in many tumor types and the cardiovascular system, and play an important role in the formation and development of cardiovascular diseases. This research planned to review the function of miRNAs, their varying levels, and the role of mi'RNAs in the pathogenesis of hypertension in 50 hypertensive hemodialysis patients. Material and method: The study was approved by the Selcuk University Faculty of Medicine Ethics Committee's decision dated 16.10.2019 and numbered 2019/269. This study was carried out prospectively between 01.05.2020-07.05.2020 in Selcuk University Faculty of Medicine, Department of Internal Medicine, Department of Nephrology, Medical Genetics Department and Hemodialysis unit. All data were evaluated using SPSS 21.0 statistical software. In calculating the data, criteria such as number, percentage, average and standard deviation were used. Pearson correlation analysis was used to evaluate the relationship between numerical variables. Significance level p <0.005 was taken. Results: When all groups were analyzed, miRNA-370 was found to be significant in the control group and miRNA-758 was significant in the patient group. A statistically significant relationship was found between miRNA-33 and miRNA-370 and miRNA-335 and miRNA-758. A statistically significant relationship was found between miRNA-758 and cistolic blood pressure and diastolic blood pressure , and between miRNA-33 and phosphorus. A statistically significant relationship was found between miRNA-335 and phosphorus and PTH, and between miRNA-758 and Na and PLT. A statistically significant relationship was found between miRNA-758 and cholesterol and hdl levels. When viewed in terms of ventricular diameter and uf done in the last hd, it was found statistically significant correlation with mi33. A statistically significant relationship was found between miRNA-758 Na, WBC, PLT, ventricular diameter, and UF performed in the last HD, and miRNA-370 was statistically significant in those receiving EPO therapy. correlated with miRNA-370 miRNA-335. Conclusion: microRNAs may be a guide for the etio-pathogenesis investigation and treatment of hypertension in the future due to the lack of similar studies and the research phase continues worldwide.
Açıklama
Anahtar Kelimeler
miRNA, HT, miRNA-33, miRNA-370, miRNA-335, miRNA-758
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Ahmadlı, N. (2020). Hipertansif hemodiyaliz hastalarında mi'RNA'ların fonksiyonu ve mi'RNA'ların hipertansiyon patogenezindeki rolü. (Uzmanlık Tezi). Selçuk Üniversitesi, Tıp Fakültesi, Konya.
