Nigella Sativa (Çörek Otu) Tohum Yağının Bisphenol A Uygulanan Ratlarda Bazı Sitokinler ve Antioksidan Sistem Üzerine Etkisi
Yükleniyor...
Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada insan ve çevre sağlığı üzerinde zararlı etkileri olan BPA’ya karşı antioksidan ve
anti-inflamatuar etkileri olduğu bildirilen Nigella Sativa (Çörek Otu) tohum yağının sitokin ve
antioksidan parametreleri (Tnf-α, IL-6, IL-10, MDA, GSH ve SOD) üzerine etkisinin belirlenmesi
amaçlanmıştır.
Araştırmada, sağlıklı haldeki 30 tane 6-8 haftalık erkek Wistar Albino rat kullanıldı. Denemede
kullanılan ratlar; kontrol (K), BPA, Nigella Sativa (NS) ve BPA+ Nigella Sativa (BPAN) olmak üzere
4 eşit gruba ayrıldı. Denemeye başlamadan önce hayvanların canlı olarak ağırlıkları belirlendi. 4 haftalık
deneme süresi sırasında BPA ve BPAN gruplarına 100 mg/kg canlı ağırlığında BPA, oral olarak
uygulandı. NS ve BPAN grubundaki ratlara ise 30 gün süreyle 5 ml/kg. N. Sativa tohum yağı oral olarak
verildi.
Deneme sonunda genel anestezi altında (thiopental anestezisi, 40 mg/kg) ve kardiak punksiyon
ile kalpten alınan kan örneklerinden IL-6, IL-10, Tnf-α, MDA, SOD ve GSH düzeyleri belirlendi.
Çalışma süresince deneklerden elde edilen canlı ağırlık verileri incelendiğinde BPA ve BPAN
grubundaki verilerin K ve N gruplarına kıyasla önemli düzeyde düşük olduğu gözlendi (p<0.05). BPA
grubunda bulunan ratlardaki MDA düzeyinde diğer üç gruba göre belirgin bir artış belirlendi (p<0.05).
BPA toksikasyonunu önlemek veya oluşturduğu negatif etkileri azaltmak amacıyla eş zamanlı olarak
N. Sativa uygulanan BPAN grubundaki MDA düzeyinin ise BPA grubuna göre azalma eğiliminde
olduğu belirlendi. GSH düzeyinin istatistiksel olarak en düşük düzeyinin BPA grubunda olduğu
gözlemlendi (p<0.05). BPAN grubundaki antioksidanlara (GSH ve SOD) ait düzeylerin ise toksikasyon
oluşturulan deneme grubuna (BPA) göre arttığı belirlendi. TNF-α ve IL-6 düzeylerinin BPA grubunda
en yüksek değerde olduğu tespit edildi (p<0.05). Ancak aynı parametrelere ait veriler, N. Sativa
uygulaması yapılan BPAN grubunda anlamlı olarak azaldı (p<0.05). Bununla birlikte IL-10 düzeyi BPA
grubunda belirgin olarak azalırken, BPAN grubunda ise BPA grubuna göre arttığı tespit edildi (p<0.05).
Sonuç olarak BPA'nın yol açtığı oksidatif stres ve toksisiteye karşı korumada N. Sativa yağının
güçlü antioksidatif aktivite ve anti-inflamatuar etkisinin bakılan parametreler açısından pozitif etkiler
gösterdiği, elde edilen veriler sonucunda BPA’nın zararlı etkilerini önleme ya da azaltmada etkili
olduğu düşünülmektedir.
In this study, it was aimed to determine the effect of Nigella Sativa (Black Cumin), which is reported to have antioxidant and anti-inflammatory effects against BPA, which has harmful effects on human and environmental health, cytokine and antioxidant parameters (Tnf-α, IL-6, IL-10, MDA, GSH and SOD). In the study, 30 healthy 6-8 weeks old male Wistar Albino rats were used. The rats used in the experiment were divided into 4 equal groups as control (K), BPA, Nigella Sativa (NS) and BPA+ Nigella Sativa (BPAN). Before starting the experiment, the live weights of the animals were determined. During the 4-week trial, BPA and BPAN groups were given orally 100 mg/kg body weight BPA throughout the trial. 5 ml/kg N. Sativa seed oil was given orally to the rats in the NS and BPAN groups for 30 days. At the end of the experiment, IL-6, IL-10, Tnf-α, MDA, SOD and GSH levels were determined from blood samples taken from the heart under general anesthesia (thiopental anesthesia, 40 mg/kg) and cardiac puncture. When the body weight data obtained from the subjects during the study were examined, it was observed that the data in the BPA and BPAN groups were significantly lower than the K and N groups (p<0.05). There was a significant increase in MDA level in rats in the BPA group compared to the other three groups (p<0.05). It was determined that the MDA level tended to decrease in the BPAN group, in which N. Sativa was administered concurrently to prevent BPA toxication or to reduce its negative effects, compared to the BPA group. It was observed that the GSH level was statistically the lowest in the BPA group (p<0.05). It was determined that the levels of antioxidants (GSH and SOD) in the BPAN group were increased compared to the toxicated experimental group (BPA). TNF-α and IL-6 levels were found to be highest in the BPA group (p<0.05). However, the data for the same parameters were significantly decreased in the BPAN group treated with N. Sativa (p<0.05). On the other hand, IL-10 level was significantly decreased in the BPA group, but increased in the BPAN group compared to the BPA group (p<0.05). As a result, the strong antioxidative activity and anti-inflammatory effect of N. Sativa oil is thought to have positive effects in protection against oxidative stress and toxicity caused by BPA in terms of the parameters examined, as a result of the data obtained, the strong antioxidative activity and anti-inflammatory effect of N. Sativa oil is effective in preventing or reducing the harmful effects of BPA.
In this study, it was aimed to determine the effect of Nigella Sativa (Black Cumin), which is reported to have antioxidant and anti-inflammatory effects against BPA, which has harmful effects on human and environmental health, cytokine and antioxidant parameters (Tnf-α, IL-6, IL-10, MDA, GSH and SOD). In the study, 30 healthy 6-8 weeks old male Wistar Albino rats were used. The rats used in the experiment were divided into 4 equal groups as control (K), BPA, Nigella Sativa (NS) and BPA+ Nigella Sativa (BPAN). Before starting the experiment, the live weights of the animals were determined. During the 4-week trial, BPA and BPAN groups were given orally 100 mg/kg body weight BPA throughout the trial. 5 ml/kg N. Sativa seed oil was given orally to the rats in the NS and BPAN groups for 30 days. At the end of the experiment, IL-6, IL-10, Tnf-α, MDA, SOD and GSH levels were determined from blood samples taken from the heart under general anesthesia (thiopental anesthesia, 40 mg/kg) and cardiac puncture. When the body weight data obtained from the subjects during the study were examined, it was observed that the data in the BPA and BPAN groups were significantly lower than the K and N groups (p<0.05). There was a significant increase in MDA level in rats in the BPA group compared to the other three groups (p<0.05). It was determined that the MDA level tended to decrease in the BPAN group, in which N. Sativa was administered concurrently to prevent BPA toxication or to reduce its negative effects, compared to the BPA group. It was observed that the GSH level was statistically the lowest in the BPA group (p<0.05). It was determined that the levels of antioxidants (GSH and SOD) in the BPAN group were increased compared to the toxicated experimental group (BPA). TNF-α and IL-6 levels were found to be highest in the BPA group (p<0.05). However, the data for the same parameters were significantly decreased in the BPAN group treated with N. Sativa (p<0.05). On the other hand, IL-10 level was significantly decreased in the BPA group, but increased in the BPAN group compared to the BPA group (p<0.05). As a result, the strong antioxidative activity and anti-inflammatory effect of N. Sativa oil is thought to have positive effects in protection against oxidative stress and toxicity caused by BPA in terms of the parameters examined, as a result of the data obtained, the strong antioxidative activity and anti-inflammatory effect of N. Sativa oil is effective in preventing or reducing the harmful effects of BPA.
Açıklama
Anahtar Kelimeler
Bisfenol A, N.Sativa, oksidatif stres, sitokin, bisphenol A, cytokines, oxidative stress
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Açıkbaş, M., (2022). Nigella Sativa (Çörek Otu) Tohum Yağının Bisphenol A Uygulanan Ratlarda Bazı Sitokinler ve Antioksidan Sistem Üzerine Etkisi. (Yüksek Lisans Tezi). Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, Konya.
