Endemik Beyşehir Kababurun balığı,Chondrostoma beysehirense (Bogutskaya, 1997) üzerine sitogenetik araştırmalar
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Dosyalar
Tarih
2016-06-01
Yazarlar
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Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, Beyşehir Gölü'ndeki Kababurun Balığı, Chondrostoma beysehirense'nin 10 örneğine standart giemsa boyama, gümüş NOR (Ag-NOR) boyama ve C-bantlama uygulanarak, onların ayrıntılı sitogenetik özellikleri araştırılmıştır. Diploid kromozom sayısının 50 ve kromozom karyotip analizinde kromozomların ise metasentrik, submetasentrik/subtelosentrik ve akrosentrik olduğu tespit edilmiştir. Morfolojik olarak farklılaşmış eşey kromozomları tespit edilememiştir. Altıncı submetasentrik kromozomun kısa kolunda ikincil bir boğum gözlenmiştir. Bazı kromozomlarda sentromerik ve perisentromerik C-bantlara sahiptir ve altıncı submetasentrik kromozom çiftinin kısa kolunun tamamının C-pozitif olduğu belirlenmiştir. Altıncı submetasentrik kromozom çiftinin kısa kolunda aktif NOR bölgeleri gözlemlendi ve bu aktif NOR'lar ile C-heterokromatinin ilişkili olduğu bulunmuştur.Bu NOR incelenen tüm örneklerde kaydedilmiştir ve homolog kromozomların her ikisi de homomorfiktir. Gelecekte Chondrostoma türlerinin sistematiği moleküler ve FISH tekniği ile daha iyi anlaşılacaktır.
In this study, conventional giemsa staining, Ag-NOR and C-banding were applied to ten specimens of Beysehir nase, Chondrostoma beysehirense in Lake Beysehir and their detailed cytogenetic properties were investigated. The diploid number of chromosomes was 50 and the chromosomes in karyotypes were distinguished as metacentric, submetacentric/subtelocentric and acrocentric. Morphologically differentiated sex chromosomes could not be recognized. A secondary constriction was observed on the short arm of the sixth submetacentric chromosome pair. Some chromosomes had centromeric and pericentromeric C-bands and the short arm of the sixth submetacentric chromosome pair was entirely C-positive. The active NOR was localized on the short arm of the sixth submetacentric chromosome pair and this active NOR was associated with a large C-heterochromatin region. This NOR was recorded in all specimens examined and it occurred always in the homomorphic state on both homologous chromosomes. Systematics of Chondrostoma species will be better understood by using both molecular and FISH techniques in the future.
In this study, conventional giemsa staining, Ag-NOR and C-banding were applied to ten specimens of Beysehir nase, Chondrostoma beysehirense in Lake Beysehir and their detailed cytogenetic properties were investigated. The diploid number of chromosomes was 50 and the chromosomes in karyotypes were distinguished as metacentric, submetacentric/subtelocentric and acrocentric. Morphologically differentiated sex chromosomes could not be recognized. A secondary constriction was observed on the short arm of the sixth submetacentric chromosome pair. Some chromosomes had centromeric and pericentromeric C-bands and the short arm of the sixth submetacentric chromosome pair was entirely C-positive. The active NOR was localized on the short arm of the sixth submetacentric chromosome pair and this active NOR was associated with a large C-heterochromatin region. This NOR was recorded in all specimens examined and it occurred always in the homomorphic state on both homologous chromosomes. Systematics of Chondrostoma species will be better understood by using both molecular and FISH techniques in the future.
Açıklama
Anahtar Kelimeler
C-bantlama, Ag-NOR bantlama, Kromozom, Karyotip, C-banding, Ag-NOR banding, Karyotype, Chromosome
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Gündoğdu, H. (2016). Endemik Beyşehir Kababurun balığı,Chondrostoma beysehirense (Bogutskaya, 1997) üzerine sitogenetik araştırmalar. Selçuk Üniversitesi, Yayımlanmış yüksek lisans tezi, Konya.
